+Open data
-Basic information
Entry | Database: PDB / ID: 3ez9 | ||||||
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Title | Partition Protein | ||||||
Components | ParA | ||||||
Keywords | BIOSYNTHETIC PROTEIN / dna binding / winged-hth / partition | ||||||
Function / homology | Multidrug-efflux Transporter Regulator; Chain: A; Domain 2 - #30 / Multidrug-efflux Transporter Regulator; Chain: A; Domain 2 / P-loop containing nucleotide triphosphate hydrolases / Rossmann fold / Orthogonal Bundle / 3-Layer(aba) Sandwich / Mainly Alpha / Alpha Beta / : Function and homology information | ||||||
Biological species | Salmonella enterica subsp. enterica serovar Newport str. SL317 (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2.8 Å | ||||||
Authors | Schumacher, M.A. | ||||||
Citation | Journal: Embo J. / Year: 2009 Title: Structural basis for ADP-mediated transcriptional regulation by P1 and P7 ParA. Authors: Dunham, T.D. / Xu, W. / Funnell, B.E. / Schumacher, M.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3ez9.cif.gz | 152.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3ez9.ent.gz | 121.3 KB | Display | PDB format |
PDBx/mmJSON format | 3ez9.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ez/3ez9 ftp://data.pdbj.org/pub/pdb/validation_reports/ez/3ez9 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Details | dimer |
-Components
#1: Protein | Mass: 45534.625 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Salmonella enterica subsp. enterica serovar Newport str. SL317 (bacteria) Gene: SNSL317_A0932 / References: UniProt: B4ABW6 #2: Chemical | ChemComp-MG / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.18 Å3/Da / Density % sol: 61.28 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: Peg 3000, ethylene glycol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 8.2.1 / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Jan 2, 2007 / Details: mirrors |
Radiation | Monochromator: mirrors / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.78→100.5 Å / Num. obs: 33374 / % possible obs: 96 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3 % / Biso Wilson estimate: 52.7 Å2 / Rsym value: 0.048 / Net I/σ(I): 10.1 |
-Processing
Software | Name: CNS / Version: 1.2 / Classification: refinement | |||||||||||||||||||||||||||
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Refinement | Resolution: 2.8→66.85 Å / Rfactor Rfree error: 0.007 / Occupancy max: 1 / Occupancy min: 0.5 / Data cutoff high absF: 2254383 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Details: BULK SOLVENT MODEL USED
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 58.177 Å2 / ksol: 0.3 e/Å3 | |||||||||||||||||||||||||||
Displacement parameters | Biso max: 184.8 Å2 / Biso mean: 87.502 Å2 / Biso min: 20 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.8→66.85 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.8→2.98 Å / Rfactor Rfree error: 0.023 / Total num. of bins used: 6
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Xplor file |
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