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Yorodumi- PDB-1jfj: NMR SOLUTION STRUCTURE OF AN EF-HAND CALCIUM BINDING PROTEIN FROM... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1jfj | ||||||
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Title | NMR SOLUTION STRUCTURE OF AN EF-HAND CALCIUM BINDING PROTEIN FROM ENTAMOEBA HISTOLYTICA | ||||||
Components | CALCIUM-BINDING PROTEIN | ||||||
Keywords | METAL BINDING PROTEIN / EF-HAND / HELIX-LOOP-HELIX / CALCIUM-BINDING PROTEIN | ||||||
Function / homology | Function and homology information regulation of actin filament bundle assembly / positive regulation of macropinocytosis / regulation of protein kinase activity / pseudopodium / phagocytic cup / actin monomer binding / phagocytosis / positive regulation of phagocytosis / actin filament binding / calcium ion binding ...regulation of actin filament bundle assembly / positive regulation of macropinocytosis / regulation of protein kinase activity / pseudopodium / phagocytic cup / actin monomer binding / phagocytosis / positive regulation of phagocytosis / actin filament binding / calcium ion binding / endoplasmic reticulum / cytoplasm Similarity search - Function | ||||||
Biological species | Entamoeba histolytica (eukaryote) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Atreya, H.S. / Sahu, S.C. / Bhattacharya, A. / Chary, K.V.R. / Govil, G. | ||||||
Citation | Journal: Biochemistry / Year: 2001 Title: NMR derived solution structure of an EF-hand calcium-binding protein from Entamoeba Histolytica. Authors: Atreya, H.S. / Sahu, S.C. / Bhattacharya, A. / Chary, K.V. / Govil, G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1jfj.cif.gz | 753.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1jfj.ent.gz | 633 KB | Display | PDB format |
PDBx/mmJSON format | 1jfj.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1jfj_validation.pdf.gz | 343.1 KB | Display | wwPDB validaton report |
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Full document | 1jfj_full_validation.pdf.gz | 532.4 KB | Display | |
Data in XML | 1jfj_validation.xml.gz | 60.2 KB | Display | |
Data in CIF | 1jfj_validation.cif.gz | 75.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/jf/1jfj ftp://data.pdbj.org/pub/pdb/validation_reports/jf/1jfj | HTTPS FTP |
-Related structure data
Related structure data | 1jfkC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 14970.852 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Entamoeba histolytica (eukaryote) / Plasmid: PET-3C / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P38505 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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NMR details | Text: The structure was determined using triple-resonance NMR spectroscopy |
-Sample preparation
Details |
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Sample conditions | Ionic strength: 10mM Cacl2 / pH: 6.0 / Pressure: 1 atm / Temperature: 308 K | |||||||||
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian UNITYPLUS / Manufacturer: Varian / Model: UNITYPLUS / Field strength: 600 MHz |
-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 Details: The structure is based on a total of 1265 NOE-derived distance constraints, 200 dihedral angle constraints | ||||||||||||
NMR representative | Selection criteria: fewest violations | ||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 200 / Conformers submitted total number: 20 |