+Open data
-Basic information
Entry | Database: PDB / ID: 1t1l | ||||||
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Title | Crystal structure of the long-chain fatty acid transporter FadL | ||||||
Components | Long-chain fatty acid transport protein | ||||||
Keywords | LIPID TRANSPORT / beta-barrel / hatch domain | ||||||
Function / homology | Function and homology information long-chain fatty acid transporting porin activity / ligand-gated channel activity / cell outer membrane Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MIR / Resolution: 2.8 Å | ||||||
Authors | van den Berg, B. / Black, P.N. / Clemons Jr., W.M. / Rapoport, T.A. | ||||||
Citation | Journal: Science / Year: 2004 Title: Crystal structure of the long-chain fatty acid transporter FadL. Authors: van den Berg, B. / Black, P.N. / Clemons Jr., W.M. / Rapoport, T.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1t1l.cif.gz | 171.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1t1l.ent.gz | 136.7 KB | Display | PDB format |
PDBx/mmJSON format | 1t1l.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/t1/1t1l ftp://data.pdbj.org/pub/pdb/validation_reports/t1/1t1l | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 46766.996 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: FADL, TTR, B2344 / Plasmid: pBAD22 / Production host: Escherichia coli (E. coli) / Strain (production host): C43(DE3) / References: UniProt: P10384 #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.8 Å3/Da / Density % sol: 67.8 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 6.8 Details: Ammonium Formate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
-Data collection
Diffraction |
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Diffraction source |
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Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Nov 8, 2003 | |||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
Radiation wavelength |
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Reflection | Resolution: 2.7→49.03 Å / Num. all: 39365 / Num. obs: 36854 / % possible obs: 93.6 % / Observed criterion σ(F): 2 / Redundancy: 3.3 % / Biso Wilson estimate: 32.3 Å2 / Limit h max: 39 / Limit h min: 0 / Limit k max: 39 / Limit k min: 0 / Limit l max: 60 / Limit l min: 0 / Observed criterion F max: 149824.9 / Observed criterion F min: 0.32 / Rsym value: 0.07 | |||||||||||||||
Reflection shell | Resolution: 2.7→2.8 Å / Redundancy: 2.7 % / Rsym value: 0.306 |
-Processing
Software |
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Refinement | Method to determine structure: MIR / Resolution: 2.8→11.99 Å / Rfactor Rfree error: 0.008 / Occupancy max: 1 / Occupancy min: 1 / Cross valid method: THROUGHOUT / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: CNS bulk solvent model used / Bsol: 45.5888 Å2 / ksol: 0.294514 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 92.74 Å2 / Biso mean: 43.01 Å2 / Biso min: 18.13 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.8→11.99 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 8
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Xplor file |
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