+Open data
-Basic information
Entry | Database: PDB / ID: 2eh7 | ||||||
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Title | Crystal structure of humanized KR127 FAB | ||||||
Components |
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Keywords | IMMUNE SYSTEM / HEPATITIS B VIRUS / HUMANIZED ANTIBODY / MONOCLONAL ANTIBODY / NEUTRALIZATION / PRES1 | ||||||
Function / homology | Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta Function and homology information | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.5 Å | ||||||
Authors | Chi, S.-W. / Kim, S.-J. / Maeng, C.-Y. / Hong, H.J. / Ryu, S.-E. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.Usa / Year: 2007 Title: Broadly neutralizing anti-hepatitis B virus antibody reveals a complementarity determining region H3 lid-opening mechanism Authors: Chi, S.-W. / Maeng, C.-Y. / Kim, S.J. / Oh, M.S. / Ryu, C.J. / Kim, S.-J. / Han, K.-H. / Hong, H.J. / Ryu, S.-E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2eh7.cif.gz | 94.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2eh7.ent.gz | 71.4 KB | Display | PDB format |
PDBx/mmJSON format | 2eh7.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2eh7_validation.pdf.gz | 431.9 KB | Display | wwPDB validaton report |
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Full document | 2eh7_full_validation.pdf.gz | 443 KB | Display | |
Data in XML | 2eh7_validation.xml.gz | 17.8 KB | Display | |
Data in CIF | 2eh7_validation.cif.gz | 23.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/eh/2eh7 ftp://data.pdbj.org/pub/pdb/validation_reports/eh/2eh7 | HTTPS FTP |
-Related structure data
Related structure data | 2eh8C 1aj7S C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Antibody | Mass: 23891.732 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: HUMANIZED KR127 / Plasmid: PCMV / Cell (production host): DHFR-DEFICIENT CHO CELL / Cell line (production host): DG44 / Production host: Homo sapiens (human) |
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#2: Antibody | Mass: 23086.756 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: HUMANIZED KR127 / Plasmid: PCMV / Cell (production host): DHFR-DEFICIENT CHO CELL / Cell line (production host): DG44 / Production host: Homo sapiens (human) |
Sequence details | A SEQUENCE DATABASE REFERENCE FOR THIS PROTEIN DOES NOT CURRENTLY EXIST. THE SEQUENCES ARE SHOWN IN ...A SEQUENCE DATABASE REFERENCE FOR THIS PROTEIN DOES NOT CURRENTLY EXIST. THE SEQUENCES ARE SHOWN IN THE PAPER(FIGURE 1): HONG, H.J. ET AL., VIROLOGY 318, 134-141 (2004) |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.52 Å3/Da / Density % sol: 51.23 % |
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Crystal grow | Temperature: 288 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 17% PEG 4000, 10mM Hepes-NaOH, 0.2M ammonium sulfate, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU300 / Wavelength: 1.5418 / Wavelength: 1.5418 Å |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Jan 9, 2001 |
Radiation | Monochromator: CONFOCAL MIRROR / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→31.31 Å / Num. all: 16404 / Num. obs: 15758 / % possible obs: 96.3 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.078 / Net I/σ(I): 5.9 |
Reflection shell | Resolution: 2.5→2.66 Å / % possible all: 97.5 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1AJ7 Resolution: 2.5→31.31 Å / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0
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Refinement step | Cycle: LAST / Resolution: 2.5→31.31 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.5→2.66 Å / % reflection obs: 97.5 % |