1A2D

PYRIDOXAMINE MODIFIED MURINE ADIPOCYTE LIPID BINDING PROTEIN

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1996-06
Detector	SIEMENS
Spacegroup name	P 21 21 21
格子定数 [Å]	80.080, 96.250, 49.800
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	20.000 * - 2.600 *
R因子	0.196
Rwork	0.196
R-free	0.28500 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lib
結合長の平均二乗偏差(RMSD) [Å]	0.007 *
結合角の平均二乗偏差(RMSD) [度]	1.395 *
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	X-PLOR
Refinement software	X-PLOR (3.843)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	20.000 *	2.520
分解能 [Å] (高)	2.600 *	2.600 *
Rmerge_l_obs	0.095 *	0.302 *
Total number of observations	76938 *
独立反射数	8916 *
<I/σ(I)>	13.1	2.3
完全性 [%]	94.0 *	78.05 *
冗長性	4.5	2

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	Vapor diffusion, hanging drop *	7.5 *		pH 6.5

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	drop	protein	10 (mg/ml)
2	1	drop	HEPES	20 (mM)
3	1	reservoir	PEG400	20-30 (%)
4	1	reservoir		0.1-0.25 (M)
5	1	reservoir	Tris-HCl	0.1 (M)