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- PDB-4tnc: REFINED STRUCTURE OF CHICKEN SKELETAL MUSCLE TROPONIN C IN THE TW... -

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Basic information

Entry
Database: PDB / ID: 4tnc
TitleREFINED STRUCTURE OF CHICKEN SKELETAL MUSCLE TROPONIN C IN THE TWO-CALCIUM STATE AT 2-ANGSTROMS RESOLUTION
ComponentsTROPONIN C
KeywordsCONTRACTILE SYSTEM PROTEIN
Function / homology
Function and homology information


troponin complex / Striated Muscle Contraction / skeletal muscle contraction / calcium ion binding
Similarity search - Function
EF hand / EF-hand domain pair / EF-hand / Recoverin; domain 1 / EF-hand domain pair / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain ...EF hand / EF-hand domain pair / EF-hand / Recoverin; domain 1 / EF-hand domain pair / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
Troponin C, skeletal muscle
Similarity search - Component
Biological speciesGallus gallus (chicken)
MethodX-RAY DIFFRACTION / Resolution: 2 Å
AuthorsSundaralingam, M.
Citation
Journal: J.Biol.Chem. / Year: 1988
Title: Refined structure of chicken skeletal muscle troponin C in the two-calcium state at 2-A resolution.
Authors: Satyshur, K.A. / Rao, S.T. / Pyzalska, D. / Drendel, W. / Greaser, M. / Sundaralingam, M.
#1: Journal: Science / Year: 1985
Title: Molecular Structure of Troponin C from Chicken Skeletal Muscle at 3-Angstrom Resolution
Authors: Sundaralingam, M. / Bergstrom, R. / Strasburg, G. / Rao, S.T. / Roychowdhury, P. / Greaser, M. / Wang, B.C.
#2: Journal: J.Biol.Chem. / Year: 1980
Title: X-Ray Diffraction Studies of Troponin-C Crystals from Rabbit and Chicken Skeletal Muscles
Authors: Strasburg, G.M. / Greaser, M.L. / Sundaralingam, M.
#3: Journal: FEBS Lett. / Year: 1976
Title: The Amino Acid Sequence of Troponin C from Chicken Skeletal Muscle
Authors: Wilkinson, J.M.
History
DepositionSep 28, 1987Processing site: BNL
SupersessionJul 16, 1988ID: 3TNC
Revision 1.0Jul 16, 1988Provider: repository / Type: Initial release
Revision 1.1Mar 25, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 28, 2024Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Other
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_database_status / pdbx_struct_conn_angle / struct_conn / struct_ref_seq_dif / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.process_site / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_ref_seq_dif.details / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: TROPONIN C
hetero molecules


Theoretical massNumber of molelcules
Total (without water)18,3423
Polymers18,2621
Non-polymers802
Water1,22568
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)66.700, 66.700, 60.800
Angle α, β, γ (deg.)90.00, 90.00, 120.00
Int Tables number154
Space group name H-MP3221
Atom site foot note1: SEE REMARK 4.

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Components

#1: Protein TROPONIN C /


Mass: 18262.172 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Gallus gallus (chicken) / References: UniProt: P02588
#2: Chemical ChemComp-CA / CALCIUM ION


Mass: 40.078 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Ca
#3: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 68 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION

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Sample preparation

CrystalDensity Matthews: 2.14 Å3/Da / Density % sol: 42.46 %
Crystal grow
*PLUS
pH: 5.1 / Method: other
Components of the solutions
*PLUS
IDConc.Common nameCrystal-IDSol-IDChemical formula
130 %satammonium sulfate1drop
23 %(w/v)PEG2001drop
350 mMsodium acetate1drop
45 mM1dropMgCl2
51 mM1dropNaN3
643 %satammonium sulfate1reservoir

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Data collection

Reflection
*PLUS
Highest resolution: 2 Å / Lowest resolution: 10 Å / Num. obs: 8861

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Processing

SoftwareName: PROLSQ / Classification: refinement
RefinementResolution: 2→8 Å /
RfactorNum. reflection
obs0.172 8100
Refine analyzeLuzzati coordinate error obs: 0.17 Å
Refinement stepCycle: LAST / Resolution: 2→8 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms1257 0 2 68 1327
Refine LS restraints
Refine-IDTypeDev ideal
X-RAY DIFFRACTIONp_bond_d0.04
X-RAY DIFFRACTIONp_angle_d0.07
Refinement
*PLUS
Highest resolution: 2 Å / Num. reflection obs: 8100 / Rfactor obs: 0.172
Solvent computation
*PLUS
Displacement parameters
*PLUS

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