PDB-5y9f: Crystal structure of HPV59 pentamer in complex with the Fab fragm...

Basic information

• Entry: Database: PDB / ID: 5y9f
• Title: Crystal structure of HPV59 pentamer in complex with the Fab fragment of antibody 28F10

Components

• Major capsid protein L1
• heavy chain of Fab fragment of antibody 28F10
• light chains of Fab fragment of antibody 28F10

• Keywords: VIRAL PROTEIN/IMMUNE SYSTEM / capsid protein / immune complex / STRUCTURAL PROTEIN / VIRAL PROTEIN-IMMUNE SYSTEM complex

Function / homology

Function:

T=7 icosahedral viral capsid / endocytosis involved in viral entry into host cell / host cell nucleus / virion attachment to host cell / structural molecule activity

Domain/homology:

Major capsid L1 (late) superfamily, Papillomavirus / Major capsid L1 (late) protein, Papillomavirus / Major capsid L1 (late) superfamily, Papillomavirus / L1 (late) protein / Polyomavirus Vp1; Chain A / Double-stranded DNA virus, group I, capsid / Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta

Component:

Major capsid protein L1

• Biological species: Human papillomavirus type 59; Mus musculus (house mouse)
• Method: X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 3.35 Å
• Authors: Li, S.W. / Li, Z.H.

Funding support

China, 3items

Organization	Grant number	Country
National Natural Science Foundation of China	31670935	China
National Natural Science Foundation of China	81671645	China
National Natural Science Foundation of China	81571996	China

• Citation: Journal: mBio / Year: 2017; Title: Crystal Structures of Two Immune Complexes Identify Determinants for Viral Infectivity and Type-Specific Neutralization of Human Papillomavirus.; Authors: Zhihai Li / Daning Wang / Ying Gu / Shuo Song / Maozhou He / Jingjie Shi / Xinlin Liu / Shuangping Wei / Jinjin Li / Hai Yu / Qingbing Zheng / Xiaodong Yan / Timothy S Baker / Jun Zhang / Jason S McLellan / Shaowei Li / Ningshao Xia / ; Abstract: Persistent, high-risk human papillomavirus (HPV) infection is the primary cause of cervical cancer. Neutralizing antibodies elicited by L1-only virus-like particles (VLPs) can block HPV infection; however, the lack of high-resolution structures has limited our understanding of the mode of virus infection and the requirement for type specificity at the molecular level. Here, we describe two antibodies, A12A3 and 28F10, that specifically bind to and neutralize HPV58 and HPV59, respectively, through two distinct binding stoichiometries. We show that the epitopes of A12A3 are clustered in the DE loops of two adjacent HPV58 L1 monomers, whereas 28F10 recognizes the HPV59 FG loop of a single monomer. Via structure-based mutagenesis and analysis of antibody binding, we further identified the residues HPV58 D154, S168, and N170 and HPV59 M267, Q270, E273, Y276, K278, and R283, which play critical roles in virus infection. By substituting these strategic epitope residues into other HPV genotypes, we could then redirect the type-specific binding of the antibodies to these genotypes, thus highlighting the importance of these specific residues, HPV58 R161, S168, and N308 and HPV59 Q270, E273, and D281. Overall, our findings provide molecular insights into potential structural determinants of HPV required for infectivity and type specificity. High-risk human papillomaviruses (HPVs) are considered the major causative pathogens of cancers that affect epithelial mucosa, such as cervical cancer. However, because of the lack of high-resolution structural information on the sites of neutralization, we have yet to determine the precise mode of HPV infection and how different types of HPV cause infection. Our crystal structures in this study have uncovered discrete binding stoichiometries for two different antibodies. We show that one A12A3 Fab binds to the center of one HPV58 pentamer, whereas five 28F10 Fabs bind along the top fringe of one HPV59 pentamer. Furthermore, through targeted epitope analysis, we show that 6 to 7 discontinuous residues of the L1 major capsid protein of HPV are determinants, at least in part, for virus infection and type specificity. This knowledge will help us to unravel the process of HPV infection and can potentially be used to drive the development of therapeutics that target neutralization-sensitive sites.

History

Deposition	Aug 24, 2017	Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0	Oct 25, 2017	Provider: repository / Type: Initial release

Assembly

Deposited unit

A: Major capsid protein L1

B: Major capsid protein L1

C: Major capsid protein L1

D: Major capsid protein L1

E: Major capsid protein L1

F: Major capsid protein L1

G: Major capsid protein L1

H: Major capsid protein L1

I: Major capsid protein L1

J: Major capsid protein L1

K: light chains of Fab fragment of antibody 28F10

L: heavy chain of Fab fragment of antibody 28F10

O: light chains of Fab fragment of antibody 28F10

P: heavy chain of Fab fragment of antibody 28F10

Q: light chains of Fab fragment of antibody 28F10

R: heavy chain of Fab fragment of antibody 28F10

S: light chains of Fab fragment of antibody 28F10

T: heavy chain of Fab fragment of antibody 28F10

M: light chains of Fab fragment of antibody 28F10

N: heavy chain of Fab fragment of antibody 28F10

c: light chains of Fab fragment of antibody 28F10

d: heavy chain of Fab fragment of antibody 28F10

V: heavy chain of Fab fragment of antibody 28F10

W: light chains of Fab fragment of antibody 28F10

X: heavy chain of Fab fragment of antibody 28F10

Y: light chains of Fab fragment of antibody 28F10

Z: heavy chain of Fab fragment of antibody 28F10

a: light chains of Fab fragment of antibody 28F10

b: heavy chain of Fab fragment of antibody 28F10

U: light chains of Fab fragment of antibody 28F10

Summary:

• 1.04 MDa, 30 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	1,040,009	30
Polymers	1,040,009	30
Non-polymers	0	0
Water	0

1

A: Major capsid protein L1

B: Major capsid protein L1

C: Major capsid protein L1

D: Major capsid protein L1

E: Major capsid protein L1

K: light chains of Fab fragment of antibody 28F10

L: heavy chain of Fab fragment of antibody 28F10

O: light chains of Fab fragment of antibody 28F10

P: heavy chain of Fab fragment of antibody 28F10

Q: light chains of Fab fragment of antibody 28F10

R: heavy chain of Fab fragment of antibody 28F10

S: light chains of Fab fragment of antibody 28F10

T: heavy chain of Fab fragment of antibody 28F10

M: light chains of Fab fragment of antibody 28F10

N: heavy chain of Fab fragment of antibody 28F10

Summary:

• defined by author
• 520 kDa, 15 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	520,005	15
Polymers	520,005	15
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

2

F: Major capsid protein L1

G: Major capsid protein L1

H: Major capsid protein L1

I: Major capsid protein L1

J: Major capsid protein L1

c: light chains of Fab fragment of antibody 28F10

d: heavy chain of Fab fragment of antibody 28F10

V: heavy chain of Fab fragment of antibody 28F10

W: light chains of Fab fragment of antibody 28F10

X: heavy chain of Fab fragment of antibody 28F10

Y: light chains of Fab fragment of antibody 28F10

Z: heavy chain of Fab fragment of antibody 28F10

a: light chains of Fab fragment of antibody 28F10

b: heavy chain of Fab fragment of antibody 28F10

U: light chains of Fab fragment of antibody 28F10

Summary:

• defined by author
• 520 kDa, 15 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	520,005	15
Polymers	520,005	15
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Unit cell

Length a, b, c (Å)	116.265, 417.233, 134.942
Angle α, β, γ (deg.)	90.00, 111.02, 90.00
Int Tables number	4
Space group name H-M	P1211

Components

#1: Protein

A B C D E F G H I J
Major capsid protein L1

Details:

Mass: 55962.355 Da / Num. of mol.: 10 / Fragment: UNP RESIDUES 10-508 / Mutation: C175S
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Human papillomavirus type 59 / Gene: ORF putative L1, L1 / Production host: Escherichia coli (E. coli) / References: UniProt: Q81971

#2: Antibody

K O Q S M c W Y a U
light chains of Fab fragment of antibody 28F10

Details:

Mass: 24133.754 Da / Num. of mol.: 10 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse)

#3: Antibody

L P R T N d V X Z b
heavy chain of Fab fragment of antibody 28F10

Details:

Mass: 23904.793 Da / Num. of mol.: 10 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse)

Experimental details

Experiment

• Experiment: Method: X-RAY DIFFRACTION / Number of used crystals: 1

Sample preparation

• Crystal: Density Matthews: 2.94 ų/Da / Density % sol: 58.21 %
• Crystal grow: Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5.5 ; Details: 2% tacsimate, 0.1 M sodium citrate, pH 5.5, 17% (w/v) PEG 3350

Data collection

• Diffraction: Mean temperature: 80 K
• Diffraction source: Source: SYNCHROTRON / Site: SSRF / Beamline: BL17U1 / Wavelength: 0.9792 Å
• Detector: Type: ADSC QUANTUM 315 / Detector: CCD / Date: Sep 16, 2014
• Radiation: Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
• Radiation wavelength: Wavelength: 0.9792 Å / Relative weight: 1
• Reflection: Resolution: 3.35→50 Å / Num. obs: 161106 / % possible obs: 94.5 % / Redundancy: 3.2 % / Net I/σ(I): 8.5

Processing

Software

Name	Version	Classification
PHENIX	(1.11.1_2575)	refinement
HKL-2000		data scaling
PHASER		phasing

Refinement

Resolution: 3.35→36.528 Å / SU ML: 0.45 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 27.03 / Stereochemistry target values: ML

