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Yorodumi- PDB-1v5x: Crystal structure of Phosphoribosyl anthranilate isomerase from T... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1v5x | ||||||
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Title | Crystal structure of Phosphoribosyl anthranilate isomerase from Thermus Thermophilus | ||||||
Components | Phosphoribosylanthranilate isomerase | ||||||
Keywords | ISOMERASE / alpha-beta barrel / TrpF / phosphoribosyl anthranilate isomerase / RIKEN Structural Genomics/Proteomics Initiative / RSGI / Structural Genomics | ||||||
Function / homology | Function and homology information phosphoribosylanthranilate isomerase / phosphoribosylanthranilate isomerase activity / tryptophan biosynthetic process Similarity search - Function | ||||||
Biological species | Thermus thermophilus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||
Authors | Taka, J. / Kunishima, N. / Yutani, K. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: J.Biochem.(Tokyo) / Year: 2005 Title: Stabilization due to dimer formation of phosphoribosyl anthranilate isomerase from Thermus thermophilus HB8: X-ray Analysis and DSC experiments. Authors: Taka, J. / Ogasahara, K. / Jeyakanthan, J. / Kunishima, N. / Kuroishi, C. / Sugahara, M. / Yokoyama, S. / Yutani, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1v5x.cif.gz | 94 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1v5x.ent.gz | 72.6 KB | Display | PDB format |
PDBx/mmJSON format | 1v5x.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/v5/1v5x ftp://data.pdbj.org/pub/pdb/validation_reports/v5/1v5x | HTTPS FTP |
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-Related structure data
Related structure data | 1nsjS S: Starting model for refinement |
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Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Details | The biological assembly is a dimer generated in the asymmetric unit. |
-Components
#1: Protein | Mass: 21977.461 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermus thermophilus (bacteria) / Plasmid: pET11a / Production host: Escherichia coli (E. coli) References: UniProt: P83825, phosphoribosylanthranilate isomerase #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.92 Å3/Da / Density % sol: 57.52 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: microbatch / pH: 5.1 Details: Isopropanol, Sodium Acetate, pH 5.1, microbatch, temperature 293K | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 22 ℃ / pH: 8 / Method: batch method | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SPring-8 / Beamline: BL26B1 / Wavelength: 1 Å |
Detector | Type: RIGAKU RAXIS V / Detector: IMAGE PLATE / Date: Oct 3, 2003 / Details: mirrors |
Radiation | Monochromator: Si 111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2→35 Å / Num. all: 35156 / Num. obs: 35156 / % possible obs: 99.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 8.7 % / Biso Wilson estimate: 29.43 Å2 / Rmerge(I) obs: 0.08 / Rsym value: 0.076 / Net I/σ(I): 9.92 |
Reflection shell | Resolution: 2→2.07 Å / Redundancy: 5 % / Rmerge(I) obs: 0.763 / Mean I/σ(I) obs: 3 / Num. unique all: 3404 / Rsym value: 0.698 / % possible all: 99.4 |
Reflection | *PLUS Num. measured all: 307402 / Rmerge(I) obs: 0.08 |
Reflection shell | *PLUS % possible obs: 99.4 % |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB entry 1NSJ Resolution: 2→35 Å / Isotropic thermal model: Anisotropic / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 44.9 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2→35 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2→2.09 Å / Rfactor Rfree error: 0.026
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Refinement | *PLUS % reflection Rfree: 5 % | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS |