Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-12 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| 格子定数 [Å] | 41.980, 41.980, 88.920 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 25.000 - 1.700 |
| R因子 | 0.215 |
| Rwork | 0.215 |
| R-free | 0.25300 |
| Structure solution method | MULTI-WAVELENGTH ANOMALOUS DISPERSION |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.005 |
| 結合角の平均二乗偏差(RMSD) [度] | 20.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.843) |
| Refinement software | X-PLOR (3.843) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 25.000 | 1.730 |
| 分解能 [Å] (高) | 1.700 | 1.700 |
| Rmerge_l_obs | 0.048 * | |
| 独立反射数 | 9307 | |
| <I/σ(I)> | 15 | 5 |
| 完全性 [%] | 99.6 | 98.6 |
| 冗長性 | 4.7 * |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 4 * | CRYSTALS OF CA(151-231) WERE GROWN AT 4C IN 4 MICROLITER SITTING DROPS CONTAINING A 1:1 MIXTURE OF PROTEIN SOLUTION (2.1 MM CA(151-231) IN 10MM TRIS (PH 8.0) AND 2 MM 2-MERCAPTOETHANOL) AND RESERVOIR SOLUTION (2.0 M AMMONIUM SULFATE), vapor diffusion - sitting drop, temperature 277K |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein | 5.1 (mM) | |
| 2 | 1 | drop | Tris | 10 (mM) | |
| 3 | 1 | drop | 2-mercaptoethanol | 2 (mM) | |
| 4 | 1 | reservoir | PEG8000 | 20 (%) | |
| 5 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
| 6 | 1 | reservoir | calcium acetate | 0.2 (M) |