	Rfactor	Num. reflection	% reflection
Rfree	0.2612	8042	4.99 %
Rwork	0.23	-	-
obs	0.2316	161016	94.05 %

• Solvent computation: Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Refinement step

Cycle: LAST / Resolution: 3.35→36.528 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	66832	0	0	0	66832

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.002	68573
X-RAY DIFFRACTION	f_angle_d	0.649	93356
X-RAY DIFFRACTION	f_dihedral_angle_d	13.11	40741
X-RAY DIFFRACTION	f_chiral_restr	0.045	10376
X-RAY DIFFRACTION	f_plane_restr	0.004	11946

LS refinement shell

Resolution (Å)	Rfactor Rfree	Num. reflection Rfree	Rfactor Rwork	Num. reflection Rwork	Refine-ID	% reflection obs (%)
3.3496-3.3876	0.3636	214	0.3262	4444	X-RAY DIFFRACTION	81
3.3876-3.4275	0.322	281	0.3037	5109	X-RAY DIFFRACTION	94
3.4275-3.4692	0.3403	259	0.2981	5087	X-RAY DIFFRACTION	94
3.4692-3.5131	0.329	257	0.2973	5142	X-RAY DIFFRACTION	94
3.5131-3.5593	0.3424	244	0.2888	5129	X-RAY DIFFRACTION	95
3.5593-3.608	0.3458	251	0.2919	5088	X-RAY DIFFRACTION	94
3.608-3.6595	0.3105	278	0.2846	5129	X-RAY DIFFRACTION	94
3.6595-3.7141	0.3017	221	0.281	5121	X-RAY DIFFRACTION	95
3.7141-3.7721	0.3209	242	0.2672	5189	X-RAY DIFFRACTION	94
3.7721-3.8339	0.3012	262	0.2681	5138	X-RAY DIFFRACTION	95
3.8339-3.8999	0.2925	272	0.2586	5089	X-RAY DIFFRACTION	94
3.8999-3.9707	0.2798	259	0.2586	5133	X-RAY DIFFRACTION	94
3.9707-4.047	0.2964	251	0.243	5121	X-RAY DIFFRACTION	95
4.047-4.1295	0.266	275	0.2396	5117	X-RAY DIFFRACTION	94
4.1295-4.2192	0.2633	276	0.234	5102	X-RAY DIFFRACTION	94
4.2192-4.3172	0.2417	252	0.2236	5133	X-RAY DIFFRACTION	95
4.3172-4.4249	0.2553	276	0.2218	5078	X-RAY DIFFRACTION	95
4.4249-4.5444	0.2572	274	0.2188	5126	X-RAY DIFFRACTION	95
4.5444-4.6778	0.2392	294	0.2055	5123	X-RAY DIFFRACTION	94
4.6778-4.8285	0.2056	281	0.1914	5147	X-RAY DIFFRACTION	95
4.8285-5.0007	0.2284	270	0.1953	5091	X-RAY DIFFRACTION	94
5.0007-5.2003	0.2321	281	0.1997	5124	X-RAY DIFFRACTION	95
5.2003-5.4363	0.227	270	0.2054	5131	X-RAY DIFFRACTION	94
5.4363-5.7219	0.2521	261	0.2138	5124	X-RAY DIFFRACTION	94
5.7219-6.0788	0.2448	319	0.2312	5091	X-RAY DIFFRACTION	95
6.0788-6.5457	0.2773	258	0.2326	5123	X-RAY DIFFRACTION	94
6.5457-7.1999	0.2713	269	0.2224	5126	X-RAY DIFFRACTION	94
7.1999-8.2313	0.2551	288	0.2148	5123	X-RAY DIFFRACTION	95
8.2313-10.3316	0.2117	299	0.178	5176	X-RAY DIFFRACTION	95
10.3316-36.5305	0.2329	308	0.2026	5120	X-RAY DIFFRACTION	94

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°2)	L12 (°2)	L13 (°2)	L22 (°2)	L23 (°2)	L33 (°2)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å2)	T12 (Å2)	T13 (Å2)	T22 (Å2)	T23 (Å2)	T33 (Å2)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	0.7002	0.1057	-0.4385	0.2598	0.1058	0.6865	-0.0842	-0.0149	-0.0069	0.101	-0.0998	-0.0442	-0.0214	0.0434	-0.0079	0.4233	-0.129	0.0113	0.4164	-0.1756	0.6036	135.9502	112.7487	12.5429
2	0.3296	0.0895	0.035	0.5165	-0.1938	0.5703	-0.09	0.024	0.1105	-0.0621	0.0964	-0.1903	-0.027	0.081	0	0.3595	-0.0802	-0.0252	0.5646	-0.2231	0.6471	134.8705	110.0448	14.5291
3	0.613	0.7286	-0.5377	1.1554	-0.3849	0.8936	-0.218	-0.4677	-0.2373	-0.2389	-0.2926	-0.2813	-0.2841	0.4596	-1.1393	0.272	-0.0351	-0.3079	0.5632	-0.3494	0.5495	133.929	101.4604	44.9308
4	0.4254	0.1293	-0.3061	0.1387	0.0008	0.2968	-0.1261	-0.2766	-0.2389	0.0484	0.0392	0.1649	-0.2858	0.3322	-0.0066	0.5368	0.0037	-0.1248	0.6863	-0.1925	0.5418	135.7539	99.7497	43.6036
5	0.3435	-0.1863	-0.0701	0.328	0.0493	0.2112	-0.1223	0.1268	-0.1465	0.2077	-0.2248	0.2381	0.242	-0.1366	-0.0113	0.4698	-0.0252	0.0622	0.5711	-0.203	0.5227	121.6659	76.6551	31.5756
6	0.3383	-0.2922	-0.1375	0.1968	-0.0094	0.3258	-0.0506	-0.0665	0.0581	0.1949	0.1031	-0.0201	0.0123	-0.0032	-0	0.4752	0.0009	-0.0419	0.5307	-0.1452	0.4185	120.7423	84.4188	36.6135
7	0.1321	0.3554	0.0232	1.1145	0.0671	0.3682	-0.0811	-0.1329	-0.0447	-0.1474	0.0625	-0.6951	-0.0962	0.4976	-0.8698	0.1795	-0.1488	-0.3701	0.6915	-0.5287	0.1207	136.859	100.7516	41.6918
8	0.2384	-0.3265	-0.183	0.6067	0.0533	0.349	0.0465	0.1219	0.1716	-0.0596	0.0963	0.0359	0.0225	-0.0828	0.0115	0.4447	0.058	0.003	0.4902	-0.0308	0.365	98.4743	122.5529	-3.2998
9	0.2593	-0.1585	0.4224	0.292	-0.222	0.6469	0.0556	-0.0306	-0.2303	0.165	-0.0685	-0.0127	0.1495	0.1927	0	0.4297	-0.0368	0.0227	0.5314	-0.0805	0.3921	120.682	102.7975	-6.6134
10	0.355	0.0401	0.0465	0.6241	0.1778	-0.0478	0.0589	0.0235	0.1492	-0.1465	0.0718	-0.1301	-0.0924	0.1457	0	0.5425	-0.0689	0.0812	0.571	-0.156	0.394	119.7574	106.8447	-2.8631
11	0.1417	-0.0543	0.0092	0.184	0.115	-0.0184	-0.0723	0.0833	0.0486	-0.2652	0.1086	0.2361	-0.0809	0.0324	0	0.6431	0.0324	0.08	0.4603	-0.0528	0.442	97.7722	122.4469	-2.5358
12	0.652	-0.0332	0.2872	0.441	0.4424	0.3057	-0.1853	-0.125	0.0084	0.0941	0.2905	0.0852	0.1126	-0.1563	0.0776	0.3477	0.0216	0.0775	0.5621	-0.0895	0.3596	75.7924	99.8812	19.9133
13	0.301	0.3022	0.2666	0.5198	0.1395	0.3408	-0.0337	0.1777	-0.0355	-0.1685	-0.0216	-0.1246	-0.0058	0.2172	0	0.5235	-0.0454	0.0817	0.6196	-0.0828	0.3976	94.3579	92.232	-2.3797
14	0.2637	-0.034	-0.1066	0.2357	0.197	-0.0286	-0.0077	0.0393	0.0097	-0.0807	0.0395	-0.0221	-0.0255	-0.0826	-0	0.4796	0.0131	-0.0058	0.5562	-0.033	0.3162	84.9775	98.3861	10.8827
15	0.218	-0.2138	-0.1721	0.0974	0.1061	0.4971	-0.1354	-0.1925	-0.3441	0.1538	0.1021	0.182	0.0164	0.0757	-0.0028	0.5104	-0.0062	-0.0609	0.524	-0.0636	0.3343	98.6482	84.3923	49.9995
16	0.2396	-0.1173	-0.0989	0.5492	-0.1898	0.7543	-0.1144	-0.4747	-0.1107	0.1677	0.1394	-0.2437	-0.1096	0.327	-0.0028	0.5941	0.0328	-0.1434	0.6795	-0.0976	0.1829	98.4289	89.2502	47.71
17	0.3968	-0.4998	0.1585	0.7141	-0.3828	0.3372	0.1552	0.1141	-0.1117	-0.0901	-0.0927	0.0971	0.1361	0.0335	0	0.456	-0.0916	-0.0009	0.4815	-0.0672	0.3651	95.1076	76.081	21.1509
18	0.0988	-0.0556	-0.1458	0.519	0.4727	0.6232	-0.0343	-0.027	-0.0199	0.0154	-0.0079	-0.085	-0.0343	-0.0772	-0	0.4771	-0.003	0.0044	0.5038	-0.0904	0.4017	96.1358	83.8421	37.0583
19	0.6211	0.0021	-0.4477	0.0788	-0.0573	0.212	-0.0054	-0.0614	-0.1085	0.1161	-0.1553	-0.179	0.1131	0.1326	-0.0038	0.6062	0.0514	-0.0216	0.4938	0.2698	0.7254	115.4063	-72.7637	55.0603
20	0.7873	-0.0897	-0.1673	0.2497	-0.1746	0.1063	0.0354	0.0043	0.0169	-0.327	0.0821	0.2854	0.0084	-0.0768	0.0001	0.6458	-0.0308	-0.0768	0.4485	0.0867	0.5318	102.114	-52.5027	37.561
21	0.542	0.1616	-0.0829	0.2558	-0.0365	0.004	-0.0393	0.0085	-0.0488	-0.1207	-0.0879	-0.0791	0.113	-0.0086	-0	0.6138	-0.0551	-0.0379	0.4547	0.1178	0.5416	100.034	-56.536	40.8064
22	0.0714	0.1032	0.0364	0.1872	-0.2475	0.3736	-0.072	-0.0524	-0.221	-0.0173	-0.0429	-0.4131	-0.0568	0.1204	-0.0053	0.5109	-0.0068	-0.0302	0.5045	0.2752	0.7184	115.3825	-72.5975	56.0651
23	0.2212	-0.2051	-0.3977	0.6004	-0.1409	0.7502	0.0903	0.1206	-0.089	-0.1601	0.1295	0.0525	-0.0287	-0.1703	0.0132	0.443	-0.0487	-0.2823	0.4189	0.0938	0.5958	77.6104	-62.0148	40.7371
24	0.4254	0.1881	-0.4877	0.1077	-0.0286	0.0681	-0.0067	0.1027	-0.1013	-0.1627	0.0442	0.0152	-0.019	-0.1473	0	0.5496	-0.0414	-0.1832	0.4879	0.1578	0.6444	76.9946	-59.3149	42.9293
25	0.35	0.3365	-0.1183	0.2969	-0.1954	0.2226	0.0742	-0.2061	0.3561	0.1179	-0.1522	0.1039	-0.0422	-0.1625	-0.1892	0.4692	0.0165	0.0022	0.583	0.4808	1.0595	55.2204	-48.4946	63.193
26	0.1702	-0.1001	-0.1934	0.1121	-0.2223	1.023	-0.1949	0.1617	-0.0361	-0.0147	-0.0109	-0.0033	0.1376	-0.4523	-0.2165	0.3487	-0.0612	-0.007	0.6039	0.363	0.8518	59.0576	-50.9162	66.0836
27	0.3056	-0.2445	0.2518	0.172	-0.2103	0.7396	0.3433	-0.2768	0.1085	0.2854	-0.2286	0.189	-0.5055	0.3617	0.0649	0.6047	-0.0219	0.0447	0.5311	0.2423	0.7365	75.4119	-26.0525	64.6661
28	0.3304	0.1592	0.2983	0.0462	0.1207	0.245	0.1311	0.0094	0.2117	0.1136	0.0988	0.1766	-0.0595	-0.1662	0	0.6521	0.0526	0.1626	0.4406	0.2488	0.8281	72.413	-33.7735	68.8906
29	0.2867	0.0404	0.1175	0.0717	0.0956	0.0962	-0.0783	0.1795	-0.0048	-0.0905	0.318	0.0757	0.1309	-0.3682	0	0.52	-0.0486	-0.0375	0.6372	0.2983	0.6124	65.576	-43.6075	47.4536
30	0.2058	0.17	0.1856	0.178	0.1752	0.2711	-0.1143	0.1449	-0.2865	0.0357	0.2451	-0.0229	0.0837	-0.6932	0.0037	0.5508	-0.0544	-0.074	0.7272	0.2154	0.8812	49.9997	-54.7392	72.436
