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- PDB-2c52: Structural diversity in CBP p160 complexes -

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Basic information

Entry
Database: PDB / ID: 2c52
TitleStructural diversity in CBP p160 complexes
Components
  • CREB-BINDING PROTEIN
  • NUCLEAR RECEPTOR COACTIVATOR 1
KeywordsTRANSFERASE / ACTIVATOR / BROMODOMAIN / METAL-BINDING / METHYLATION / NUCLEAR PROTEIN / TRANSCRIPTION / TRANSCRIPTION REGULATION / ZINC / ZINC-FINGER / ACYLTRANSFERASE / ALTERNATIVE SPLICING / CHROMOSOMAL TRANSLOCATION / POLYMORPHISM / PROTO-ONCOGENE / UBL CONJUGATION
Function / homology
Function and homology information


Activation of the TFAP2 (AP-2) family of transcription factors / Regulation of FOXO transcriptional activity by acetylation / TRAF6 mediated IRF7 activation / Nuclear events mediated by NFE2L2 / Attenuation phase / Regulation of gene expression by Hypoxia-inducible Factor / LRR FLII-interacting protein 1 (LRRFIP1) activates type I IFN production / RUNX1 regulates transcription of genes involved in differentiation of myeloid cells / cAMP response element binding protein binding / Formation of the beta-catenin:TCF transactivating complex ...Activation of the TFAP2 (AP-2) family of transcription factors / Regulation of FOXO transcriptional activity by acetylation / TRAF6 mediated IRF7 activation / Nuclear events mediated by NFE2L2 / Attenuation phase / Regulation of gene expression by Hypoxia-inducible Factor / LRR FLII-interacting protein 1 (LRRFIP1) activates type I IFN production / RUNX1 regulates transcription of genes involved in differentiation of myeloid cells / cAMP response element binding protein binding / Formation of the beta-catenin:TCF transactivating complex / NOTCH1 Intracellular Domain Regulates Transcription / RUNX3 regulates NOTCH signaling / Notch-HLH transcription pathway / positive regulation of cell adhesion molecule production / germ-line stem cell population maintenance / Regulation of lipid metabolism by PPARalpha / negative regulation of viral process / Cytoprotection by HMOX1 / CD209 (DC-SIGN) signaling / Estrogen-dependent gene expression / outer kinetochore / negative regulation of interferon-beta production / histone H3K27 acetyltransferase activity / histone H3K18 acetyltransferase activity / N-terminal peptidyl-lysine acetylation / MRF binding / labyrinthine layer morphogenesis / regulation of thyroid hormone mediated signaling pathway / positive regulation of transcription from RNA polymerase II promoter by galactose / peroxisome proliferator activated receptor binding / positive regulation of female receptivity / face morphogenesis / negative regulation of transcription by RNA polymerase I / positive regulation of dendritic spine development / peptide-lysine-N-acetyltransferase activity / hypothalamus development / male mating behavior / cellular response to hepatocyte growth factor stimulus / NR1H2 & NR1H3 regulate gene expression to control bile acid homeostasis / positive regulation of transforming growth factor beta receptor signaling pathway / SMAD binding / behavioral response to cocaine / non-canonical NF-kappaB signal transduction / acetyltransferase activity / cellular response to nutrient levels / estrous cycle / TFIIB-class transcription factor binding / cellular response to Thyroglobulin triiodothyronine / histone acetyltransferase complex / Synthesis of bile acids and bile salts / positive regulation of G1/S transition of mitotic cell cycle / long-term memory / Endogenous sterols / Synthesis of bile acids and bile salts via 27-hydroxycholesterol / Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol / nuclear retinoid X receptor binding / NR1H3 & NR1H2 regulate gene expression linked to cholesterol transport and efflux / response to retinoic acid / histone acetyltransferase activity / Recycling of bile acids and salts / regulation of cellular response to insulin stimulus / histone acetyltransferase / cellular response to hormone stimulus / Transferases; Acyltransferases; Transferring groups other than aminoacyl groups / positive regulation of adipose tissue development / peroxisome proliferator activated receptor signaling pathway / positive regulation of neuron differentiation / RORA activates gene expression / lactation / Regulation of lipid metabolism by PPARalpha / cerebellum development / BMAL1:CLOCK,NPAS2 activates circadian gene expression / SUMOylation of transcription cofactors / nuclear receptor coactivator activity / Activation of gene expression by SREBF (SREBP) / response to progesterone / nuclear estrogen receptor binding / nuclear receptor