1A0G
L201A MUTANT OF D-AMINO ACID AMINOTRANSFERASE COMPLEXED WITH PYRIDOXAMINE-5'-PHOSPHATE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-05-29 |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 77.340, 91.760, 88.690 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 50.000 - 2.000 |
| R因子 | 0.216 |
| Rwork | 0.216 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1daa |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.009 |
| 結合角の平均二乗偏差(RMSD) [度] | 23.800 * |
| Data reduction software | PROCESS |
| Data scaling software | PROCESS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 88.690 | 1.900 |
| 分解能 [Å] (高) | 1.790 | 1.790 |
| Rmerge_l_obs | 0.090 | 0.356 |
| 独立反射数 | 48469 | |
| <I/σ(I)> | 7.5 | 1.58 |
| 完全性 [%] | 80.8 | 50.6 |
| 冗長性 | 4.7 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion, hanging drop * | 9 | drop solution was mixed with an equal volume of reservoir solution * |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | D-alanine | 50 (mM) | |
| 2 | 1 | drop | alpha-ketoglutartrate | 50 (mM) | |
| 3 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 4 | 1 | reservoir | PEG3350 | 29-33 (%(w/v)) | |
| 5 | 1 | reservoir | sodium acetate | 300-700 (mM) | |
| 6 | 1 | reservoir | sodium azide | 5 (mM) |
