[English] 日本語
![](img/lk-miru.gif)
- PDB-2pcw: Solution Structure of an rRNA Substrate Bound to the Pseudouridyl... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 2pcw | ||||||
---|---|---|---|---|---|---|---|
Title | Solution Structure of an rRNA Substrate Bound to the Pseudouridylation Pocket of a Box H/ACA snoRNA | ||||||
![]() |
| ||||||
![]() | RNA / box H/ACA snoRNA / pseudouridylation pocket / rRNA | ||||||
Function / homology | RNA / RNA (> 10)![]() | ||||||
Method | SOLUTION NMR / torsion angle dynamics molecular dynamics | ||||||
![]() | Jin, H. / Loria, J.P. / Moore, P.B. | ||||||
![]() | ![]() Title: Solution structure of an rRNA substrate bound to the pseudouridylation pocket of a box H/ACA snoRNA. Authors: Jin, H. / Loria, J.P. / Moore, P.B. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 515.1 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 436.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 336.5 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 529.8 KB | Display | |
Data in XML | ![]() | 12.7 KB | Display | |
Data in CIF | ![]() | 24.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
-
Links
-
Assembly
Deposited unit | ![]()
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-
Components
#1: RNA chain | Mass: 11233.758 Da / Num. of mol.: 1 Fragment: RNA Hairpin containing the 5' pseudouridylation pocket Source method: obtained synthetically Details: Synthesized by in-vitro transcription using T7 RNA polymerase from linearzed plsmid DNA. This sequence occurs naturally in humans. |
---|---|
#2: RNA chain | Mass: 4495.691 Da / Num. of mol.: 1 Fragment: rRNA substrate of the 5' pseudouridylation pocket of U65 Box H/ACA snoRNA Source method: obtained synthetically Details: Chemically synthesized. This sequence occurs naturally in humans. |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
| ||||||||||||||||||||||||||||||||||||||||
NMR details | Text: See paper for more details |
-
Sample preparation
Details |
| |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sample conditions |
|
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Radiation wavelength | Relative weight: 1 | ||||||||||||||||||||
NMR spectrometer |
|
-
Processing
NMR software |
| ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: torsion angle dynamics molecular dynamics / Software ordinal: 1 / Details: Described in the primary citation | ||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 50 / Conformers submitted total number: 17 |