1AXS
MATURE OXY-COPE CATALYTIC ANTIBODY WITH HAPTEN
Experimental procedure
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 94 |
Detector technology | IMAGE PLATE |
Collection date | 1997-01 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.780, 81.500, 128.200 |
Unit cell angles | 90.00, 93.43, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.600 |
R-factor | 0.2 |
Rwork | 0.200 |
R-free | 0.30500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6fab |
RMSD bond length | 0.008 |
RMSD bond angle | 30.100 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.851) |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.060 | 0.142 |
Number of reflections | 24768 | |
<I/σ(I)> | 16 | 6 |
Completeness [%] | 90.9 | 87.5 |
Redundancy | 4 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.6 | PROTEIN WAS CRYSTALLIZED FROM 25%PEG 1000, 0.1 M SODIUM ACETATE, PH 4.6, 0.3 M CDCL2 AND 0.1 M AMMONIUM SULFATE IN THE PRESENCE OF 2MM HAPTEN. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | PEG1000 | 25 (%) | |
2 | 1 | drop | sodium acetate | 0.1 (M) | |
3 | 1 | drop | 0.3 (M) | ||
4 | 1 | drop | ammonium sulfate | 0.1 (M) | |
5 | 1 | drop | hapten 1 | 2 (mM) |