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Open data
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Basic information
Entry | Database: PDB / ID: 6w3j | |||||||||
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Title | Crystal structure of the FAM46C/Plk4/Cep192 complex | |||||||||
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![]() | TRANSFERASE / RNA polymerase | |||||||||
Function / homology | ![]() centrosome-templated microtubule nucleation / de novo centriole assembly involved in multi-ciliated epithelial cell differentiation / procentriole / deuterosome / procentriole replication complex / positive regulation of centriole replication / trophoblast giant cell differentiation / centriole replication / polo kinase / XY body ...centrosome-templated microtubule nucleation / de novo centriole assembly involved in multi-ciliated epithelial cell differentiation / procentriole / deuterosome / procentriole replication complex / positive regulation of centriole replication / trophoblast giant cell differentiation / centriole replication / polo kinase / XY body / poly(A) RNA polymerase activity / polynucleotide adenylyltransferase / mRNA stabilization / protein localization to centrosome / pericentriolar material / cleavage furrow / negative regulation of cell differentiation / mitotic spindle assembly / cilium assembly / phosphatase binding / Loss of Nlp from mitotic centrosomes / Loss of proteins required for interphase microtubule organization from the centrosome / Recruitment of mitotic centrosome proteins and complexes / Recruitment of NuMA to mitotic centrosomes / Anchoring of the basal body to the plasma membrane / centriole / AURKA Activation by TPX2 / response to bacterium / Regulation of PLK1 Activity at G2/M Transition / in utero embryonic development / protein phosphorylation / protein serine kinase activity / protein serine/threonine kinase activity / centrosome / nucleolus / protein kinase binding / RNA binding / nucleoplasm / ATP binding / identical protein binding / nucleus / cytoplasm / cytosol Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Chen, H. / Lu, D.F. / Shang, G.J. / Zhang, X.W. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural and Functional Analyses of the FAM46C/Plk4 Complex. Authors: Chen, H. / Lu, D. / Shang, G. / Gao, G. / Zhang, X. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 247.4 KB | Display | ![]() |
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PDB format | ![]() | 199.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 441 KB | Display | ![]() |
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Full document | ![]() | 448.5 KB | Display | |
Data in XML | ![]() | 22 KB | Display | |
Data in CIF | ![]() | 29 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6w36C ![]() 6w38C ![]() 6w3iC ![]() 4n7zS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 40035.801 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: Q5VWP2, polynucleotide adenylyltransferase |
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#2: Protein | Mass: 25869.609 Da / Num. of mol.: 1 / Fragment: UNP residues 585-807 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
#3: Protein/peptide | Mass: 2632.828 Da / Num. of mol.: 1 / Fragment: UNP residues 217-238 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.34 Å3/Da / Density % sol: 71.67 % |
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Crystal grow | Temperature: 293 K / Method: small tubes / pH: 10 / Details: 0.2 M Tris, pH 10 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Feb 15, 2017 |
Radiation | Monochromator: double crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9794 Å / Relative weight: 1 |
Reflection | Resolution: 4.38→50 Å / Num. obs: 7771 / % possible obs: 99.1 % / Redundancy: 18.7 % / Rpim(I) all: 0.023 / Net I/σ(I): 26.9 |
Reflection shell | Resolution: 4.38→4.48 Å / Num. unique obs: 367 / CC1/2: 0.891 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 4N7Z Resolution: 4.385→37.166 Å / SU ML: 0.6 / Cross valid method: FREE R-VALUE / σ(F): 1.49 / Phase error: 31.51
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 4.385→37.166 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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