SH3DOMAIN-CONTAININGKINASE-BINDINGPROTEIN1 / CD2-BINDING PROTEIN 3 / CD2BP3 / CBL-INTERACTING PROTEIN OF 85 KDA / HUMAN SRC FAMILY KINASE- ...CD2-BINDING PROTEIN 3 / CD2BP3 / CBL-INTERACTING PROTEIN OF 85 KDA / HUMAN SRC FAMILY KINASE-BINDING PROTEIN 1 / HSB-1
Mass: 7885.649 Da / Num. of mol.: 1 / Fragment: COILED-COIL DOMAIN, RESIDUES 599-662 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q96B97
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 1.214 Å / Relative weight: 1
Reflection
Resolution: 1.74→45.02 Å / Num. obs: 9541 / % possible obs: 99.6 % / Observed criterion σ(I): 2 / Redundancy: 17.9 % / Rmerge(I) obs: 0.1 / Net I/σ(I): 15.48
Reflection shell
Resolution: 1.74→1.83 Å / Redundancy: 16.88 % / Rmerge(I) obs: 0.92 / Mean I/σ(I) obs: 1.09 / % possible all: 97.6
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Processing
Software
Name
Version
Classification
REFMAC
5.8.0124
refinement
XDS
datareduction
SADABS
datascaling
SHELX
CDE
phasing
Refinement
Method to determine structure: SAD Starting model: NONE Resolution: 1.74→45.02 Å / Cor.coef. Fo:Fc: 0.953 / Cor.coef. Fo:Fc free: 0.918 / SU B: 3.897 / SU ML: 0.116 / Cross valid method: THROUGHOUT / ESU R: 0.13 / ESU R Free: 0.13 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.28327
993
10.4 %
RANDOM
Rwork
0.24397
-
-
-
obs
0.24796
8537
99.86 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK