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Open data
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Basic information
Entry | Database: PDB / ID: 6kwj | ||||||
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Title | A Crystal Structure of OspA mutant | ||||||
![]() | Outer surface protein A | ||||||
![]() | LIPID BINDING PROTEIN / Outer surface protein A / OspA | ||||||
Function / homology | Outer surface lipoprotein, Borrelia / Outer surface lipoprotein domain superfamily / Borrelia lipoprotein / cell outer membrane / Prokaryotic membrane lipoprotein lipid attachment site profile. / cell surface / membrane / Outer surface protein A![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kiya, M. / Makabe, K. | ||||||
![]() | ![]() Title: beta-Strand-mediated Domain-swapping in the Absence of Hydrophobic Core Repacking. Authors: Kiya, M. / Shiga, S. / Ding, P. / Koide, S. / Makabe, K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 68.5 KB | Display | ![]() |
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PDB format | ![]() | 44.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6kwuC ![]() 6kwvC ![]() 6ljyC ![]() 7fddC ![]() 7fjrC ![]() 2g8cS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 25416.320 Da / Num. of mol.: 1 Mutation: E37S,E45S,K46S,K48A,K60A,K64S,K83A,E104S,K107S,K117G,F126G,N127G,K239S,E240S,K254S Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680 / Gene: ospA, BB_A15 / Production host: ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.01 Å3/Da / Density % sol: 59.07 % |
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Crystal grow | Temperature: 282 K / Method: vapor diffusion, hanging drop / pH: 7 / Details: 26%PEG 400, 0.1M Imidozole, 16.1 mg/mL |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 S 2M / Detector: PIXEL / Date: Jun 8, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.94→19.9 Å / Num. obs: 22185 / % possible obs: 99.4 % / Redundancy: 3.2 % / Biso Wilson estimate: 26.6 Å2 / Rmerge(I) obs: 0.049 / Net I/σ(I): 28.2 |
Reflection shell | Resolution: 1.94→1.99 Å / Redundancy: 3 % / Rmerge(I) obs: 0.44 / Mean I/σ(I) obs: 11.6 / Num. unique obs: 1123 / % possible all: 99.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2G8C Resolution: 1.94→19.9 Å / SU ML: 0.259 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 26.4942 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 31.09 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.94→19.9 Å
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Refine LS restraints |
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LS refinement shell |
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