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- PDB-1sb0: Solution structure of the KIX domain of CBP bound to the transact... -

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Basic information

Entry
Database: PDB / ID: 1sb0
TitleSolution structure of the KIX domain of CBP bound to the transactivation domain of c-Myb
Components
  • protein CBP
  • protein c-Myb
KeywordsTRANSCRIPTION / CREB-binding protein / transcriptional activation / constitutive activation / LXXLL motif / Myb / KIX
Function / homology
Function and homology information


positive regulation of testosterone secretion / positive regulation of hepatic stellate cell proliferation / Activation of the TFAP2 (AP-2) family of transcription factors / Regulation of FOXO transcriptional activity by acetylation / myeloid cell development / TRAF6 mediated IRF7 activation / Nuclear events mediated by NFE2L2 / Attenuation phase / LRR FLII-interacting protein 1 (LRRFIP1) activates type I IFN production / Regulation of gene expression by Hypoxia-inducible Factor ...positive regulation of testosterone secretion / positive regulation of hepatic stellate cell proliferation / Activation of the TFAP2 (AP-2) family of transcription factors / Regulation of FOXO transcriptional activity by acetylation / myeloid cell development / TRAF6 mediated IRF7 activation / Nuclear events mediated by NFE2L2 / Attenuation phase / LRR FLII-interacting protein 1 (LRRFIP1) activates type I IFN production / Regulation of gene expression by Hypoxia-inducible Factor / RUNX1 regulates transcription of genes involved in differentiation of myeloid cells / Formation of the beta-catenin:TCF transactivating complex / NOTCH1 Intracellular Domain Regulates Transcription / RUNX3 regulates NOTCH signaling / cAMP response element binding protein binding / Notch-HLH transcription pathway / positive regulation of transforming growth factor beta production / Transcriptional and post-translational regulation of MITF-M expression and activity / positive regulation of hepatic stellate cell activation / negative regulation of hematopoietic progenitor cell differentiation / skeletal muscle cell proliferation / germ-line stem cell population maintenance / negative regulation of viral process / Regulation of lipid metabolism by PPARalpha / Cytoprotection by HMOX1 / embryonic digestive tract development / Estrogen-dependent gene expression / CD209 (DC-SIGN) signaling / peptide lactyltransferase (CoA-dependent) activity / outer kinetochore / myeloid cell differentiation / negative regulation of interferon-beta production / N-terminal peptidyl-lysine acetylation / cellular response to interleukin-6 / MRF binding / face morphogenesis / T-helper 2 cell differentiation / negative regulation of transcription by RNA polymerase I / WD40-repeat domain binding / protein-lysine-acetyltransferase activity / stem cell division / cellular response to hepatocyte growth factor stimulus / acetyltransferase activity / histone H3K18 acetyltransferase activity / histone H3K27 acetyltransferase activity / histone acetyltransferase activity / TFIIB-class transcription factor binding / histone acetyltransferase complex / positive regulation of collagen biosynthetic process / homeostasis of number of cells / negative regulation of megakaryocyte differentiation / positive regulation of double-strand break repair via homologous recombination / histone acetyltransferase / positive regulation of DNA-binding transcription factor activity / spleen development / cellular response to retinoic acid / positive regulation of glial cell proliferation / Transferases; Acyltransferases; Transferring groups other than aminoacyl groups / B cell differentiation / thymus development / cellular response to leukemia inhibitory factor / erythrocyte differentiation / response to ischemia / positive regulation of smooth muscle cell proliferation / RNA polymerase II transcription regulatory region sequence-specific DNA binding / G1/S transition of mitotic cell cycle / protein destabilization / PML body / cellular response to virus / positive regulation of miRNA transcription / RNA polymerase II transcription regulator complex / cellular response to hydrogen peroxide / disordered domain specific binding / cellular response to UV / calcium ion transport / rhythmic process / positive regulation of neuron apoptotic process / regulation of gene expression / DNA-binding transcription activator activity, RNA polymerase II-specific / molecular adaptor activity / transcription regulator complex / DNA-binding transcription factor binding / damaged DNA binding / in utero embryonic development / RNA polymerase II-specific DNA-binding transcription factor binding / response to hypoxia / transcription coactivator activity / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / protein domain specific binding / chromatin binding / positive regulation of gene expression / regulation of DNA-templated transcription / chromatin / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / protein-containing complex / DNA binding / zinc ion binding
Similarity search - Function
Coactivator CBP, KIX domain / Transcription regulator Wos2-domain / LMSTEN motif / C-myb, C-terminal / C-myb, C-terminal / : / Nuclear receptor coactivator, CREB-bp-like, interlocking / Nuclear receptor coactivator, CREB-bp-like, interlocking domain superfamily / Creb binding / Zinc finger, TAZ-type ...Coactivator CBP, KIX domain / Transcription regulator Wos2-domain / LMSTEN motif / C-myb, C-terminal / C-myb, C-terminal / : / Nuclear receptor coactivator, CREB-bp-like, interlocking / Nuclear receptor coactivator, CREB-bp-like, interlocking domain superfamily / Creb binding / Zinc finger, TAZ-type / TAZ domain superfamily / TAZ zinc finger / Zinc finger TAZ-type profile. / TAZ zinc finger, present in p300 and CBP / : / Histone acetyltransferase p300-like, PHD domain / Coactivator CBP, KIX domain / CREB-binding protein/p300, atypical RING domain / CBP/p300-type histone acetyltransferase domain / CBP/p300, atypical RING domain superfamily / KIX domain / CREB-binding protein/p300, atypical RING domain / KIX domain profile. / CBP/p300-type histone acetyltransferase (HAT) domain profile. / Histone acetyltransferase Rtt109/CBP / Histone acetylation protein / Histone acetylation protein / Coactivator CBP, KIX domain superfamily / Myb-type HTH DNA-binding domain profile. / Serum Albumin; Chain A, Domain 1 / Zinc finger ZZ-type signature. / Myb domain / Zinc-binding domain, present in Dystrophin, CREB-binding protein. / Zinc finger, ZZ type / Zinc finger, ZZ-type / Zinc finger, ZZ-type superfamily / Zinc finger ZZ-type profile. / Myb-like DNA-binding domain / SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains / SANT/Myb domain / Nuclear receptor coactivator, interlocking / Homeobox-like domain superfamily / Bromodomain, conserved site / Bromodomain signature. / Bromodomain / bromo domain / Bromodomain / Bromodomain (BrD) profile. / Bromodomain-like superfamily / Zinc finger, RING/FYVE/PHD-type / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
Transcriptional activator Myb / Histone lysine acetyltransferase CREBBP
Similarity search - Component
Biological speciesMus musculus (house mouse)
MethodSOLUTION NMR / initial structures from DYANA, refined in AMBER
AuthorsZor, T. / De Guzman, R.N. / Dyson, H.J. / Wright, P.E.
CitationJournal: J.Mol.Biol. / Year: 2004
Title: Solution Structure of the KIX Domain of CBP Bound to the Transactivation Domain of c-Myb
Authors: Zor, T. / De Guzman, R.N. / Dyson, H.J. / Wright, P.E.
History
DepositionFeb 9, 2004Deposition site: RCSB / Processing site: RCSB
Revision 1.0Apr 13, 2004Provider: repository / Type: Initial release
Revision 1.1Apr 29, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Mar 2, 2022Group: Data collection / Database references / Derived calculations
Category: database_2 / pdbx_nmr_software ...database_2 / pdbx_nmr_software / pdbx_nmr_spectrometer / pdbx_struct_assembly / pdbx_struct_oper_list
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_nmr_software.name / _pdbx_nmr_spectrometer.model
Revision 1.4May 22, 2024Group: Data collection / Category: chem_comp_atom / chem_comp_bond

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: protein CBP
B: protein c-Myb


Theoretical massNumber of molelcules
Total (without water)13,3012
Polymers13,3012
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)20 / 200structures with the lowest energy
RepresentativeModel #1lowest energy

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Components

#1: Protein protein CBP / CREB-binding protein


Mass: 10353.856 Da / Num. of mol.: 1 / Fragment: KIX domain
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: CBP / Plasmid: pET / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) DNAY / References: UniProt: P45481
#2: Protein/peptide protein c-Myb / Myb proto-oncogene protein


Mass: 2947.469 Da / Num. of mol.: 1 / Fragment: transcriptional activation domain
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: c-Myb / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) DNAY / References: UniProt: P06876

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
1112D NOESY
1212D TOCSY
1313D 13C-separated NOESY
1413D 15N-separated NOESY

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Sample preparation

DetailsContents: 20 mM Tris d11 acetate d4; 50 mM NaCl; 2 mM NaN3; pH 5.5
Solvent system: 90% H20; 10% D2O
Sample conditionsIonic strength: 50 mM / pH: 5.5 / Pressure: ambient / Temperature: 27 K

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NMR measurement

NMR spectrometer
TypeManufacturerModelField strength (MHz)Spectrometer-ID
Bruker AVANCEBrukerAVANCE6001
Bruker AVANCEBrukerAVANCE8002

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Processing

NMR software
NameVersionDeveloperClassification
DYANA1.5Peter Guntertstructure solution
Amber7David Case et al.refinement
NMRPipeDelaglio, F. et al.processing
NMRView4.3.1Johnson, B.data analysis
RefinementMethod: initial structures from DYANA, refined in AMBER / Software ordinal: 1
NMR representativeSelection criteria: lowest energy
NMR ensembleConformer selection criteria: structures with the lowest energy
Conformers calculated total number: 200 / Conformers submitted total number: 20

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