+Open data
-Basic information
Entry | Database: PDB / ID: 2c0j | ||||||
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Title | Crystal structure of the bet3-trs33 heterodimer | ||||||
Components |
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Keywords | TRANSPORT / TRANSPORT PROTEIN / ENDOPLASMIC RETICULUM / ER-GOLGI TRANSPORT / LIPOPROTEIN / PALMITATE | ||||||
Function / homology | Function and homology information vesicle coating / vesicle tethering / TRAPPII protein complex / TRAPPIII protein complex / TRAPP complex / COPII vesicle coating / intra-Golgi vesicle-mediated transport / RAB GEFs exchange GTP for GDP on RABs / cis-Golgi network / cis-Golgi network membrane ...vesicle coating / vesicle tethering / TRAPPII protein complex / TRAPPIII protein complex / TRAPP complex / COPII vesicle coating / intra-Golgi vesicle-mediated transport / RAB GEFs exchange GTP for GDP on RABs / cis-Golgi network / cis-Golgi network membrane / COPII-mediated vesicle transport / endoplasmic reticulum to Golgi vesicle-mediated transport / trans-Golgi network / Golgi membrane / Golgi apparatus / endoplasmic reticulum / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | HOMO SAPIENS (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / OTHER / Resolution: 2.2 Å | ||||||
Authors | Kim, M.-S. / Yi, M.-J. / Lee, K.-H. / Wagner, J. / Munger, C. / Kim, Y.-G. / Whiteway, M. / Cygler, M. / Oh, B.-H. / Sacher, M. | ||||||
Citation | Journal: Traffic / Year: 2005 Title: Biochemical and Crystallographic Studies Reveal a Specific Interaction between Trapp Subunits Trs33P and Bet3P Authors: Kim, M.-S. / Yi, M.-J. / Lee, K.-H. / Wagner, J. / Munger, C. / Kim, Y.-G. / Whiteway, M. / Cygler, M. / Oh, B.-H. / Sacher, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2c0j.cif.gz | 68.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2c0j.ent.gz | 54.7 KB | Display | PDB format |
PDBx/mmJSON format | 2c0j.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/c0/2c0j ftp://data.pdbj.org/pub/pdb/validation_reports/c0/2c0j | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 18230.748 Da / Num. of mol.: 1 / Fragment: RESIDUES 15-175 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: O43617 |
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#2: Protein | Mass: 17677.539 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: O75865 |
#3: Chemical | ChemComp-PLM / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.31 Å3/Da / Density % sol: 43 % |
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-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: SYNCHROTRON / Site: PAL/PLS / Beamline: 6B / Wavelength: 1 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→20 Å / Num. obs: 15881 / % possible obs: 92.1 % / Observed criterion σ(I): 1 / Redundancy: 3.8 % / Rmerge(I) obs: 0.06 |
-Processing
Software |
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Refinement | Method to determine structure: OTHER / Resolution: 2.2→10 Å / Data cutoff high absF: 10000 / Cross valid method: THROUGHOUT / σ(F): 1
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Solvent computation | Bsol: 47.0909 Å2 / ksol: 0.456723 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.2→10 Å
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Refine LS restraints |
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Xplor file |
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