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Yorodumi- PDB-2pcv: Solution Structure of an rRNA Substrate Bound to the Pseudouridyl... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2pcv | ||||||
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Title | Solution Structure of an rRNA Substrate Bound to the Pseudouridylation Pocket of a Box H/ACA snoRNA | ||||||
Components | U65 Box H/ACA snoRNA | ||||||
Keywords | RNA / box H/ACA snoRNA / pseudouridylation / rRNA | ||||||
Function / homology | RNA / RNA (> 10) Function and homology information | ||||||
Method | SOLUTION NMR / torsion angle dynamics,simulated annealing | ||||||
Authors | Jin, H. / Loria, J.P. / Moore, P.B. | ||||||
Citation | Journal: Mol.Cell / Year: 2007 Title: Solution structure of an rRNA substrate bound to the pseudouridylation pocket of a box H/ACA snoRNA. Authors: Jin, H. / Loria, J.P. / Moore, P.B. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2pcv.cif.gz | 220.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2pcv.ent.gz | 185.7 KB | Display | PDB format |
PDBx/mmJSON format | 2pcv.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2pcv_validation.pdf.gz | 319.7 KB | Display | wwPDB validaton report |
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Full document | 2pcv_full_validation.pdf.gz | 370.8 KB | Display | |
Data in XML | 2pcv_validation.xml.gz | 6 KB | Display | |
Data in CIF | 2pcv_validation.cif.gz | 10.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/pc/2pcv ftp://data.pdbj.org/pub/pdb/validation_reports/pc/2pcv | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: RNA chain | Mass: 11233.758 Da / Num. of mol.: 1 Fragment: RNA hairpin containing the pseudouridylation pocket Source method: obtained synthetically Details: Synthesized by in vitro transcription using T7 RNA polymerase from linearized plasmid DNA. This sequence naturally occurs in humans. |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: SEE PAPER FOR MORE DETAILS |
-Sample preparation
Details |
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Sample conditions |
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-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | ||||||||||||||||||||
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Radiation wavelength | Relative weight: 1 | ||||||||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics,simulated annealing / Software ordinal: 1 / Details: described in the primary citation | ||||||||
NMR representative | Selection criteria: closest to the average | ||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 50 / Conformers submitted total number: 10 |