[English] 日本語
Yorodumi- PDB-1mst: CRYSTAL STRUCTURE OF MS2 CAPSIDS WITH MUTATIONS IN THE SUBUNIT FG LOOP -
+Open data
-Basic information
Entry | Database: PDB / ID: 1mst | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | CRYSTAL STRUCTURE OF MS2 CAPSIDS WITH MUTATIONS IN THE SUBUNIT FG LOOP | |||||||||
Components | BACTERIOPHAGE MS2 CAPSID | |||||||||
Keywords | VIRUS / BACTERIOPHAGE COAT PROTEIN / Icosahedral virus | |||||||||
Function / homology | Function and homology information negative regulation of viral translation / T=3 icosahedral viral capsid / regulation of translation / structural molecule activity / RNA binding / identical protein binding Similarity search - Function | |||||||||
Biological species | Enterobacterio phage MS2 (virus) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2.6 Å | |||||||||
Authors | Liljas, L. / Stonehouse, N.J. | |||||||||
Citation | Journal: J.Mol.Biol. / Year: 1996 Title: Crystal structures of MS2 capsids with mutations in the subunit FG loop. Authors: Stonehouse, N.J. / Valegard, K. / Golmohammadi, R. / van den Worm, S. / Walton, C. / Stockley, P.G. / Liljas, L. #1: Journal: Acta Crystallogr.,Sect.B / Year: 1991 Title: Structure Determination of the Bacteriophage MS2 Authors: Valegard, K. / Liljas, L. / Fridborg, K. / Unge, T. #2: Journal: Nature / Year: 1990 Title: The Three-Dimensional Structure of the Bacterial Virus MS2 Authors: Valegard, K. / Liljas, L. / Fridborg, K. / Unge, T. #3: Journal: Semin.Virol. / Year: 1990 Title: The Structure of Bacteriophage MS2 Authors: Liljas, L. / Valegard, K. #4: Journal: J.Mol.Biol. / Year: 1986 Title: Purification, Crystallization and Preliminary X-Ray Data of the Bacteriophage MS2 Authors: Valegard, K. / Unge, T. / Montelius, I. / Strandberg, B. / Fiers, W. | |||||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1mst.cif.gz | 84.5 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1mst.ent.gz | 68 KB | Display | PDB format |
PDBx/mmJSON format | 1mst.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ms/1mst ftp://data.pdbj.org/pub/pdb/validation_reports/ms/1mst | HTTPS FTP |
---|
-Related structure data
-Links
-Assembly
Deposited unit |
| |||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 |
| x 60|||||||||||||||||||||||||||||||||
2 |
| |||||||||||||||||||||||||||||||||
3 |
| x 5|||||||||||||||||||||||||||||||||
4 |
| x 6|||||||||||||||||||||||||||||||||
5 |
| |||||||||||||||||||||||||||||||||
6 |
| x 10|||||||||||||||||||||||||||||||||
Unit cell |
| |||||||||||||||||||||||||||||||||
Atom site foot note | 1: CIS PROLINE - PRO B 78 | |||||||||||||||||||||||||||||||||
Symmetry | Point symmetry: (Hermann–Mauguin notation: 532 / Schoenflies symbol: I (icosahedral)) | |||||||||||||||||||||||||||||||||
Noncrystallographic symmetry (NCS) | NCS oper:
|
-Components
#1: Protein | Mass: 13724.438 Da / Num. of mol.: 3 / Mutation: E76D Source method: isolated from a genetically manipulated source Source: (gene. exp.) Enterobacterio phage MS2 (virus) / Genus: LevivirusEmesvirus / Species: Enterobacteria phage MS2Bacteriophage MS2 / Production host: Escherichia coli (E. coli) / References: UniProt: P03612 #2: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal grow | *PLUS Temperature: 37 ℃ / pH: 7.4 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Components of the solutions | *PLUS
|
-Data collection
Diffraction source | Source: SYNCHROTRON / Site: SRS / Beamline: PX9.6 / Wavelength: 0.87,0.93,0.95 | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE | ||||||||||||
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
| ||||||||||||
Reflection | Num. obs: 247689 / Rmerge(I) obs: 0.115 | ||||||||||||
Reflection | *PLUS Highest resolution: 2.6 Å / Lowest resolution: 10.59 Å / % possible obs: 79 % / Rmerge(I) obs: 0.115 | ||||||||||||
Reflection shell | *PLUS Highest resolution: 2.6 Å / Lowest resolution: 2.67 Å / % possible obs: 47 % / Num. unique obs: 10886 / Rmerge(I) obs: 0.313 |
-Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Resolution: 2.6→15 Å / σ(F): 2 /
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.6→15 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |