+Open data
-Basic information
Entry | Database: PDB / ID: 1sbt | |||||||||
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Title | ATOMIC COORDINATES FOR SUBTILISIN BPN (OR NOVO) | |||||||||
Components | SUBTILISIN BPN' | |||||||||
Keywords | HYDROLASE (SERINE PROTEINASE) | |||||||||
Function / homology | Function and homology information subtilisin / sporulation resulting in formation of a cellular spore / fibrinolysis / serine-type endopeptidase activity / proteolysis / extracellular space / metal ion binding Similarity search - Function | |||||||||
Biological species | Bacillus amyloliquefaciens (bacteria) | |||||||||
Method | X-RAY DIFFRACTION / Resolution: 2.5 Å | |||||||||
Authors | Alden, R.A. / Birktoft, J.J. / Kraut, J. / Robertus, J.D. / Wright, C.S. | |||||||||
Citation | Journal: Biochem.Biophys.Res.Commun. / Year: 1971 Title: Atomic coordinates for subtilisin BPN' (or Novo). Authors: Alden, R.A. / Birktoft, J.J. / Kraut, J. / Robertus, J.D. / Wright, C.S. #1: Journal: J.Biol.Chem. / Year: 1976 Title: Polypeptide Halomethyl Ketones Bind to Serine Proteases as Analogs of the Tetrahedral Intermediate,X-Ray Crystallographic Comparison of Lysine-and Phenylalanine-Polypeptide Chloromethyl Ketone-Inhibited Subtilisin Authors: Poulos, T.L. / Alden, R.A. / Freer, S.T. / Birktoft, J.J. / Kraut, J. #2: Journal: J.Biol.Chem. / Year: 1975 Title: X-Ray Crystallographic Study of Boronic Acid Adducts with Subtilisin Bpn(Novo),A Model for the Catalytic Transition State Authors: Matthews, D.A. / Alden, R.A. / Birktoft, J.J. / Freer, S.T. / Kraut, J. #3: Journal: Biochemistry / Year: 1972 Title: Subtilisin,A Stereochemical Mechanism Involving Transition-State Stabilization Authors: Robertus, J.D. / Kraut, J. / Alden, R.A. / Birktoft, J.J. #4: Journal: Biochemistry / Year: 1972 Title: An X-Ray Crystallographic Study of the Binding of Peptide Chloromethyl Ketone Inhibitors to Subtilisin Bpn Authors: Robertus, J.D. / Alden, R.A. / Birktoft, J.J. / Kraut, J. / Powers, J.C. / Wilcox, P.E. #5: Journal: Cold Spring Harbor Symp.Quant.Biol. / Year: 1972 Title: The Aromatic Substrate Binding Site in Subtilisin Bpnand its Resemblance to Chymotrypsin Authors: Kraut, J. / Robertus, J.D. / Birktoft, J.J. / Alden, R.A. / Wilcox, P.E. / Powers, J.C. #6: Journal: Biochem.Biophys.Res.Commun. / Year: 1971 Title: On the Identity of Subtilisins Bpnand Novo Authors: Robertus, J.D. / Alden, R.A. / Kraut, J. #7: Journal: Philos.Trans.R.Soc.London,Ser.B / Year: 1970 Title: A Hydrogen-Bond Network at the Active Site of Subtilisin Bpn Authors: Alden, R.A. / Wright, C.S. / Kraut, J. #8: Journal: Nature / Year: 1969 Title: Structure of Subtilisin Bpnat 2.5 Angstroms Resolution Authors: Wright, C.S. / Alden, R.A. / Kraut, J. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1sbt.cif.gz | 55.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1sbt.ent.gz | 32.4 KB | Display | PDB format |
PDBx/mmJSON format | 1sbt.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1sbt_validation.pdf.gz | 373.6 KB | Display | wwPDB validaton report |
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Full document | 1sbt_full_validation.pdf.gz | 474.9 KB | Display | |
Data in XML | 1sbt_validation.xml.gz | 20.3 KB | Display | |
Data in CIF | 1sbt_validation.cif.gz | 26.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sb/1sbt ftp://data.pdbj.org/pub/pdb/validation_reports/sb/1sbt | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: THE FO-FC MAP INDICATES COORDINATE ERRORS OF MORE THAN 1 ANGSTROM. 2: THESE COORDINATES ARE THE OUTPUT OF A REFINEMENT BY DIAMOND'S MODEL-BUILDING PROGRAM. |
-Components
#1: Protein | Mass: 27552.525 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus amyloliquefaciens (bacteria) / References: UniProt: P00782, 3.4.21.14 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.07 Å3/Da / Density % sol: 40.54 % |
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Crystal grow | *PLUS Method: other / Details: Wright, C.S., (1969) Nature, 221, 235. |
-Processing
Refinement | Highest resolution: 2.5 Å Details: THE FO-FC MAP INDICATES COORDINATE ERRORS OF MORE THAN 1 ANGSTROM. THESE COORDINATES ARE THE OUTPUT OF A REFINEMENT BY DIAMOND'S MODEL-BUILDING PROGRAM | ||||||||||||
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Refinement step | Cycle: LAST / Highest resolution: 2.5 Å
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