31	0.3752	-0.2652	-0.3948	0.3212	-0.0041	0.4185	0.0809	-0.1892	0.1318	0.2957	0.2072	0.0132	-0.2761	-0.1588	0.0001	0.787	-0.1221	0.1282	0.586	0.0602	0.5581	79.6923	-35.9262	93.3621
32	-0.0052	-0.0708	-0.0693	0.4832	0.1636	0.121	0.3199	0.0791	0.2539	0.051	-0.1075	-0.2141	-0.1286	0.0885	0	0.8007	-0.1281	0.0167	0.5552	0.1621	0.7759	101.8454	-26.0928	75.658
33	0.1634	0.2916	-0.1714	0.0238	-0.4431	0.9496	0.0752	-0.0542	0.0143	0.2917	0.0836	0.0631	-0.2163	-0.0684	0.003	0.7337	-0.1035	0.0804	0.49	0.067	0.589	89.8967	-33.7341	85.3797
34	0.312	0.533	0.131	0.587	-0.0011	-0.0595	0.1329	-0.1433	0.0456	-0.0787	-0.0578	0.0227	0.0439	0.2663	0.0142	0.6054	-0.1675	-0.0871	0.6705	0.2109	0.5493	114.8361	-46.9357	80.0565
35	0.1972	0.1937	0.0575	0.3735	-0.265	0.4483	0.1187	-0.0837	-0.1019	0.0596	-0.1724	-0.0825	-0.0443	0.1987	-0.0001	0.5117	-0.1372	-0.1105	0.5763	0.174	0.5654	116.7184	-48.8675	73.8924
36	0.3058	-0.1614	0.0328	0.1302	0.1897	0.4282	0.0138	-0.0297	-0.2016	-0.6088	-0.1715	-0.0375	-0.0879	-0.0877	0	0.5342	0.0224	0.0193	0.5346	-0.0791	0.7196	158.1323	54.7323	5.6509
37	0.0983	0.1392	0.0949	0.1769	0.1076	0.0277	-0.1896	0.0101	-0.0342	0.0989	0.0263	-0.0988	0.0409	-0.0824	-0	0.4994	0.0826	0.0396	0.5971	-0.0658	0.9323	168.4263	47.9455	9.2198
38	0.288	-0.1593	0.1991	0.2088	0.0946	0.3867	0.1563	0.3488	-0.6803	-0.0883	-0.1496	-0.133	0.3501	0.0186	-0	0.6435	0.0249	-0.2037	0.7468	0.0738	1.1761	182.7312	27.8557	19.6928
39	0.2079	-0.0392	-0.2954	0.2883	0.1405	0.4032	-0.0716	-0.4974	0.031	-0.1423	-0.058	0.0739	-0.0366	0.1323	0.0004	0.382	0.1086	-0.1324	0.8749	0.0518	0.6499	155.357	57.8492	27.3472
40	0.0264	-0.0234	0.0106	0.3202	0.2154	0.1549	0.699	-0.0265	-0.4747	0.0282	0.3788	-0.2408	-0.071	-0.3733	0.0843	0.8321	0.1559	-0.1211	1.0006	0.1117	1.1527	184.6895	35.4691	29.9439
41	0.1263	-0.2312	-0.1864	0.3975	0.3408	0.3211	-0.0701	0.0894	-0.023	-0.2449	0.6678	-1.046	-0.8382	-0.1959	0.066	0.6646	0.1219	-0.0881	0.9047	-0.0446	1.1307	186.5946	41.6667	29.002
42	0.1018	0.1326	-0.04	0.1495	-0.0544	0.0191	-0.1031	0.514	-0.0499	-0.5448	-0.2447	0.1019	0.3291	-0.1301	-0.1686	1.5751	0.0773	0.218	0.9874	-0.4356	0.6473	146.5141	71.6665	-30.0749
43	0.0368	-0.0387	-0.0337	0.0212	0.0286	0.0341	-0.1257	0.1005	0.3422	-0.6136	0.0071	-0.0545	0.4321	-0.2847	-0.0001	1.0846	-0.0571	-0.1451	1.0619	-0.1268	0.5794	145.7695	83.9993	-32.8681
44	0.0085	-0.0092	-0.0065	0.0175	0.0153	0.0112	0.0967	0.3075	0.0025	0.2999	0.1833	0.1643	0.218	-0.0408	-0	1.1066	-0.1252	0.0936	1.1693	-0.2126	0.6579	140.9763	73.1774	-32.8831
45	0.0193	-0.0156	-0.0295	0.011	0.0468	0.1303	0.0959	0.0902	-0.1367	0.1239	0.0407	0.1221	0.1615	0.1441	0.3068	0.9384	0.0446	0.2749	0.9136	-0.3793	0.5444	148.8669	79.0222	-32.6048
46	0.0606	-0.0335	-0.0788	-0.0152	0.0319	0.0441	0.0128	0.7426	0.0199	-0.6467	0.0963	-0.3505	0.3883	0.4388	0.032	1.4457	0.1379	0.3632	1.5827	-0.1494	0.793	172.9463	71.4045	-46.4434
47	0.0213	-0.0089	-0.0263	0.0031	0.0097	0.0316	-0.2928	-0.0524	-0.136	-0.1408	-0.0202	-0.1901	0.3065	-0.2668	-0	1.7126	0.5004	0.4325	1.2889	0.2857	1.5583	174.3964	60.728	-43.551
48	0.0214	0.0175	-0.0146	0.0102	-0.0142	0.0099	-0.4365	0.2296	-0.1196	0.2031	-0.0579	-0.2693	0.3673	-0.0251	-0.0003	1.6726	0.0176	0.2515	1.4366	-0.0147	1.1832	166.7936	75.2327	-46.8532
49	0.2861	-0.1831	-0.0673	0.1608	-0.0451	0.1846	-0.0473	0.0677	-0.132	0.0889	0.1356	-0.3131	0.3298	0.1411	0.3101	1.6044	0.5894	0.9425	2.1919	-0.5482	1.8175	183.7094	66.4235	-42.4669
50	0.0104	-0.0015	0.0085	-0.0058	-0.0125	0.0111	0.0909	0.0176	-0.0851	-0.0619	0.0727	-0.2493	0.1012	0.2141	0	1.973	0.1965	0.1689	1.7416	0.0449	1.7527	181.5617	58.8119	-47.4662
51	0.0111	0.0063	0.0102	0.0135	0.0236	0.0365	0.0124	0.0435	-0.1424	-0.2876	-0.4988	0.1594	-0.0366	-0.1562	-0.0001	1.7072	0.578	0.3974	1.8559	-0.1842	1.2207	178.1992	59.6269	-54.2594
52	1.6182	-0.1463	1.1302	0.7992	0.3335	1.02	-0.1521	-0.1804	0.0096	-0.4377	-0.4393	-0.2541	-0.0287	0.4259	-0.3137	0.6589	0.0762	0.3271	1.0746	0.1445	0.8213	167.7034	92.5349	-22.2597
53	0.0247	-0.0058	0.0205	0.0214	0.0275	0.0609	0.4292	0.3002	0.362	0.2205	-0.3189	0.1438	-0.0744	0.4636	-0.0001	0.6212	0.0071	0.2117	0.9737	-0.052	1.0737	156.1793	92.9362	-19.8756
54	0.1323	0.0609	-0.1322	0.3105	-0.1173	0.1697	0.2855	0.131	0.0425	-0.0877	0.1094	-0.1467	-0.0644	0.2487	0.9586	0.5295	0.065	0.5155	0.9017	-0.3842	0.7081	159.7315	80.4275	-22.9046
55	0.0472	-0.0256	0.0188	0.0411	-0.0627	0.0734	0.2928	0.1106	-0.1003	0.0089	-0.4548	-0.2558	0.3722	0.1143	-0	0.5668	0.0465	0.1196	0.9215	-0.0031	0.7353	160.7897	88.1744	-14.9104
56	0.0116	0.0054	0.0075	0.0331	0.0511	0.0712	0.3271	-0.0522	0.033	-0.3785	-0.5075	-0.0228	0.1793	0.0709	-0.0001	0.6534	0.0744	0.1524	1.0823	-0.0509	0.743	149.2003	89.2167	-21.3778
57	0.0677	0.0815	-0.0224	0.0931	-0.0293	0.018	-0.0318	-0.0986	-0.1447	-0.0764	-0.1956	-0.1362	-0.0033	0.0047	-0.0899	0.948	0.5202	0.4377	1.7506	0.0463	1.2655	184.7624	81.7441	-34.3575
58	0.0734	0.0351	0.0169	0.1286	-0.0655	0.0504	0.1194	0.0527	-0.0951	-0.0056	-0.3512	0.0826	-0.1719	-0.1172	-0.0954	1.7649	-0.0924	1.173	2.1652	-0.2253	1.3537	182.3224	70.6242	-60.7992
59	0.0271	-0.0151	-0.0186	0.0064	0.007	0.0096	-0.2153	0.0337	0.3858	-0.5914	0.439	-0.0047	-0.1036	0.1642	-0.0003	1.2702	0.5203	0.3473	2.075	0.1682	0.9897	180.7366	83.7957	-42.0919
60	0.0592	0.0478	0.0282	0.0517	-0.0066	0.0341	-0.7731	-0.0923	-0.1426	-0.4739	0.279	-0.172	-0.179	0.4436	-0.0005	1.3424	0.1385	0.2387	2.0453	-0.0988	1.0072	176.9759	79.9871	-44.2617
61	0.0054	0.0019	-0.0009	0.0031	-0.0059	0.0094	0.0081	-0.0633	0.1394	-0.0655	0.1446	-0.0819	-0.0651	-0.1532	-0	2.0206	0.1166	0.8672	1.8242	0.0696	1.3342	183.1898	80.0246	-63.3159
