+Open data
-Basic information
Entry | Database: PDB / ID: 1obp | ||||||
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Title | ODORANT-BINDING PROTEIN FROM BOVINE NASAL MUCOSA | ||||||
Components | ODORANT-BINDING PROTEIN | ||||||
Keywords | ODORANT-BINDING PROTEIN / OLFACTION / NOSE / TRANSPORT / LIPOCALIN | ||||||
Function / homology | Function and homology information odorant binding / small molecule binding / sensory perception of smell / extracellular space Similarity search - Function | ||||||
Biological species | Bos taurus (cattle) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2 Å | ||||||
Authors | Tegoni, M. / Cambillau, C. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 1996 Title: Domain swapping creates a third putative combining site in bovine odorant binding protein dimer Authors: Tegoni, M. / Ramoni, R. / Bignetti, E. / Spinelli, S. / Cambillau, C. #1: Journal: J.Biotechnol. / Year: 1993 Title: Preparation of an Affinity Resin for Odorants by Coupling Odorant Binding Protein from Bovine Nasal Mucosa to Sepharose 4B Authors: Bignetti, E. / Bussolati, L. / Ramoni, R. / Grolli, S. / Donofrio, G. #2: Journal: Eur.J.Biochem. / Year: 1985 Title: Purification and Characterisation of an Odorant-Binding Protein from Cow Nasal Tissue Authors: Bignetti, E. / Cavaggioni, A. / Pelosi, P. / Persaud, K.C. / Sorbi, R.T. / Tirindelli, R. #3: Journal: Biochem.J. / Year: 1982 Title: Identification of a Specific Olfactory Receptor for 2-Isobutyl-3-Methoxypyrazine Authors: Pelosi, P. / Baldaccini, N.E. / Pisanelli, A.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1obp.cif.gz | 116 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1obp.ent.gz | 85 KB | Display | PDB format |
PDBx/mmJSON format | 1obp.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1obp_validation.pdf.gz | 405.4 KB | Display | wwPDB validaton report |
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Full document | 1obp_full_validation.pdf.gz | 422.3 KB | Display | |
Data in XML | 1obp_validation.xml.gz | 11.6 KB | Display | |
Data in CIF | 1obp_validation.cif.gz | 17.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ob/1obp ftp://data.pdbj.org/pub/pdb/validation_reports/ob/1obp | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.294, -0.342, -0.893), Vector: |
-Components
#1: Protein | Mass: 18457.305 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Bos taurus (cattle) / Organ: NOSE / Tissue: RESPIRATORY EPITHELIUM / References: UniProt: P07435 #2: Chemical | ChemComp-UNX / #3: Water | ChemComp-HOH / | Nonpolymer details | RESIDUES NAMED UNX ARE PSEUDO CARBON ATOMS USED TO MODEL AN UNKNOWN LIGAND. TO ALLOW ATOM OVERLAP, ...RESIDUES NAMED UNX ARE PSEUDO CARBON ATOMS USED TO MODEL AN UNKNOWN LIGAND. TO ALLOW ATOM OVERLAP, SCATTERING | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.09 Å3/Da / Density % sol: 40 % |
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Crystal grow | pH: 5.7 / Details: pH 5.7 |
Crystal grow | *PLUS Method: unknown |
Components of the solutions | *PLUS Conc.: 35-45 % / Common name: ethanol |
-Data collection
Diffraction | Mean temperature: 289 K |
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Diffraction source | Source: ROTATING ANODE / Wavelength: 1.5418 |
Detector | Type: MAR scanner 180 mm plate / Detector: IMAGE PLATE / Date: May 26, 1995 |
Radiation | Monochromator: MONOCHROMATOR / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2→20 Å / Num. obs: 20232 / % possible obs: 94.6 % / Observed criterion σ(I): 1 / Redundancy: 3 % / Rmerge(I) obs: 0.045 |
-Processing
Software |
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Refinement | Resolution: 2→6 Å / σ(F): 1
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Displacement parameters | Biso mean: 30.5 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→6 Å
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Refine LS restraints |
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