+Open data
-Basic information
Entry | Database: PDB / ID: 2kn4 | ||||||
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Title | The structure of the RRM domain of SC35 | ||||||
Components | Immunoglobulin G-binding protein G,Serine/arginine-rich splicing factor 2 | ||||||
Keywords | RNA BINDING PROTEIN / RRM domain / Cell wall / IgG-binding protein / Peptidoglycan-anchor / Secreted / mRNA processing / mRNA splicing / Nucleus / Phosphoprotein / RNA-binding | ||||||
Function / homology | Function and homology information interchromatin granule / perichromatin fibrils / mRNA 3'-end processing / pre-mRNA binding / Transport of Mature mRNA derived from an Intron-Containing Transcript / IgG binding / RNA Polymerase II Transcription Termination / regulation of alternative mRNA splicing, via spliceosome / mRNA Splicing - Minor Pathway / Processing of Capped Intron-Containing Pre-mRNA ...interchromatin granule / perichromatin fibrils / mRNA 3'-end processing / pre-mRNA binding / Transport of Mature mRNA derived from an Intron-Containing Transcript / IgG binding / RNA Polymerase II Transcription Termination / regulation of alternative mRNA splicing, via spliceosome / mRNA Splicing - Minor Pathway / Processing of Capped Intron-Containing Pre-mRNA / response to vitamin E / mRNA Splicing - Major Pathway / RNA splicing / protein kinase C binding / spliceosomal complex / mRNA splicing, via spliceosome / mRNA processing / transcription corepressor activity / nuclear speck / RNA binding / extracellular region / nucleoplasm / nucleus / cytosol Similarity search - Function | ||||||
Biological species | Streptococcus sp. group G (bacteria) Homo sapiens (human) | ||||||
Method | SOLUTION NMR / simulated annealing, molecular dynamics, torsion angle dynamics | ||||||
Model details | lowest energy, model 1 | ||||||
Authors | Clayton, J.C. / Goult, B.T. / Lian, L.-Y. | ||||||
Citation | Journal: Nucleic Acids Res. / Year: 2011 Title: The structure and selectivity of the SR protein SRSF2 RRM domain with RNA Authors: Phelan, M.M. / Goult, B.T. / Clayton, J.C. / Hautbergue, G.M. / Wilson, S.A. / Lian, L.-Y. | ||||||
History |
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Remark 700 | SHEET DETERMINATION METHOD: AUTHOR DETERMINED. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2kn4.cif.gz | 961 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2kn4.ent.gz | 809.2 KB | Display | PDB format |
PDBx/mmJSON format | 2kn4.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2kn4_validation.pdf.gz | 347.8 KB | Display | wwPDB validaton report |
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Full document | 2kn4_full_validation.pdf.gz | 494.3 KB | Display | |
Data in XML | 2kn4_validation.xml.gz | 66.6 KB | Display | |
Data in CIF | 2kn4_validation.cif.gz | 87.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kn/2kn4 ftp://data.pdbj.org/pub/pdb/validation_reports/kn/2kn4 | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Antibody | Mass: 18133.104 Da / Num. of mol.: 1 Fragment: UNP residues 304-357 from P19909, UNP residues 9-101 from Q01130 Source method: isolated from a genetically manipulated source Details: Chimera of Immunoglobulin G-binding protein G, linker, Splicing factor; arginine/serine-rich 2 Source: (gene. exp.) Streptococcus sp. group G (bacteria), (gene. exp.) Homo sapiens (human) Gene: spg, SRSF2, SFRS2 / Production host: Escherichia coli (E. coli) / References: UniProt: P19909, UniProt: Q01130 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 100 / pH: 6.8 / Pressure: ambient / Temperature: 305 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing, molecular dynamics, torsion angle dynamics Software ordinal: 1 Details: FINAL STRUCTURES REFINED IN EXPLICIT WATER BATH AS IMPLEMENTED IN ARIA 1.2/CNS 1.1. 20 LOWEST ENERGY STRUCTURES SELECTED FROM WATER REFINEMENT USING CNS. INITIAL STRUCTURES GENERATED WITH CYANA | ||||||||||||||||||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 / Representative conformer: 1 |