62	0.0081	-0.0147	-0.0066	0.0123	0.0079	0.0045	-0.041	-0.1958	0.136	-0.206	0.1486	0.2532	-0.031	-0.199	-0.0002	1.4358	0.0068	0.2026	2.0031	0.1106	1.2271	186.3161	88.0399	-44.7949
63	0.3647	0.5283	-0.0772	0.924	-0.2074	0.0738	-0.4412	-0.0078	-0.319	0.157	-0.0024	-0.1181	0.7909	-0.3437	-0.1589	0.7682	-0.1941	0.08	0.76	-0.2838	0.6336	104.018	63.3403	-44.6795
64	0.0478	0.066	0.0244	0.3808	0.1059	0.144	-0.0047	0.2056	-0.0267	0.3228	0.1852	0.3157	0.5943	-0.4011	0	0.9212	-0.2591	0.1271	0.7411	-0.0575	0.5875	102.6849	70.2556	-35.1995
65	0.1086	-0.0608	0.1341	0.0568	0.0878	0.4435	-0.0982	0.5017	-0.1101	0.1363	0.0669	-0.0305	0.3232	-0.0527	-1.1045	0.7244	-0.564	-0.0135	1.0477	-0.6772	0.0212	104.1885	67.894	-62.7575
66	0.3893	-0.1631	-0.0641	0.0958	0.0037	0.2707	0.2182	0.252	-0.3636	-0.1215	0.0756	-0.1737	0.4074	-0.1036	0.8098	1.3233	-0.4842	-0.1244	1.5934	-0.348	0.662	105.76	65.7233	-75.6669
67	0.753	0.0953	-0.6143	1.0208	-0.6309	1.5245	-0.3267	-0.2425	-0.0927	-0.1979	-0.0819	-0.1155	0.1882	-0.4934	-1.8428	0.668	-0.0996	-0.0094	0.8188	-0.1381	0.207	108.4113	89.8459	-42.2105
68	0.3104	0.0262	0.017	0.1176	0.1082	0.5718	-0.0623	0.3117	-0.2196	0.0574	0.1779	0.0508	-0.0744	-0.0697	0.1219	0.6907	-0.104	0.0668	0.9927	-0.7294	-0.1209	108.0529	89.4401	-46.2664
69	0.2249	-0.0452	0.2819	0.0204	-0.0345	0.3704	0.2771	-0.015	-0.1547	-0.0163	0.1473	0.0745	0.4735	-0.2508	0.4868	0.6653	-0.2147	-0.4307	1.7291	-0.7874	0.3863	97.4865	76.8908	-74.8406
70	-0.0039	0.033	0.0131	0.0545	0.1055	0.1471	0.0545	0.115	-0.0933	0.1917	-0.1947	0.1073	-0.291	-0.7983	-0.0855	0.9556	-0.099	-0.1276	1.7175	-0.2111	0.4654	95.828	80.0834	-73.8096
71	0.3936	-0.0068	0.25	0.4495	-0.077	0.1523	-0.467	0.3144	-0.254	-0.6223	0.1599	-0.5669	0.6001	0.2956	-0.1146	0.9419	-0.0765	0.31	0.5615	-0.3679	0.8269	87.6055	43.6609	-10.0505
72	0.3749	0.2041	-0.3852	0.2823	-0.2249	0.5212	0.0576	0.1171	-0.3177	-0.4134	0.0438	0.1549	0.329	0.337	-0.184	1.0824	-0.0396	-0.0363	0.4582	-0.1452	0.991	82.9341	38.0172	-9.3162
73	0.193	0.4255	-0.4878	1.336	-0.46	2.3273	0.1075	0.5972	0.1233	-0.5294	0.9744	-0.0144	-0.0267	-0.1141	0.8834	2.6381	-0.6883	-0.2973	1.3538	-0.0932	1.2815	66.0875	24.4437	-31.8822
74	0.1717	0.0766	0.0592	0.2997	-0.2536	0.2844	-0.1725	0.1049	0.0214	-0.1065	0.1533	0.1674	0.1467	0.0289	-0.0005	0.6492	-0.2456	-0.0319	0.7206	-0.153	0.4034	74.2256	59.7809	-11.2395
75	0.2105	0.0873	0.195	0.1289	0.2204	0.7034	0.0336	0.0419	-0.0778	0.0324	-0.0753	0.0359	0.2331	0.129	-0.447	1.4464	-0.2933	-0.8032	1.1755	0.0638	0.8618	55.4112	29.4609	-27.296
76	0.1023	-0.1026	0.0631	0.1365	-0.1231	0.1574	0.1344	-0.0209	-0.3155	-0.023	0.3051	-0.0246	0.3416	-0.0287	0.5507	2.0301	-0.9267	-0.8526	1.4545	-0.0157	1.1375	55.7651	30.3865	-21.0683
77	-0.0018	0.003	0.001	0.0161	0.0157	0.0072	-0.5218	0.1567	0.142	0.025	-0.2675	-0.138	0.101	0.0896	0.0006	2.0961	-0.0982	-0.5516	1.9174	0.2197	2.098	47.5505	36.1397	-21.3341
78	0.0803	0.0919	-0.0635	0.0953	-0.0731	0.0378	-0.2184	-0.0102	0.11	-0.063	0.0934	0.1853	0.4156	0.6754	0	0.9603	0.3144	-0.0677	1.0723	-0.4502	1.3067	123.0508	26.3404	15.6579
79	0.6504	0.0875	0.6977	0.0128	0.0854	0.7291	0.7687	0.0922	-0.4055	-0.7362	0.1263	-0.6753	0.2551	0.429	0.2229	0.7524	0.2343	0.1436	0.6415	-0.1229	1.1945	118.4722	37.1359	18.5833
80	0.0407	0.0522	-0.029	0.0449	-0.0327	0.0163	0.1042	0.0304	-0.0684	-0.4306	0.3941	-0.5045	-0.0589	0.2696	-0	0.7505	0.3181	0.2196	0.9279	-0.0099	1.1989	126.8963	38.5283	19.4809
81	0.1476	-0.1685	0.098	0.2004	-0.1632	0.1655	-0.0366	0.2094	0.0164	0.2209	-0.0024	-0.4506	-0.0428	0.1625	0.009	0.7827	0.3511	0.0093	0.6737	0.1838	1.1853	120.7163	32.985	21.5431
82	0.0258	0.045	-0.0917	0.0753	-0.199	0.5172	-0.2556	-0.1231	-0.1108	-0.2067	0.091	0.0167	0.661	0.2528	-0.2545	0.9742	0.1995	-0.3975	0.4474	-0.0897	1.6698	119.4582	10.4726	21.8899
83	0.0241	-0.0181	-0.0012	0.019	-0.0068	0.0009	-0.1145	0.0214	0.1419	-0.3447	-0.7711	-0.3031	0.3426	-0.3392	0.0005	2.058	0.3558	0.2006	1.2293	-0.2865	1.943	106.7395	-2.4578	27.1726
84	-0.001	0.0017	0.0013	0.0223	0.0038	-0.0045	0.2148	0.4193	0.0732	-0.4091	-0.1647	-0.1207	0.4412	-0.3037	0.0001	2.0638	0.2667	-0.1844	1.5366	-0.3042	1.5588	110.0541	1.6797	13.9035
85	0.0075	-0.0078	0.0334	0.0111	-0.0369	0.0692	0.187	-0.0859	-0.0706	-0.2654	-0.0979	-0.0221	-0.2365	-0.8126	0.0009	1.895	0.3528	-0.4703	1.0214	-0.1304	1.7603	110.4027	3.8766	23.6683
86	0.045	-0.0031	0.0699	-0.0027	-0.0106	0.1123	0.4362	0.1457	-0.2666	-0.1096	0.0906	0.1794	0.13	0.3395	0.0556	2.6757	0.4061	0.0196	1.0281	-0.8318	2.2366	107.8424	-6.2608	15.427
87	0.0021	0.0116	-0.0033	0.0075	-0.0151	0.0014	0.3951	0.125	-0.1863	-0.0861	0.2428	0.067	0.103	0.1848	0.0006	2.1607	0.1958	-0.2024	0.9566	-0.4372	2.1363	115.6837	-6.8297	15.56
88	0.003	-0.01	-0.0037	0.0275	0.0266	0.0104	0.081	0.0201	-0.2971	0.1046	-0.1334	0.2906	0.3528	-0.3355	-0.0004	0.8947	-0.0092	-0.1419	0.7167	0.288	1.2715	100.7521	31.4535	36.8456
89	0.1172	-0.1188	0.1961	0.1234	-0.1844	0.3404	0.0666	-0.1757	-0.3296	-0.074	0.0354	-0.3466	-0.0805	-0.3923	0.0026	0.5959	-0.0292	0.0536	0.3985	0.1038	0.9666	105.411	40.183	29.5254
90	0.204	-0.0352	-0.0856	0.2298	-0.1173	0.0922	0.1871	0.1729	0.169	0.3041	-0.0185	0.0315	-0.1743	0.1169	0.0001	0.6125	-0.0039	0.032	0.4979	-0.0401	1.136	102.5002	40.2093	29.0923
91	0.4093	-0.0337	-0.4714	0.0047	0.0326	0.5418	0.3805	-0.1446	-0.0284	-0.1436	0.339	-0.0983	-0.3089	-0.0156	0.0494	0.6594	-0.0118	-0.2403	0.623	0.052	1.245	111.8227	36.1527	31.8312
92	0.1321	-0.0317	0.0224	0.0491	0.0084	0.0132	-0.1605	-0.0774	-0.0919	-0.1431	0.2315	-0.1979	0.0415	0.0709	0.0004	1.0719	0.0186	0.0108	0.5325	-0.0917	1.9732	100.3576	4.2183	33.31
