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Yorodumi- PDB-2cha: THE STRUCTURE OF CRYSTALLINE ALPHA-CHYMOTRYPSIN, $V.THE ATOMIC ST... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2cha | |||||||||
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Title | THE STRUCTURE OF CRYSTALLINE ALPHA-CHYMOTRYPSIN, $V.THE ATOMIC STRUCTURE OF TOSYL-ALPHA-CHYMOTRYPSIN AT 2 ANGSTROMS RESOLUTION | |||||||||
Components | (ALPHA-CHYMOTRYPSIN AChymotrypsin) x 3 | |||||||||
Keywords | HYDROLASE (SERINE PROTEINASE) | |||||||||
Function / homology | Function and homology information chymotrypsin / serpin family protein binding / serine protease inhibitor complex / digestion / serine-type endopeptidase activity / proteolysis / extracellular region Similarity search - Function | |||||||||
Biological species | Bos taurus (cattle) | |||||||||
Method | X-RAY DIFFRACTION / Resolution: 2 Å | |||||||||
Authors | Birktoft, J.J. / Blow, D.M. | |||||||||
Citation | Journal: J.Mol.Biol. / Year: 1972 Title: Structure of crystalline -chymotrypsin. V. The atomic structure of tosyl- -chymotrypsin at 2 A resolution. Authors: Birktoft, J.J. / Blow, D.M. #1: Journal: Nature / Year: 1967 Title: Three-Dimensional Structure of Tosyl-Alpha-Chymotrypsin Authors: Matthews, B.W. / Sigler, P.B. / Henderson, R. / Blow, D.M. #2: Journal: J.Mol.Biol. / Year: 1968 Title: Structure of Crystalline Alpha-Chymotrypsin, II.A Preliminary Report Including a Hypothesis for the Activation Mechanism Authors: Sigler, P.B. / Blow, D.M. / Matthews, B.W. / Henderson, R. #3: Journal: J.Mol.Biol. / Year: 1969 Title: Structure of Crystalline Alpha-Chymotrypsin, III.Crystallographic Studies of Substrates and Inhibitors Bound to the Active Site of Alpha-Chymotrypsin Authors: Steitz, T.A. / Henderson, R. / Blow, D.M. #4: Journal: J.Mol.Biol. / Year: 1970 Title: Structure of Crystalline Alpha-Chymotrypsin, Iv.The Structure of Indoleacryloyl-Alpha-Chymotrypsin and its Relevance to the Hydrolytic Mechanism of the Enzyme Authors: Henderson, R. #5: Journal: Acc.Chem.Res. / Year: 1976 Title: Structure and Mechanism of Chymotrypsin Authors: Blow, D.M. #6: Journal: Ann.N.Y.Acad.Sci. / Year: 1974 Title: The Active Centers of Serine Proteinases Authors: Hartley, B.S. #7: Journal: J.Mol.Biol. / Year: 1973 Title: Comparison of the Crystal Structures of Chymotrypsinogen-A and Alpha-Chymotrypsin Authors: Wright, H.T. #8: Journal: J.Mol.Biol. / Year: 1972 Title: Structure of Crystalline Methyl-Chymotrypsin Authors: Wright, C.S. / Hess, G.P. / Blow, D.M. #9: Journal: Cold Spring Harbor Symp.Quant.Biol. / Year: 1972 Title: Alpha-Chymotrypsin,What Can We Learn About Catalysis from X-Ray Diffraction (Query). Authors: Henderson, R. / Wright, C.S. / Hess, G.P. / Blow, D.M. #10: Journal: The Enzymes,Third Edition / Year: 1971 Title: The Structure of Chymotrypsin Authors: Blow, D.M. #11: Journal: The Enzymes,Third Edition / Year: 1971 Title: Chymotrypsin-Chemical Properties and Catalysis Authors: Hess, G.P. #12: Journal: Philos.Trans.R.Soc.London,Ser.B / Year: 1970 Title: The Structure of Alpha-Chymotrypsin Authors: Birktoft, J.J. / Blow, D.M. / Henderson, R. / Steitz, T.A. #13: Journal: Biochem.J. / Year: 1969 Title: The Study of Alpha-Chymotrypsin by X-Ray Diffraction Authors: Blow, D.M. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2cha.cif.gz | 91.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2cha.ent.gz | 67.7 KB | Display | PDB format |
PDBx/mmJSON format | 2cha.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ch/2cha ftp://data.pdbj.org/pub/pdb/validation_reports/ch/2cha | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: THE SIDE-CHAIN HYDROXYL GROUP OF SERINE 195 IS TOSYLATED IN THIS STRUCTURE. COORDINATES FOR THE P-TOLUENE-SULFONATE GROUP ARE GIVEN BELOW IN THE *HETATM* RECORDS FOR THE GROUP *TOS*. | ||||||||
Noncrystallographic symmetry (NCS) | NCS oper: (Code: given / Matrix: (0.917752, 0.397154), |
-Components
#1: Protein/peptide | Mass: 1253.511 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Bos taurus (cattle) / References: UniProt: P00766, chymotrypsin #2: Protein | Mass: 13934.556 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Bos taurus (cattle) / References: UniProt: P00766, chymotrypsin #3: Protein | Mass: 10074.495 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Bos taurus (cattle) / References: UniProt: P00766, chymotrypsin #4: Chemical | #5: Water | ChemComp-HOH / | Compound details | 2CHA THE ALPHA CHYMOTRYPSIN MOLECULE IS COMPRISED OF THREE 2CHA POLYPEPTIDE CHAINS WHICH ARE ...2CHA THE ALPHA CHYMOTRYPS | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 4.23 Å3/Da / Density % sol: 70.89 % |
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Crystal grow | *PLUS Method: unknown |
-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
-Processing
Refinement | Highest resolution: 2 Å | ||||||||||||
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Refinement step | Cycle: LAST / Highest resolution: 2 Å
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