1CQI
Crystal Structure of the Complex of ADP and MG2+ with Dephosphorylated E. Coli Succinyl-CoA Synthetase
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 283 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-06-08 |
| Detector | FUJI |
| Spacegroup name | P 43 2 2 |
| 格子定数 [Å] | 99.860, 99.860, 407.150 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 20.000 - 3.300 |
| R因子 | 0.191 |
| Rwork | 0.191 |
| R-free | 0.24700 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.017 |
| 結合角の平均二乗偏差(RMSD) [度] | 25.000 * |
| Data reduction software | WEIS |
| Data scaling software | SCALKB2 |
| Refinement software | CNS |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 20.000 | 3.500 |
| 分解能 [Å] (高) | 3.300 | 3.300 |
| Rmerge_l_obs | 0.164 | 0.378 |
| Total number of observations | 195264 * | |
| 独立反射数 | 31753 | 15389 * |
| 完全性 [%] | 99.0 | 98.4 |
| 冗長性 | 6.3 | 3.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Microdialysis * | 7.3 | 21 * | AMMONIUM SULFATE, POTASSIUM PHOSPHATE, COENZYME A, pH 7.30, VAPOR DIFFUSION, HANGING DROP |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | 1 | ammonium sulfate | 1.9 (M) | |
| 2 | 1 | 1 | potassium phosphate | 0.1 (M) | |
| 3 | 1 | 1 | dithiothreitol | 1 (mM) | |
| 4 | 1 | 1 | CoA | 0.1 (mM) | |
| 5 | 1 | 1 | EDTA | 0.1 (mM) |