93	0.004	-0.007	0.0009	0.0101	-0.0018	-0.0003	0.0082	0.0478	-0.019	-0.0315	-0.1075	-0.0891	0.0787	0.0239	-0.0001	1.9823	0.677	-0.2439	1.2343	0.3076	2.2135	120.2855	-9.1273	29.3812
94	0.0235	0.0113	-0.0273	0.0002	-0.027	0.0747	-0.1088	-0.1487	-0.2275	0.0509	-0.0604	0.0713	0.3332	0.2098	0	1.8513	0.1431	-0.1541	0.8754	0.0506	1.6896	108.7652	7.3382	34.856
95	0.0004	0.0095	0.0194	0.2604	0.1108	0.0506	0.2304	-0.1497	0.3644	0.4894	0.2688	0.0669	-0.1798	0.04	0.3604	2.0202	0.4096	-0.1797	0.7937	0.4686	1.5719	110.352	-0.1853	39.0031
96	0.2219	0.0786	-0.0022	0.6946	0.0807	0.0035	0.1641	0.1242	0.1118	0.3756	0.1938	0.4991	-0.7917	0.1778	0.039	0.9428	-0.161	0.1237	0.6476	0.1095	0.6168	135.1197	-17.7156	22.8443
97	0.1632	0.1751	-0.0553	0.1918	-0.1177	0.1471	-0.2054	-0.2109	-0.0805	0.0596	-0.0811	0.378	-0.7822	0.0219	-0.0003	0.805	-0.1506	0.0663	0.7369	0.0211	0.6976	140.5521	-22.4435	28.1801
98	0.2583	0.0879	-0.0902	0.0352	-0.0813	0.0581	-0.0491	0.4105	-0.1172	-0.1429	0.0005	0.1549	-0.4023	0.225	0	0.9877	-0.3475	0.0485	0.9861	-0.0233	0.7906	156.6858	-18.5766	-0.286
99	0.1547	-0.106	-0.0672	0.1998	-0.0124	0.044	0.1927	0.3682	0.0956	-0.1058	0.4893	0.112	-0.5817	-0.3347	0.0002	1.2918	-0.3258	0.2267	0.9606	0.0546	0.7617	159.1878	-17.4698	-5.0149
100	0.0207	-0.0006	0.0097	0.0001	-0.0063	0.0142	0.1981	-0.0745	-0.3945	-0.1423	-0.2855	0.0171	0.047	0.1407	-0.0003	0.816	0.113	0.0347	0.7613	-0.0806	1.087	143.3325	-45.7709	15.0965
101	0.1153	-0.0236	-0.0142	0.0939	0.0784	0.1481	-0.2757	-0.6331	-0.2021	-0.0197	-0.1846	-0.0643	-0.3681	0.7569	-0.0056	0.6139	0.0561	0.0937	0.5612	0.0645	0.9287	137.5533	-42.8202	24.7096
102	0.0604	0.1015	-0.1482	0.4385	0.083	0.7525	0.1476	0.0319	-0.1598	-0.4494	0.5745	0.2209	-0.4461	-0.0293	0.0791	0.6044	-0.0761	-0.0467	0.3019	0.0763	0.4661	136.5791	-33.1343	15.529
103	0.0986	0.0569	-0.0002	0.0339	0.0377	0.0769	-0.5753	0.0052	-0.5772	-0.4437	-0.1443	-0.04	0.1518	-0.2685	-0.0033	0.7138	0.0441	0.0936	0.516	0.0854	0.826	130.3625	-45.433	20.174
104	0.1159	-0.0167	-0.0404	0.0281	0.0348	0.0432	-0.37	-0.2823	-0.2898	-0.3308	-0.0832	-0.0858	-0.1117	-0.3735	0.0002	0.9019	-0.1352	-0.0262	0.635	-0.0439	0.5922	138.6009	-39.4802	12.9635
105	0.2845	0.039	0.2177	0.14	0.0168	0.1639	0.0476	0.2176	-0.5565	-0.0319	-0.235	-0.0812	-0.3438	-0.5055	-0.0001	0.7055	-0.1102	0.0624	0.6952	0.0839	0.6271	142.8045	-37.4394	16.6498
106	0.0241	-0.0024	-0.0285	0.0008	0.0087	0.0267	-0.2912	0.0953	0.5197	0.1908	0.1841	-0.3135	0.7712	0.0474	-0.0003	1.1367	-0.1207	0.0723	0.7892	-0.2922	1.2294	169.2391	-23.3417	-3.7254
107	0.0143	-0.0042	-0.0015	0.0043	0.0174	0.0399	-0.2991	-0.0945	0.0442	0.6685	0.4799	-0.3462	0.0987	0.2619	-0	1.1963	-0.1341	0.0375	1.0058	-0.2164	0.9743	165.8732	-34.5921	6.4546
108	0.005	0.0015	0.0302	0.0103	-0.0104	0.0679	-0.1715	-0.0121	0.2592	0.1259	0.2366	-0.5678	-0.1303	-0.0379	-0	0.7203	-0.2069	0.1248	0.8256	-0.1045	1.0918	166.2158	-27.5753	1.1886
109	0.002	0.0102	-0.0127	0.0034	-0.0117	0.0161	-0.0385	-0.0929	0.0547	0.2071	0.1709	-0.2093	0.1753	0.2939	-0.0001	1.2427	-0.3228	0.3455	1.3938	-0.1839	1.3167	168.3317	-38.8367	-0.8
110	0.0877	0.0209	-0.1134	0.1245	0.1918	0.5425	0.042	-0.0241	0.0468	-0.0947	-0.0196	0.0183	-0.047	0.0006	0.3095	1.1882	0.1634	-0.209	1.2888	-0.2442	0.3047	80.4429	-41.1444	-6.5126
111	0.1649	0.0863	-0.0203	0.1634	-0.0882	0.0309	0.3806	0.5678	0.2937	0.3718	-0.0936	-0.4256	0.3767	0.1522	0.0099	0.9262	0.2005	-0.2326	1.0603	0.2465	0.5845	84.7875	-36.7401	4.3354
112	0.1689	0.0567	-0.0469	0.0258	-0.0023	0.0424	0.3335	0.1433	0.5192	0.0432	-0.2778	0.0355	0.3926	0.1595	0.0025	0.9807	0.0845	-0.1467	0.8298	0.2089	0.5822	84.5884	-38.1661	3.441
113	0.2505	-0.1827	0.0252	0.4672	0.3844	0.5376	0.3307	-0.0131	-0.0532	-0.2818	0.1483	-0.0795	-0.091	0.3773	0.4766	1.5035	-0.0271	-0.2012	1.9659	0.1406	0.3919	86.9843	-24.2455	-34.3074
114	0.0383	0.0436	-0.008	0.1768	0.048	0.0286	0.079	0.1431	-0.1463	-0.2798	0.077	-0.2783	0.2839	-0.0188	-0.0004	2.0321	0.3703	-0.1643	2.2418	0.2268	0.8607	85.6795	-31.6515	-29.1444
115	0.0026	0.0066	-0.002	0.006	0.0016	0.0099	0.1709	0.0264	-0.306	-0.0887	0.288	0.0948	0.0246	-0.0881	-0.0001	1.803	0.2753	0.5323	1.809	0.0649	0.9642	97.8706	-31.6999	-32.628
116	0.2127	0.166	-0.0075	0.3401	0.1074	0.0604	-0.0063	0.2194	0.0079	-0.4866	-0.0912	-0.2036	0.2111	0.3495	0.0536	1.5358	0.5601	0.0062	2.2664	0.7085	0.9298	84.8413	-25.3035	-24.6297
117	0.0041	-0	-0.0039	0.0014	0.001	0.0079	0.2667	-0.1565	-0.1111	-0.0968	0.0837	0.0671	-0.0538	-0.0514	-0.0001	2.6511	0.619	0.0768	2.4194	0.3838	0.8812	99.4924	-27.2678	-45.7162
118	0.0164	-0.0624	-0.0245	0.2047	0.0692	0.0217	0.204	-0.2784	-0.2319	0.0134	0.2907	-0.1737	0.0112	0.0697	-0.0065	1.82	0.4998	-0.3545	2.6784	-0.1438	0.6959	83.6922	-35.6073	-35.5938
119	0.7804	-0.0104	0.0951	0.0986	0.173	0.2851	-0.2432	-0.1857	0.3115	-0.2251	0.0451	-0.4907	-0.1112	0.0187	-0.0603	1.0149	0.0226	-0.0987	0.5356	0.3897	0.8309	66.36	3.3132	15.4385
120	0.1534	0.0773	0.5339	0.0351	0.1931	1.8212	-0.1181	0.2323	-0.6614	-0.0669	0.1312	0.2566	0.005	0.4962	0.1384	0.6125	0.0801	-0.0398	0.4704	0.2505	1.0219	68.8192	-6.4239	21.9286
121	0.0855	-0.0787	-0.1325	0.0685	0.1419	0.2508	0.3737	0.0389	0.0238	-0.1491	0.3611	0.1849	0.1601	0.2772	0.0757	0.7025	0.0661	-0.1638	0.7543	0.2793	0.8771	64.128	-3.8861	20.6449
122	0.0309	-0.0424	-0.0225	0.1207	0.0796	0.043	0.1246	0.0672	0.0608	-0.1904	-0.0984	-0.0679	0.0966	-0.4055	0	1.0581	0.197	-0.0549	0.6653	0.165	0.8294	51.9468	11.9739	10.442
123	0.0031	-0.0498	0.0395	0.1572	-0.1712	0.172	-0.2562	-0.3252	0.3055	0.1986	0.0617	0.2384	-0.1325	0.2776	-0	0.8544	0.1022	-0.1267	0.8642	0.0024	0.8256	45.1504	25.1193	14.1708
