1A0E
XYLOSE ISOMERASE FROM THERMOTOGA NEAPOLITANA
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-21 |
| Temperature [K] | 293 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1994-06 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | C 2 2 21 |
| 格子定数 [Å] | 161.790, 121.870, 98.860 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 12.500 - 2.700 |
| R因子 | 0.18 |
| Rwork | 0.180 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | XYLOSE ISOMERASE FROM THERMOANAEROBACTERIUM THERMOSULFURIGENES |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.009 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.500 |
| Data reduction software | MADNES |
| Data scaling software | CCP4 |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 12.500 | 2.880 |
| 分解能 [Å] (高) | 2.700 | 2.700 |
| Rmerge_l_obs | 0.076 | 0.158 |
| 独立反射数 | 26137 | |
| <I/σ(I)> | 7.1 | 4.3 |
| 完全性 [%] | 96.3 | 93.9 |
| 冗長性 | 2.3 | 1.9 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | 7 | PROTEIN WAS CRYSTALLIZED FROM 14% JEFFAMINE ED 4000, 5 MM MGSO4, 0.5 MM COCL2, 50 MM MOPS, PH 7.0 (FOR DETAILS SEE REFERENCE 1). |