binding / hippocampus development / RNA polymerase II transcription regulatory region sequence-specific DNA binding / protein modification process / protein destabilization / Heme signaling / mRNA transcription by RNA polymerase II / Transcriptional activation of mitochondrial biogenesis / transcription coactivator binding / PML body / PPARA activates gene expression / Cytoprotection by HMOX1 / cerebral cortex development / chromatin DNA binding / cellular response to virus / Transcriptional regulation of white adipocyte differentiation / positive regulation of non-canonical NF-kappaB signal transduction / positive regulation of protein localization to nucleus / RNA polymerase II transcription regulator complex / male gonad development / transcription corepressor activity / cellular response to UV / rhythmic process
Similarity search - Function
Helix Hairpins - #410 / Creb-binding Protein; Chain: A / Nuclear receptor coactivator, CREB-bp-like, interlocking domain / Nuclear receptor coactivator, CREB-bp-like, interlocking / Nuclear receptor coactivator, CREB-bp-like, interlocking domain superfamily / Creb binding / Zinc finger, TAZ-type / TAZ domain superfamily / TAZ zinc finger / Zinc finger TAZ-type profile. ...Helix Hairpins - #410 / Creb-binding Protein; Chain: A / Nuclear receptor coactivator, CREB-bp-like, interlocking domain / Nuclear receptor coactivator, CREB-bp-like, interlocking / Nuclear receptor coactivator, CREB-bp-like, interlocking domain superfamily / Creb binding / Zinc finger, TAZ-type / TAZ domain superfamily / TAZ zinc finger / Zinc finger TAZ-type profile. / TAZ zinc finger, present in p300 and CBP / Coactivator CBP, KIX domain / CREB-binding protein/p300, atypical RING domain / CBP/p300-type histone acetyltransferase domain / CBP/p300, atypical RING domain superfamily / KIX domain / CREB-binding protein/p300, atypical RING domain / KIX domain profile. / CBP/p300-type histone acetyltransferase (HAT) domain profile. / Histone acetyltransferase Rtt109/CBP / Histone acetylation protein / Histone acetylation protein / Coactivator CBP, KIX domain superfamily / Nuclear receptor coactivator 1 / Zinc finger ZZ-type signature. / Zinc-binding domain, present in Dystrophin, CREB-binding protein. / Zinc finger, ZZ type / Zinc finger, ZZ-type / Zinc finger, ZZ-type superfamily / Zinc finger ZZ-type profile. / Helix Hairpins / Nuclear receptor coactivator, DUF1518 / Nuclear receptor coactivator, Ncoa-type, interlocking / Nuclear receptor coactivator, Ncoa-type, interlocking domain superfamily / Nuclear receptor coactivator, DUF1518 / Nuclear receptor coactivator / DUF1518 / Nuclear receptor coactivator, receptor-binding domain / Nuclear receptor coactivator / Steroid receptor coactivator / Unstructured region on nuclear receptor coactivator protein / PAS domain / Nuclear receptor coactivator, interlocking / Helix-loop-helix DNA-binding domain superfamily / helix loop helix domain / Myc-type, basic helix-loop-helix (bHLH) domain / Myc-type, basic helix-loop-helix (bHLH) domain profile. / PAS fold / PAS fold / PAS domain / PAS repeat profile. / PAS domain / PAS domain superfamily / Helix non-globular / Special / Bromodomain, conserved site / Bromodomain signature. / Bromodomain / Bromodomain profile. / bromo domain / Bromodomain / Bromodomain-like superfamily / Zinc finger, RING/FYVE/PHD-type / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
Histone lysine acetyltransferase CREBBP / Nuclear receptor coactivator 1
Similarity search - Component
Biological speciesMUS MUSCULUS (house mouse)
HOMO SAPIENS (human)
MethodSOLUTION NMR / CANDID
AuthorsWaters, L.C. / Yue, B. / Veverka, V. / Renshaw, P.S. / Bramham, J. / Matsuda, S. / Frenkiel, T. / Kelly, G. / Muskett, F.W. / Carr, M.D. / Heery, D.M.
CitationJournal: J. Biol. Chem. / Year: 2006
Title: Structural diversity in p160/CREB-binding protein coactivator complexes.
Authors: Waters, L. / Yue, B. / Veverka, V. / Renshaw, P. / Bramham, J. / Matsuda, S. / Frenkiel, T. / Kelly, G. / Muskett, F. / Carr, M. / Heery, D.M.
History
DepositionOct 25, 2005Deposition site: PDBE / Processing site: PDBE
Revision 1.0Mar 15, 2006Provider: repository / Type: Initial release
Revision 1.1May 8, 2011Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3May 2, 2018Group: Data collection / Database references
Category: citation / citation_author / pdbx_nmr_spectrometer
Item: _citation.journal_abbrev / _citation.page_last ..._citation.journal_abbrev / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.title / _citation_author.name / _pdbx_nmr_spectrometer.model
Remark 650 HELIX DETERMINATION METHOD: AUTHOR PROVIDED.