124	0.0009	-0.0109	0.0091	0.0184	-0.0155	0.0155	-0.0703	0.3584	-0.2451	0.1883	0.2008	0.1325	-0.2403	-0.1746	0.0001	0.8946	0.1374	-0.0432	0.6833	-0.085	0.8594	48.6066	10.4174	11.3246
125	0.1517	-0.0286	0.0327	0.0692	-0.0878	0.0793	0.0798	-0.278	-0.1686	0.0136	0.0217	-0.2698	-0.0078	-0.1866	0.0001	0.8637	0.217	-0.1062	0.8146	0.1542	1.0015	43.9147	29.5981	11.3938
126	0.484	0.0008	0.2581	0.2203	-0.1141	0.2133	0.2374	-0.143	-0.1498	-0.0433	-0.0007	0.0126	-0.0678	0.1278	0	0.7031	0.0863	0.0654	0.6644	0.2731	0.929	52.4343	-4.369	36.7154
127	0.6988	-0.5939	-0.583	0.6465	0.1171	1.261	-0.3361	0.2797	-0.5108	-0.2694	0.1769	0.9369	0.2468	0.1106	-0.5693	0.5912	0.0277	-0.4281	0.7197	0.1324	1.2156	34.4451	16.0322	12.7651
128	0.1912	-0.3454	-0.2402	0.5854	0.4111	0.2685	-0.4124	0.3516	-0.3101	0.0826	0.096	0.5365	0.7121	-0.3456	-0.0568	0.6621	-0.0747	-0.2083	0.9667	0.0901	1.1686	32.3969	12.8345	14.1042
129	0.0271	-0.0433	-0.0467	0.0509	0.0608	0.0694	0.0804	0.2067	-0.1011	-0.0634	-0.129	-0.2235	-0.3677	-0.1891	-0.0099	1.8846	0.0353	0.5603	0.9098	0.4106	2.1697	85.1573	25.286	55.2026
130	0.0139	-0.0072	0.0544	0.1098	0.0494	0.2897	-0.2145	0.2358	0.6755	-0.093	0.0573	0.084	0.2438	-0.1435	0.0924	1.1207	0.0023	0.3457	1.201	0.7045	1.7506	92.1866	10.6937	54.3264
131	0.2175	0.0942	-0.0201	0.0398	-0.0085	0.0136	0.1292	-0.0038	0.2534	-0.304	-0.2674	-0.0747	0.3232	0.2357	-0.0225	1.0132	0.1653	0.3117	0.6537	0.2533	2.6982	80.8471	17.7057	64.8516
132	-0.0001	0.0038	-0.0112	0.0057	-0.0128	0.0115	-0.1743	-0.1218	0.47	-0.3193	-0.3742	0.477	-0.3625	0.1474	0	1.3195	0.1687	-0.0358	1.0262	0.2893	1.6036	80.074	12.8395	54.4327
133	0.2103	-0.1379	0.0474	0.1095	-0.0672	0.0696	-0.1316	-0.0175	0.2486	-0.1658	-0.1205	0.0761	-0.0456	0.1091	-0.0993	1.5662	0.2957	0.2642	0.4416	0.4114	2.3192	83.0992	18.1643	60.5214
134	0.1454	-0.059	0.0981	0.03	-0.0531	0.0687	0.1339	-0.7621	-0.2924	0.4636	-0.1716	-0.3922	-1.1061	-0.4415	-0.0054	1.7861	0.3343	0.2487	1.1423	0.1748	2.4055	82.6732	40.5107	64.4538
135	0.0626	-0.0497	0.046	0.0257	-0.0286	0.015	0.0134	-0.456	0.3713	-1.0783	0.2127	-0.3568	-0.0634	-0.0156	-0.0001	1.8561	0.1299	-0.1879	1.7785	-0.2517	3.1525	91.3777	51.3668	71.3723
136	0.0065	0.0367	-0.0253	0.1316	-0.1527	0.138	0.36	0.0432	0.2439	-0.2342	0.2181	-0.1908	0.0994	-0.0512	-0.0001	1.7255	0.1293	0.016	1.1749	-0.1215	2.8545	87.7862	46.8236	72.4297
137	0.0078	0.0017	0.0186	0.0012	0.0072	0.0192	0.1541	0.0708	-0.0961	-0.3596	-0.0267	0.2154	0.1932	-0.0308	-0.0001	2.5256	-0.3819	-0.361	2.2682	0.2444	2.3466	94.0904	57.9702	68.9509
138	-0.007	-0.0005	-0.0052	-0.0001	0.0104	0.0137	0.2625	0.0336	-0.0808	-0.2023	0.1392	0.3098	0.0377	-0.0713	0	2.6125	-0.5659	0.2711	1.6742	-0.3871	2.5201	87.4977	58.7385	64.3519
139	-0.0009	-0.0616	0.0803	0.178	0.0426	0.4618	0.0086	0.0464	0.1712	-0.1566	-0.2704	-0.113	0.0497	-0.0039	-0.5343	1.2824	-0.0244	0.6346	0.1751	-0.467	1.9467	89.5334	15.2163	78.7784
140	0.2871	0.0963	-0.3911	0.5953	-0.9433	1.7148	0.885	0.1894	0.4748	0.7901	1.0551	-0.0738	0.3094	-0.1382	0.2926	2.4706	0.4753	-0.2625	1.5067	-0.6138	3.2662	79.5906	46.865	82.3825
141	0.0533	-0.0903	0.1651	0.1953	-0.2394	0.3686	0.1863	-0.0535	0.6162	-0.2754	0.2349	0.0571	-0.3546	0.2021	0.1553	1.3198	-0.4488	0.0934	0.7	0.031	1.315	131.3205	9.151	61.3383
142	0.0531	-0.1124	-0.0146	0.1347	0.0298	-0.0034	-0.0679	0.4242	0.4664	0.2555	-0.0079	-0.4593	0.142	0.3986	0	1.7004	-0.5732	0.1911	1.8983	-0.0194	2.4881	160.2684	24.0882	57.6421
143	0.3787	0.1864	-0.2318	0.2821	0.1055	0.3885	0.677	-0.2294	0.6101	0.0299	0.1481	0.1495	-0.1422	0.2839	1.44	0.238	-1.2726	-0.0378	0.3636	-0.3668	0.7165	139.6728	-10.602	67.0225
144	0.2444	0.1109	-0.385	0.0397	-0.1522	0.6043	-0.4778	-0.0623	-0.1495	0.1765	-0.2455	-0.3776	-0.2338	0.1224	-0.7455	1.0922	-0.8651	-0.0944	1.3611	-0.4232	2.0653	163.0831	17.1934	72.2477
145	0.0225	0.0418	0.0525	0.052	0.0943	0.112	0.2022	0.3001	0.1259	0.3071	0.3142	-0.294	-0.0343	0.5344	0.003	1.1639	0.0023	0.1066	1.0671	0.3168	1.2933	103.7591	-20.5006	-4.1988
146	0.024	-0.0069	-0.0114	0.0845	-0.0734	0.0602	0.4224	0.2239	-0.0913	0.3605	-0.6068	-0.1499	-0.0234	0.3405	0.0003	1.0385	-0.0643	-0.1118	0.9769	0.1604	0.5256	98.9436	-26.8961	4.8265
147	0.0213	0.023	-0.0217	0.0181	-0.0205	0.0426	0.0923	0.6282	0.0154	0.078	0.1215	-0.1065	-0.1376	0.5946	-0.0001	0.7791	-0.1206	-0.1517	1.1526	0.1172	0.8994	107.3316	-28.6062	5.7997
148	0.0368	-0.0073	0.0265	0.0101	0.0011	0.0161	-0.2797	0.3568	0.261	0.1705	0.2531	-0.0403	-0.2049	0.0777	-0	0.968	-0.0303	0.0506	1.0366	0.2285	0.5666	101.0135	-28.2823	-0.0305
149	0.0318	-0.0186	-0.0298	0.0492	-0.04	0.2294	0.0599	0.1877	0.2726	-0.0362	0.1608	0.0566	0.1323	0.0373	0.6991	0.757	0.2547	0.3141	1.3843	0.6551	0.441	99.9991	-19.9112	-20.5565
150	0.0827	0.0618	-0.0806	0.0374	-0.0747	0.0947	0.2282	0.1002	0.5585	0.1118	0.3303	0.3707	-0.2597	-0.1742	-0.0002	1.8623	0.0465	0.2329	1.8689	0.4001	1.4576	89.5055	-13.2317	-29.0962
151	0.0387	0.0882	-0.0594	0.1898	-0.1238	0.0804	0.055	-0.0984	-0.0076	0.0544	0.1239	0.0422	0.0225	0.3161	0.0555	1.3482	-0.1027	-0.1591	1.9715	0.3538	0.5032	99.0605	-23.3923	-22.5296
152	0.0487	0.0393	-0.0337	0.0444	-0.0263	0.0206	-0.0785	0.0782	0.0994	-0.0607	-0.1049	0.0313	-0.0104	-0.3233	-0.1933	2.3178	0.2065	-0.1968	2.3419	0.5321	0.6153	84.1374	-13.9777	-31.0887
153	0.0146	0.009	0.0177	0.0082	0.0122	0.006	0.0577	-0.2492	0.0558	0.0837	0.2141	-0.1784	0.1203	0.482	-0.0002	2.3602	0.1215	-0.5046	1.8239	0.4855	1.6326	89.321	-6.3695	-32.5871
154	0.0214	-0.0095	-0.0239	-0.0063	0.0108	0.025	0.2366	-0.0452	0.1783	-0.0318	0.3516	0.015	-0.0499	0.0066	-0.0002	1.8443	0.0072	0.1822	1.8172	-0.0416	1.0731	97.4648	-7.0884	-33.5159

