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Yorodumi- PDB-1h4x: Structure of the Bacillus Cell Fate Determinant SpoIIAA in the Ph... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1h4x | ||||||
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Title | Structure of the Bacillus Cell Fate Determinant SpoIIAA in the Phosphorylated Form | ||||||
Components | ANTI-SIGMA F FACTOR ANTAGONIST | ||||||
Keywords | CELL DIFFERENTIATION / PHOSPHORYLATION / SIGMA FACTOR / SPORULATION | ||||||
Function / homology | Function and homology information anti-sigma factor antagonist activity / antisigma factor binding / sporulation resulting in formation of a cellular spore Similarity search - Function | ||||||
Biological species | BACILLUS SPHAERICUS (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.16 Å | ||||||
Authors | Seavers, P.R. / Lewis, R.J. / Brannigan, J.A. / Verschueren, K.H.G. / Murshudov, G.N. / Wilkinson, A.J. | ||||||
Citation | Journal: Structure / Year: 2001 Title: Structure of the Bacillus Cell Fate Determinant Spoiiaa in Phosphorylated and Unphosphorylated Forms Authors: Seavers, P.R. / Lewis, R.J. / Brannigan, J.A. / Verschueren, K.H.G. / Murshudov, G.N. / Wilkinson, A.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1h4x.cif.gz | 64.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1h4x.ent.gz | 52.3 KB | Display | PDB format |
PDBx/mmJSON format | 1h4x.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1h4x_validation.pdf.gz | 440.3 KB | Display | wwPDB validaton report |
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Full document | 1h4x_full_validation.pdf.gz | 447.7 KB | Display | |
Data in XML | 1h4x_validation.xml.gz | 9.6 KB | Display | |
Data in CIF | 1h4x_validation.cif.gz | 14.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/h4/1h4x ftp://data.pdbj.org/pub/pdb/validation_reports/h4/1h4x | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 13205.202 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) BACILLUS SPHAERICUS (bacteria) / Strain: ATCC14577 / Plasmid: PET28A / Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: O32723*PLUS #2: Chemical | #3: Water | ChemComp-HOH / | Compound details | IN THE PHOSPHORYL | Sequence details | THE SEQUENCE SPOIIA FROM BACILLUS SPHAERICUSS STRAIN ATCC14577, IS SIMILAR TO THE SWISSPROT ENTRY ...THE SEQUENCE SPOIIA FROM BACILLUS SPHAERICUS | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.96 Å3/Da / Density % sol: 37.35 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 6.5 Details: 30% PEG 5K MME, 200MM (NH4)2SO4, 100MM MES (PH 6.5) | ||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 291 K / pH: 8.5 / Method: vapor diffusion, hanging drop / Details: Seavers, P.R., (2001) Acta Crystallogr., D57, 292. | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID14-1 / Wavelength: 0.934 |
Detector | Type: ADSC CCD / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.934 Å / Relative weight: 1 |
Reflection | Resolution: 1.16→20 Å / Num. obs: 72227 / % possible obs: 97.9 % / Redundancy: 3.8 % / Rmerge(I) obs: 0.068 / Net I/σ(I): 18.9 |
Reflection shell | Resolution: 1.16→1.2 Å / Redundancy: 2.5 % / Rmerge(I) obs: 0.3 / Mean I/σ(I) obs: 3.72 / % possible all: 85.4 |
Reflection | *PLUS Num. measured all: 277544 |
Reflection shell | *PLUS % possible obs: 85.4 % / Rmerge(I) obs: 0.3 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.16→19.61 Å / Cor.coef. Fo:Fc: 0.979 / Cor.coef. Fo:Fc free: 0.971 / SU B: 1.218 / SU ML: 0.03 / Cross valid method: THROUGHOUT / ESU R: 0.034 / ESU R Free: 0.035 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL PLUS MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.16→19.61 Å
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