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Yorodumi- PDB-1lv0: Crystal structure of the Rab effector guanine nucleotide dissocia... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1lv0 | ||||||
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Title | Crystal structure of the Rab effector guanine nucleotide dissociation inhibitor (GDI) in complex with a geranylgeranyl (GG) peptide | ||||||
Components | RAB GDP disossociation inhibitor alpha | ||||||
Keywords | SIGNALING PROTEIN / Protein-ligand complex | ||||||
Function / homology | Function and homology information RAB GEFs exchange GTP for GDP on RABs / Rab GDP-dissociation inhibitor activity / negative regulation of protein targeting to membrane / Rab protein signal transduction / negative regulation of axonogenesis / vesicle-mediated transport / GTPase activator activity / protein transport / Golgi apparatus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Bos taurus (cattle) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||
Authors | An, Y. / Shao, Y. / Alory, C. / Matteson, J. / Sakisaka, T. / Chen, W. / Gibbs, R.A. / Wilson, I.A. / Balch, W.E. | ||||||
Citation | Journal: Structure / Year: 2003 Title: Geranylgeranyl switching regulates GDI-Rab GTPase recycling. Authors: An, Y. / Shao, Y. / Alory, C. / Matteson, J. / Sakisaka, T. / Chen, W. / Gibbs, R.A. / Wilson, I.A. / Balch, W.E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1lv0.cif.gz | 108.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1lv0.ent.gz | 81.3 KB | Display | PDB format |
PDBx/mmJSON format | 1lv0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lv/1lv0 ftp://data.pdbj.org/pub/pdb/validation_reports/lv/1lv0 | HTTPS FTP |
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-Related structure data
Related structure data | 1d5tS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 50896.754 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bos taurus (cattle) / Plasmid: PET-11DHIS / Production host: Escherichia coli (E. coli) / References: UniProt: P21856 | ||||
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#2: Chemical | #3: Chemical | ChemComp-GER / | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.27 Å3/Da / Density % sol: 45.79 % |
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Crystal grow | Temperature: 296 K / Method: vapor diffusion, sitting drop / pH: 6.2 Details: Ammonium Sulfate, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
Crystal grow | *PLUS Temperature: 22.5 ℃ / pH: 6.25 / Method: vapor diffusion, sitting dropDetails: Luan, P., (2000) Traffic, 1, 270., Schalk, I., (1996) Nature,381, 42. |
Components of the solutions | *PLUS Conc.: 1.73 M / Common name: ammonium sulfate |
-Data collection
Diffraction | Mean temperature: 98 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL9-2 / Wavelength: 1.033 Å |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Feb 12, 2000 |
Radiation | Monochromator: Mirror / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.033 Å / Relative weight: 1 |
Reflection | Resolution: 2→20 Å / Num. all: 30213 / Num. obs: 30213 / % possible obs: 95.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 2.3 % / Biso Wilson estimate: 12.7 Å2 / Rmerge(I) obs: 0.061 / Rsym value: 0.061 / Net I/σ(I): 12.7 |
Reflection shell | Resolution: 2→2.03 Å / Rmerge(I) obs: 0.419 / Mean I/σ(I) obs: 1.4 / Num. unique all: 1325 / Rsym value: 0.419 / % possible all: 85.9 |
Reflection | *PLUS Num. measured all: 68840 |
Reflection shell | *PLUS % possible obs: 85.9 % |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1D5T Resolution: 2→19.96 Å / Rfactor Rfree error: 0.005 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 54.4971 Å2 / ksol: 0.342343 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 29.2 Å2
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Refine analyze | Luzzati coordinate error free: 0.3 Å / Luzzati sigma a free: 0.32 Å | ||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→19.96 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2→2.03 Å / Rfactor Rfree error: 0.032 / Total num. of bins used: 20
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Xplor file |
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Refinement | *PLUS Lowest resolution: 20 Å / % reflection Rfree: 5 % | ||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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