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 20 through 181 )
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 182 through 473 )
3	X-RAY DIFFRACTION	3	chain 'B' and (resid 20 through 56 )
4	X-RAY DIFFRACTION	4	chain 'B' and (resid 57 through 125 )
5	X-RAY DIFFRACTION	5	chain 'B' and (resid 126 through 181 )
6	X-RAY DIFFRACTION	6	chain 'B' and (resid 182 through 335 )
7	X-RAY DIFFRACTION	7	chain 'B' and (resid 336 through 473 )
8	X-RAY DIFFRACTION	8	chain 'C' and (resid 20 through 125 )
9	X-RAY DIFFRACTION	9	chain 'C' and (resid 126 through 181 )
10	X-RAY DIFFRACTION	10	chain 'C' and (resid 182 through 312 )
11	X-RAY DIFFRACTION	11	chain 'C' and (resid 313 through 473 )
12	X-RAY DIFFRACTION	12	chain 'D' and (resid 20 through 125 )
13	X-RAY DIFFRACTION	13	chain 'D' and (resid 126 through 181 )
14	X-RAY DIFFRACTION	14	chain 'D' and (resid 182 through 473 )
15	X-RAY DIFFRACTION	15	chain 'E' and (resid 20 through 86 )
16	X-RAY DIFFRACTION	16	chain 'E' and (resid 87 through 125 )
17	X-RAY DIFFRACTION	17	chain 'E' and (resid 126 through 181 )
18	X-RAY DIFFRACTION	18	chain 'E' and (resid 182 through 473 )
19	X-RAY DIFFRACTION	19	chain 'F' and (resid 20 through 125 )
20	X-RAY DIFFRACTION	20	chain 'F' and (resid 126 through 181 )
21	X-RAY DIFFRACTION	21	chain 'F' and (resid 182 through 312 )
22	X-RAY DIFFRACTION	22	chain 'F' and (resid 313 through 473 )
23	X-RAY DIFFRACTION	23	chain 'G' and (resid 20 through 181 )
24	X-RAY DIFFRACTION	24	chain 'G' and (resid 182 through 473 )
25	X-RAY DIFFRACTION	25	chain 'H' and (resid 20 through 86 )
26	X-RAY DIFFRACTION	26	chain 'H' and (resid 87 through 125 )
27	X-RAY DIFFRACTION	27	chain 'H' and (resid 126 through 181 )
28	X-RAY DIFFRACTION	28	chain 'H' and (resid 182 through 335 )
29	X-RAY DIFFRACTION	29	chain 'H' and (resid 336 through 383 )
30	X-RAY DIFFRACTION	30	chain 'H' and (resid 384 through 473 )
31	X-RAY DIFFRACTION	31	chain 'I' and (resid 20 through 125 )
32	X-RAY DIFFRACTION	32	chain 'I' and (resid 126 through 181 )
33	X-RAY DIFFRACTION	33	chain 'I' and (resid 182 through 473 )
34	X-RAY DIFFRACTION	34	chain 'J' and (resid 20 through 160 )
35	X-RAY DIFFRACTION	35	chain 'J' and (resid 161 through 473 )
36	X-RAY DIFFRACTION	36	chain 'K' and (resid 1 through 80 )
37	X-RAY DIFFRACTION	37	chain 'K' and (resid 81 through 123 )
38	X-RAY DIFFRACTION	38	chain 'K' and (resid 124 through 217 )
39	X-RAY DIFFRACTION	39	chain 'L' and (resid 2 through 119 )
40	X-RAY DIFFRACTION	40	chain 'L' and (resid 120 through 145 )
41	X-RAY DIFFRACTION	41	chain 'L' and (resid 146 through 223 )
42	X-RAY DIFFRACTION	42	chain 'O' and (resid 1 through 37 )
43	X-RAY DIFFRACTION	43	chain 'O' and (resid 38 through 66 )
44	X-RAY DIFFRACTION	44	chain 'O' and (resid 67 through 80 )
45	X-RAY DIFFRACTION	45	chain 'O' and (resid 81 through 101 )
46	X-RAY DIFFRACTION	46	chain 'O' and (resid 102 through 144 )
47	X-RAY DIFFRACTION	47	chain 'O' and (resid 145 through 163 )
48	X-RAY DIFFRACTION	48	chain 'O' and (resid 164 through 177 )
49	X-RAY DIFFRACTION	49	chain 'O' and (resid 178 through 192 )
50	X-RAY DIFFRACTION	50	chain 'O' and (resid 193 through 203 )
51	X-RAY DIFFRACTION	51	chain 'O' and (resid 204 through 218 )
52	X-RAY DIFFRACTION	52	chain 'P' and (resid 2 through 17 )
53	X-RAY DIFFRACTION	53	chain 'P' and (resid 18 through 40 )
54	X-RAY DIFFRACTION	54	chain 'P' and (resid 41 through 51 )
55	X-RAY DIFFRACTION	55	chain 'P' and (resid 52 through 98 )
56	X-RAY DIFFRACTION	56	chain 'P' and (resid 99 through 119 )
57	X-RAY DIFFRACTION	57	chain 'P' and (resid 120 through 134 )
58	X-RAY DIFFRACTION	58	chain 'P' and (resid 135 through 148 )
59	X-RAY DIFFRACTION	59	chain 'P' and (resid 149 through 165 )
60	X-RAY DIFFRACTION	60	chain 'P' and (resid 166 through 191 )
61	X-RAY DIFFRACTION	61	chain 'P' and (resid 192 through 203 )
62	X-RAY DIFFRACTION	62	chain 'P' and (resid 204 through 220 )
63	X-RAY DIFFRACTION	63	chain 'Q' and (resid 1 through 18 )
64	X-RAY DIFFRACTION	64	chain 'Q' and (resid 19 through 80 )
65	X-RAY DIFFRACTION	65	chain 'Q' and (resid 81 through 163 )
66	X-RAY DIFFRACTION	66	chain 'Q' and (resid 164 through 217 )
67	X-RAY DIFFRACTION	67	chain 'R' and (resid 2 through 64 )
68	X-RAY DIFFRACTION	68	chain 'R' and (resid 65 through 134 )
69	X-RAY DIFFRACTION	69	chain 'R' and (resid 135 through 165 )
70	X-RAY DIFFRACTION	70	chain 'R' and (resid 166 through 222 )
71	X-RAY DIFFRACTION	71	chain 'S' and (resid 1 through 53 )
72	X-RAY DIFFRACTION	72	chain 'S' and (resid 54 through 126 )
73	X-RAY DIFFRACTION	73	chain 'S' and (resid 127 through 218 )
74	X-RAY DIFFRACTION	74	chain 'T' and (resid 2 through 119 )
75	X-RAY DIFFRACTION	75	chain 'T' and (resid 120 through 149 )
76	X-RAY DIFFRACTION	76	chain 'T' and (resid 150 through 203 )
77	X-RAY DIFFRACTION	77	chain 'T' and (resid 204 through 220 )
78	X-RAY DIFFRACTION	78	chain 'M' and (resid 1 through 18 )
79	X-RAY DIFFRACTION	79	chain 'M' and (resid 19 through 53 )
80	X-RAY DIFFRACTION	80	chain 'M' and (resid 54 through 80 )
81	X-RAY DIFFRACTION	81	chain 'M' and (resid 81 through 101 )
82	X-RAY DIFFRACTION	82	chain 'M' and (resid 102 through 126 )
83	X-RAY DIFFRACTION	83	chain 'M' and (resid 127 through 144 )
84	X-RAY DIFFRACTION	84	chain 'M' and (resid 145 through 163 )
85	X-RAY DIFFRACTION	85	chain 'M' and (resid 164 through 192 )
86	X-RAY DIFFRACTION	86	chain 'M' and (resid 193 through 203 )
87	X-RAY DIFFRACTION	87	chain 'M' and (resid 204 through 217 )
88	X-RAY DIFFRACTION	88	chain 'N' and (resid 2 through 17 )
89	X-RAY DIFFRACTION	89	chain 'N' and (resid 18 through 60 )
90	X-RAY DIFFRACTION	90	chain 'N' and (resid 61 through 108 )
91	X-RAY DIFFRACTION	91	chain 'N' and (resid 109 through 119 )
92	X-RAY DIFFRACTION	92	chain 'N' and (resid 120 through 138 )
93	X-RAY DIFFRACTION	93	chain 'N' and (resid 139 through 149 )
94	X-RAY DIFFRACTION	94	chain 'N' and (resid 150 through 183 )
95	X-RAY DIFFRACTION	95	chain 'N' and (resid 184 through 220 )
96	X-RAY DIFFRACTION	96	chain 'c' and (resid 1 through 37 )
97	X-RAY DIFFRACTION	97	chain 'c' and (resid 38 through 101 )
98	X-RAY DIFFRACTION	98	chain 'c' and (resid 102 through 163 )
99	X-RAY DIFFRACTION	99	chain 'c' and (resid 164 through 218 )
100	X-RAY DIFFRACTION	100	chain 'd' and (resid 2 through 17 )
101	X-RAY DIFFRACTION	101	chain 'd' and (resid 18 through 40 )
102	X-RAY DIFFRACTION	102	chain 'd' and (resid 41 through 51 )
103	X-RAY DIFFRACTION	103	chain 'd' and (resid 52 through 83 )
104	X-RAY DIFFRACTION	104	chain 'd' and (resid 84 through 98 )
105	X-RAY DIFFRACTION	105	chain 'd' and (resid 99 through 134 )
106	X-RAY DIFFRACTION	106	chain 'd' and (resid 135 through 155 )
107	X-RAY DIFFRACTION	107	chain 'd' and (resid 156 through 172 )
108	X-RAY DIFFRACTION	108	chain 'd' and (resid 173 through 203 )
109	X-RAY DIFFRACTION	109	chain 'd' and (resid 204 through 222 )
110	X-RAY DIFFRACTION	110	chain 'V' and (resid 2 through 17 )
111	X-RAY DIFFRACTION	111	chain 'V' and (resid 18 through 64 )
112	X-RAY DIFFRACTION	112	chain 'V' and (resid 65 through 119 )
113	X-RAY DIFFRACTION	113	chain 'V' and (resid 120 through 149 )
114	X-RAY DIFFRACTION	114	chain 'V' and (resid 150 through 165 )
115	X-RAY DIFFRACTION	115	chain 'V' and (resid 166 through 176 )
116	X-RAY DIFFRACTION	116	chain 'V' and (resid 177 through 191 )
117	X-RAY DIFFRACTION	117	chain 'V' and (resid 192 through 203 )
118	X-RAY DIFFRACTION	118	chain 'V' and (resid 204 through 220 )
119	X-RAY DIFFRACTION	119	chain 'W' and (resid 1 through 18 )
120	X-RAY DIFFRACTION	120	chain 'W' and (resid 19 through 80 )
121	X-RAY DIFFRACTION	121	chain 'W' and (resid 81 through 101 )
122	X-RAY DIFFRACTION	122	chain 'W' and (resid 102 through 126 )
123	X-RAY DIFFRACTION	123	chain 'W' and (resid 127 through 163 )
124	X-RAY DIFFRACTION	124	chain 'W' and (resid 164 through 177 )
125	X-RAY DIFFRACTION	125	chain 'W' and (resid 178 through 219 )
126	X-RAY DIFFRACTION	126	chain 'X' and (resid 2 through 134 )
127	X-RAY DIFFRACTION	127	chain 'X' and (resid 135 through 165 )
128	X-RAY DIFFRACTION	128	chain 'X' and (resid 166 through 220 )
129	X-RAY DIFFRACTION	129	chain 'Y' and (resid 1 through 18 )
130	X-RAY DIFFRACTION	130	chain 'Y' and (resid 19 through 37 )
131	X-RAY DIFFRACTION	131	chain 'Y' and (resid 38 through 53 )
132	X-RAY DIFFRACTION	132	chain 'Y' and (resid 54 through 80 )
133	X-RAY DIFFRACTION	133	chain 'Y' and (resid 81 through 101 )
134	X-RAY DIFFRACTION	134	chain 'Y' and (resid 102 through 126 )
135	X-RAY DIFFRACTION	135	chain 'Y' and (resid 127 through 163 )
136	X-RAY DIFFRACTION	136	chain 'Y' and (resid 164 through 192 )
137	X-RAY DIFFRACTION	137	chain 'Y' and (resid 193 through 203 )
138	X-RAY DIFFRACTION	138	chain 'Y' and (resid 204 through 217 )
139	X-RAY DIFFRACTION	139	chain 'Z' and (resid 2 through 138 )
140	X-RAY DIFFRACTION	140	chain 'Z' and (resid 139 through 220 )
141	X-RAY DIFFRACTION	141	chain 'a' and (resid 1 through 126 )
142	X-RAY DIFFRACTION	142	chain 'a' and (resid 127 through 217 )
143	X-RAY DIFFRACTION	143	chain 'b' and (resid 2 through 119 )
144	X-RAY DIFFRACTION	144	chain 'b' and (resid 120 through 220 )
145	X-RAY DIFFRACTION	145	chain 'U' and (resid 1 through 18 )
146	X-RAY DIFFRACTION	146	chain 'U' and (resid 19 through 53 )
147	X-RAY DIFFRACTION	147	chain 'U' and (resid 54 through 80 )
148	X-RAY DIFFRACTION	148	chain 'U' and (resid 81 through 101 )
149	X-RAY DIFFRACTION	149	chain 'U' and (resid 102 through 126 )
150	X-RAY DIFFRACTION	150	chain 'U' and (resid 127 through 163 )
151	X-RAY DIFFRACTION	151	chain 'U' and (resid 164 through 177 )
152	X-RAY DIFFRACTION	152	chain 'U' and (resid 178 through 192 )
153	X-RAY DIFFRACTION	153	chain 'U' and (resid 193 through 203 )
154	X-RAY DIFFRACTION	154	chain 'U' and (resid 204 through 217 )