1A2A
AGKISTROTOXIN, A PHOSPHOLIPASE A2-TYPE PRESYNAPTIC NEUROTOXIN FROM AGKISTRODON HALYS PALLAS
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 291 |
Detector technology | AREA DETECTOR |
Collection date | 1994-09 |
Detector | SIEMENS |
Spacegroup name | P 1 21 1 |
格子定数 [Å] | 108.950, 85.227, 71.054 |
格子定数 [度] | 90.00, 109.66, 90.00 |
精密化法
残基 | 6.000 - 2.800 |
R因子 | 0.207 |
Rwork | 0.207 |
R-free | 0.28500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1psj |
結合長の平均二乗偏差(RMSD) [Å] | 0.008 |
結合角の平均二乗偏差(RMSD) [度] | 26.900 * |
Data reduction software | XENGEN |
Data scaling software | XENGEN |
Phasing software | X-PLOR (3.851) |
Refinement software | X-PLOR (3.851) |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 100.000 | 2.740 |
分解能 [Å] (高) | 2.590 * | 2.580 |
Rmerge_l_obs | 0.064 | 0.410 |
独立反射数 | 32147 | |
<I/σ(I)> | 9.42 | 1.3 |
完全性 [%] | 84.4 * | 26 |
冗長性 | 1.9 | 1.5 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | Vapor diffusion, hanging drop * | 4.5 | 18 * | pH 4.5 |
文献の結晶化試薬
ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | MPD | 60 (%) | |
3 | 1 | drop | ammonium sulfate | 0.6 (M) | |
4 | 1 | drop | octyl-beta-D-glucopyranoside | 1 (%) | |
5 | 1 | drop | 0.6 (M) | ||
6 | 1 | drop | 0.1 (M) | ||
7 | 1 | reservoir | MPD | 60 (%) |