1A79

CRYSTAL STRUCTURE OF THE TRNA SPLICING ENDONUCLEASE FROM METHANOCOCCUS JANNASCHII

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-09-07
Detector	FUJI
Wavelength(s)	1.02, 1.54
Spacegroup name	P 21 21 21
Unit cell lengths	61.950, 80.040, 193.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.280
R-factor	0.2
Rwork	0.200
R-free	0.26700
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	27.400 *
Data reduction software	HKL
Data scaling software	HKL
Phasing software	CCP4
Refinement software	X-PLOR (3.857)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.360
High resolution limit [Å]	2.300	2.280
Rmerge	0.054	0.167
Total number of observations	165549 *
Number of reflections	75778
<I/σ(I)>	10.3	2.4
Completeness [%]	90.4	65.4
Redundancy	2.2	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6	22 *	pH 6.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		1 (M)
2	1	reservoir	ammonium sulfate	20-80 (mM)