- PDB-4ch1: RRM domain from C. elegans SUP-12 bound to GGTGTGC DNA -
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Basic information
Entry
Database: PDB / ID: 4ch1
Title
RRM domain from C. elegans SUP-12 bound to GGTGTGC DNA
Components
GGTGTGC
PROTEIN SUP-12, ISOFORM B
Keywords
TRANSCRIPTION/DNA / TRANSCRIPTION-DNA COMPLEX / MUSCLE / DEVELOPMENT
Function / homology
Function and homology information
alternative mRNA splicing, via spliceosome / pre-mRNA binding / regulation of locomotion / regulation of alternative mRNA splicing, via spliceosome / pre-mRNA intronic binding / regulation of actin cytoskeleton organization / nuclear speck / ribonucleoprotein complex Similarity search - Function
Mass: 10831.196 Da / Num. of mol.: 1 / Fragment: RRM DOMAIN, RESIDUES 28-121 Source method: isolated from a genetically manipulated source Source: (gene. exp.) CAENORHABDITIS ELEGANS (invertebrata) / Plasmid: PET-HIS1A / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): PLYSY / References: UniProt: H2L051
#2: DNA chain
GGTGTGC
Mass: 2169.433 Da / Num. of mol.: 1 / Fragment: SUP-12 BINDING MOTIF / Source method: obtained synthetically Details: THE HEPTAMER SEQUENCE IS DERIVED FROM THE INTRON BETWEEN EXONS 4 AND 5B FROM THE C. ELEGANS EGL-15 GENE. Source: (synth.) CAENORHABDITIS ELEGANS (invertebrata)
Sequence details
THE SAMPLE CONTAINS AN ADDITIONAL GLY-ALA-MET- AT THE N- TERMINUS FOLLOWING CLEAVAGE BY TEV PROTEASE
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
15N-NOESY
2
2
1
13C-NOESY
3
3
1
DOUBLE- FILTERED 1H
4
4
1
1H- NOESY
5
5
1
1H-TOCSY
6
6
1
1H-NOESY
NMR details
Text: THE STRUCTURE WAS DETERMINED BY USING MULTI- DIMENSIONAL NMR SPECTRSOCOPY WITH 15N-, 13C,15N-, OR 2H- LABELED SUP-12 IN COMPLEX WITH A NATURAL ABUNDANCE GGTGTGC DNA LIGAND, OR A LIGAND WITH ...Text: THE STRUCTURE WAS DETERMINED BY USING MULTI- DIMENSIONAL NMR SPECTRSOCOPY WITH 15N-, 13C,15N-, OR 2H- LABELED SUP-12 IN COMPLEX WITH A NATURAL ABUNDANCE GGTGTGC DNA LIGAND, OR A LIGAND WITH SITE-SPECIFIC 13C,15N- LABELING.
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