1A0G
L201A MUTANT OF D-AMINO ACID AMINOTRANSFERASE COMPLEXED WITH PYRIDOXAMINE-5'-PHOSPHATE
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1996-05-29 |
Detector | RIGAKU |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.340, 91.760, 88.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1daa |
RMSD bond length | 0.009 |
RMSD bond angle | 23.800 * |
Data reduction software | PROCESS |
Data scaling software | PROCESS |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 88.690 | 1.900 |
High resolution limit [Å] | 1.790 | 1.790 |
Rmerge | 0.090 | 0.356 |
Number of reflections | 48469 | |
<I/σ(I)> | 7.5 | 1.58 |
Completeness [%] | 80.8 | 50.6 |
Redundancy | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 9 | drop solution was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | D-alanine | 50 (mM) | |
2 | 1 | drop | alpha-ketoglutartrate | 50 (mM) | |
3 | 1 | reservoir | Tris-HCl | 100 (mM) | |
4 | 1 | reservoir | PEG3350 | 29-33 (%(w/v)) | |
5 | 1 | reservoir | sodium acetate | 300-700 (mM) | |
6 | 1 | reservoir | sodium azide | 5 (mM) |