1A1V
HEPATITIS C VIRUS NS3 HELICASE DOMAIN COMPLEXED WITH SINGLE STRANDED SDNA
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-05-15 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 21 21 2 |
| 格子定数 [Å] | 73.100, 117.500, 63.400 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 6.000 - 2.200 |
| R因子 | 0.228 |
| Rwork | 0.228 |
| R-free | 0.28700 |
| Structure solution method | MIR |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
| 結合角の平均二乗偏差(RMSD) [度] | 24.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.843) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 35.000 | 2.180 |
| 分解能 [Å] (高) | 2.100 | 2.100 |
| Rmerge_l_obs | 0.041 | 0.355 |
| 独立反射数 | 31255 | |
| <I/σ(I)> | 29.7 | 5.9 |
| 完全性 [%] | 95.5 | 81.7 |
| 冗長性 | 3.2 | 2.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion, hanging drop * | 8 | pH 8.0 |
結晶化試薬
| ID | 結晶ID | 濃度 | 試薬名 | 濃度 (単位) | 詳細 |
| 1 | 1 | 1 | PEG 6000 | ||
| 2 | 1 | 1 | LI2SO4 | ||
| 3 | 1 | 1 | 2-MERCAPTOETHANOL | ||
| 4 | 1 | 1 | TRIS | ||
| 5 | 1 | 2 | PEG 6000 |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
| 2 | 1 | reservoir | 0.2 (M) | ||
| 3 | 1 | reservoir | PEG6000 | 18 (%) | |
| 4 | 1 | reservoir | beta-mercaptoethanol | 8 (mM) |
