+Open data
-Basic information
Entry | Database: PDB / ID: 1r2z | ||||||
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Title | MutM (Fpg) bound to 5,6-dihydrouracil (DHU) containing DNA | ||||||
Components |
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Keywords | HYDROLASE/DNA / DNA REPAIR / DNA GLYCOSYLASE / HYDROLASE-DNA COMPLEX | ||||||
Function / homology | Function and homology information DNA-formamidopyrimidine glycosylase / oxidized purine nucleobase lesion DNA N-glycosylase activity / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / DNA-(apurinic or apyrimidinic site) lyase / base-excision repair / damaged DNA binding / zinc ion binding Similarity search - Function | ||||||
Biological species | Geobacillus stearothermophilus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / FOURIER SYNTHESIS / Resolution: 1.63 Å | ||||||
Authors | Fromme, J.C. / Verdine, G.L. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2003 Title: DNA Lesion Recognition by the Bacterial Repair Enzyme MutM. Authors: Fromme, J.C. / Verdine, G.L. | ||||||
History |
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Remark 999 | SEQUENCE The sequence of this protein is not available in any reference sequence database. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1r2z.cif.gz | 89.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1r2z.ent.gz | 63.1 KB | Display | PDB format |
PDBx/mmJSON format | 1r2z.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/r2/1r2z ftp://data.pdbj.org/pub/pdb/validation_reports/r2/1r2z | HTTPS FTP |
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-Related structure data
Related structure data | 1r2yC 1l1tS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: DNA chain | Mass: 3687.417 Da / Num. of mol.: 1 / Source method: obtained synthetically |
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#2: DNA chain | Mass: 3599.340 Da / Num. of mol.: 1 / Source method: obtained synthetically |
#3: Protein | Mass: 30738.525 Da / Num. of mol.: 1 / Mutation: E3Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) Geobacillus stearothermophilus (bacteria) Gene: mutm / Plasmid: pET24b / Production host: Escherichia coli (E. coli) / References: UniProt: P84131 |
#4: Chemical | ChemComp-ZN / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.8 Å3/Da / Density % sol: 56.05 % | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: PEG 8000, MAGNESIUM ACETATE, SODIUM CACODYLATE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS Method: vapor diffusion / Details: Fromme, J.C., (2002) Nat.Struct.Biol., 9, 544. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X25 / Wavelength: 1.1 Å |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jun 30, 2003 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1 Å / Relative weight: 1 |
Reflection | Resolution: 1.63→50 Å / Num. all: 54565 / Num. obs: 53944 / % possible obs: 98.9 % / Observed criterion σ(I): -3 / Redundancy: 5.3 % / Biso Wilson estimate: 19.3 Å2 / Rmerge(I) obs: 0.048 / Net I/σ(I): 31 |
Reflection shell | Resolution: 1.63→1.69 Å / Redundancy: 4.8 % / Rmerge(I) obs: 0.357 / Mean I/σ(I) obs: 4 / Num. unique all: 5369 / % possible all: 93.9 |
Reflection | *PLUS Lowest resolution: 50 Å |
Reflection shell | *PLUS % possible obs: 93.9 % |
-Processing
Software |
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Refinement | Method to determine structure: FOURIER SYNTHESIS Starting model: PDB entry 1L1T Resolution: 1.63→50 Å / Rfactor Rfree error: 0.005 / Data cutoff high absF: 314184.8 / Data cutoff high rms absF: 314184.8 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 49.7263 Å2 / ksol: 0.372355 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 29.5 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.63→50 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.63→1.69 Å / Rfactor Rfree error: 0.016 / Total num. of bins used: 10
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Xplor file |
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Refinement | *PLUS Lowest resolution: 50 Å | ||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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