1A43

STRUCTURE OF THE HIV-1 CAPSID PROTEIN DIMERIZATION DOMAIN AT 2.6A RESOLUTION

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	300
Detector technology	IMAGE PLATE
Collection date	1997-09
Detector	MARRESEARCH
Spacegroup name	I 41
格子定数 [Å]	60.410, 60.410, 60.430
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	20.000 - 2.600
R因子	0.223
Rwork	0.223
R-free	0.28100
結合長の平均二乗偏差(RMSD) [Å]	0.009
結合角の平均二乗偏差(RMSD) [度]	21.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.843)
Refinement software	X-PLOR (3.843)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	20.000
分解能 [Å] (高)	2.600
Rmerge_l_obs	0.072 *	0.529 *
Total number of observations	21325 *
独立反射数	3166 *
完全性 [%]	91.3 *	92.5 *

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	Vapor diffusion, sitting drop *	5		drop contained 1:1 mixture of protein and reservoir solution *

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	drop	protein	2.1 (mM)
2	1	drop	Tris-HCl	10 (mM)
3	1	drop	2-mercaptoethanol	2 (mM)
4	1	reservoir	ammonium sulfate	0.7 (M)
5	1	reservoir	lithium sulfate	1.0 (M)
6	1	reservoir	HEPES	0.1 (M)