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Yorodumi- PDB-1hv2: SOLUTION STRUCTURE OF YEAST ELONGIN C IN COMPLEX WITH A VON HIPPE... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1hv2 | ||||||
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Title | SOLUTION STRUCTURE OF YEAST ELONGIN C IN COMPLEX WITH A VON HIPPEL-LINDAU PEPTIDE | ||||||
Components |
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Keywords | SIGNALING PROTEIN / protein-peptide complex | ||||||
Function / homology | Function and homology information regulation of catecholamine metabolic process / homeostasis of number of retina cells / : / negative regulation of hypoxia-inducible factor-1alpha signaling pathway / regulation of protein localization => GO:0032880 / pancreatic A cell differentiation / nucleotide-excision repair factor 4 complex / type B pancreatic cell differentiation / SUMOylation of ubiquitinylation proteins / regulation of thymocyte apoptotic process ...regulation of catecholamine metabolic process / homeostasis of number of retina cells / : / negative regulation of hypoxia-inducible factor-1alpha signaling pathway / regulation of protein localization => GO:0032880 / pancreatic A cell differentiation / nucleotide-excision repair factor 4 complex / type B pancreatic cell differentiation / SUMOylation of ubiquitinylation proteins / regulation of thymocyte apoptotic process / ciliary body morphogenesis / iris morphogenesis / eye pigmentation / negative regulation of endothelial cell differentiation / melanin metabolic process / Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha / global genome nucleotide-excision repair / regulation of protein catabolic process at postsynapse, modulating synaptic transmission / blood vessel endothelial cell migration / camera-type eye morphogenesis / Neddylation / Antigen processing: Ubiquitination & Proteasome degradation / negative regulation of receptor signaling pathway via JAK-STAT / regulation of apoptotic signaling pathway / elongin complex / VCB complex / negative regulation of thymocyte apoptotic process / Cul2-RING ubiquitin ligase complex / Cul3-RING ubiquitin ligase complex / TP53 Regulates Transcription of DNA Repair Genes / transcription factor binding / ubiquitin-like ligase-substrate adaptor activity / response to UV / extracellular matrix organization / positive regulation of epithelial cell proliferation / proteasomal protein catabolic process / nucleotide-excision repair / protein catabolic process / cilium / neuron differentiation / protein-macromolecule adaptor activity / protein transport / ubiquitin-dependent protein catabolic process / postsynapse / regulation of gene expression / angiogenesis / response to ethanol / response to hypoxia / protein ubiquitination / negative regulation of cell population proliferation / negative regulation of gene expression / glutamatergic synapse / protein-containing complex binding / nucleolus / regulation of DNA-templated transcription / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / enzyme binding / mitochondrion / nucleoplasm / membrane / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | SOLUTION NMR / distance geometry, simulating annealing | ||||||
Authors | Botuyan, M.V. / Mer, G. / Yi, G.-S. / Koth, C.M. / Case, D.A. / Edwards, A.M. / Chazin, W.J. / Arrowsmith, C.H. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2001 Title: Solution structure and dynamics of yeast elongin C in complex with a von Hippel-Lindau peptide. Authors: Botuyan, M.V. / Mer, G. / Yi, G.S. / Koth, C.M. / Case, D.A. / Edwards, A.M. / Chazin, W.J. / Arrowsmith, C.H. #1: Journal: Proc.Natl.Acad.Sci.USA / Year: 1999 Title: Binding of Elongin A or a von Hippel-Lindau Peptide Stabilizes the Structure of Yeast Elongin C Authors: Botuyan, M.V. / Koth, C.M. / Mer, G. / Chakrabartty, A. / Conaway, J.W. / Conaway, R.C. / Edwards, A.M. / Arrowsmith, C.H. / Chazin, W.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1hv2.cif.gz | 708.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1hv2.ent.gz | 620.7 KB | Display | PDB format |
PDBx/mmJSON format | 1hv2.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hv/1hv2 ftp://data.pdbj.org/pub/pdb/validation_reports/hv/1hv2 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 11338.760 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Production host: Escherichia coli (E. coli) / References: UniProt: Q03071 |
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#2: Protein/peptide | Mass: 1776.195 Da / Num. of mol.: 1 / Fragment: RESIDUES 157-171 / Source method: obtained synthetically / Details: This peptide was chemically synthesized / References: UniProt: P40338 |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: The structure was determined using triple-resonance NMR spectroscopy |
-Sample preparation
Details |
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Sample conditions | pH: 7.5 / Pressure: ambient / Temperature: 303 K | |||||||||
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||||||||||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||||||||||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: distance geometry, simulating annealing / Software ordinal: 1 | ||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: The submitted conformer models are the 20 structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |