+Open data
-Basic information
Entry | Database: PDB / ID: 1wgv | ||||||
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Title | Solution Structure of the CS Domain of Human KIAA1068 Protein | ||||||
Components | KIAA1068 protein | ||||||
Keywords | Structural genomics / unknown function / CS domain / HSP20-like fold / KIAA1068 protein / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | Function and homology information protein localization to pericentriolar material / cytoplasmic dynein complex / cilium assembly / unfolded protein binding / protein folding / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Li, H. / Hayashi, F. / Koshiba, S. / Inoue, M. / Kigawa, T. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: To be Published Title: Solution Structure of the CS Domain of Human KIAA1068 Protein Authors: Li, H. / Hayashi, F. / Koshiba, S. / Inoue, M. / Kigawa, T. / Yokoyama, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1wgv.cif.gz | 726.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1wgv.ent.gz | 627.2 KB | Display | PDB format |
PDBx/mmJSON format | 1wgv.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wg/1wgv ftp://data.pdbj.org/pub/pdb/validation_reports/wg/1wgv | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 13537.005 Da / Num. of mol.: 1 / Fragment: CS domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Description: Cell-free protein synthesis / Gene: KAZUSA cDNA hj05437 / Plasmid: P040114-35 / References: UniProt: Q8IVD9 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.06mM CS domain of Human KIAA1068 protein U-13C, 15N; 20mM d-Tris-HCl (pH7.0); 100mM NaCl; 2mM d-DTT; 0.02% NaN3; 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 120mM / pH: 7.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 800 MHz |
-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations, target function Conformers calculated total number: 100 / Conformers submitted total number: 20 |