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: CREB-BINDING PROTEIN
B: NUCLEAR RECEPTOR COACTIVATOR 1


Theoretical massNumber of molelcules
Total (without water)12,8682
Polymers12,8682
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)37 / -
RepresentativeModel #20

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Components

#1: Protein CREB-BINDING PROTEIN /


Mass: 6568.562 Da / Num. of mol.: 1 / Fragment: SID, RESIDUES 2059-2117
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) MUS MUSCULUS (house mouse) / Plasmid: PET22B / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): B834 / References: UniProt: P45481, histone acetyltransferase
#2: Protein NUCLEAR RECEPTOR COACTIVATOR 1 / / STEROID RECEPTOR COACTIVATOR 1 / NCOA-1 / SRC-1 / RIP160 / HIN-2 PROTEIN


Mass: 6299.123 Da / Num. of mol.: 1 / Fragment: AD1, RESIDUES 920-970
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) HOMO SAPIENS (human) / Plasmid: PET22B / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): B834 / References: UniProt: Q15788, histone acetyltransferase
Compound detailsACETYLATES HISTONES, GIVING A SPECIFIC TAG FOR TRANSCRIPTIONAL ACTIVATION AND NUCLEAR RECEPTOR ...ACETYLATES HISTONES, GIVING A SPECIFIC TAG FOR TRANSCRIPTIONAL ACTIVATION AND NUCLEAR RECEPTOR COACTIVATOR THAT DIRECTLY BINDS NUCLEAR RECEPTORS AND STIMULATES THE TRANSCRIPTIONAL ACTIVITIES

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
11115N-1H HSQC 15N-13C-1H HN(CA)CB 15N-13C-1H CBCA(CO)NH 15N-1H TOCSY-HSQC
22215N-1H NOESYHSQC
33313C-1H HMQCNOESY
44413C-1H HCCHTOCSY NOESY TOCSY
NMR detailsText: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON 13C, 15N-LABELED CBP-SID SRC1-AD1.

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Sample preparation

Details
Solution-IDContents
190% WATER/10% D2O
290% WATER/10% D2O
3100% D2O
4100% D2O
Sample conditions
Conditions-IDIonic strengthpHTemperature (K)
1100 mM7.0 298.0 K
2100 mM7.0 298.0 K
3100 mM7.0 298.0 K
4100 mM7.0 298.0 K

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NMR measurement

NMR spectrometer
TypeManufacturerModelField strength (MHz)Spectrometer-ID
Bruker AvanceBrukerAvance6001
Varian INOVAVarianINOVA8002
Varian INOVAVarianINOVA8003
Bruker AvanceBrukerAvance6004

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Processing

NMR software
NameDeveloperClassification
CYANAGUNTERT,MUMENTHALER,WUTHRICHrefinement
CYANAstructure solution
RefinementMethod: CANDID / Software ordinal: 1
Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE.
NMR ensembleConformers submitted total number: 37

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