[English] 日本語
![](img/lk-miru.gif)
- PDB-1h4z: Structure of the Anti-Sigma Factor Antagonist SpoIIAA in its Unph... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1h4z | ||||||
---|---|---|---|---|---|---|---|
Title | Structure of the Anti-Sigma Factor Antagonist SpoIIAA in its Unphosphorylated Form | ||||||
![]() | ANTI-SIGMA F FACTOR ANTAGONIST | ||||||
![]() | CELL DIFFERENTIATION / PHOSPHORYLATION / SIGMA FACTOR / SPORULATION | ||||||
Function / homology | ![]() anti-sigma factor antagonist activity / antisigma factor binding / sporulation resulting in formation of a cellular spore Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Seavers, P.R. / Lewis, R.J. / Brannigan, J.A. / Verschueren, K.H.G. / Murshudov, G.N. / Wilkinson, A.J. | ||||||
![]() | ![]() Title: Structure of the Bacillus Cell Fate Determinant Spoiiaa in Phosphorylated and Unphosphorylated Forms Authors: Seavers, P.R. / Lewis, R.J. / Brannigan, J.A. / Verschueren, K.H.G. / Murshudov, G.N. / Wilkinson, A.J. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 33.3 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 22.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 419.9 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 426.6 KB | Display | |
Data in XML | ![]() | 7.4 KB | Display | |
Data in CIF | ![]() | 8.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1h4xSC ![]() 1h4yC S: Starting model for refinement C: citing same article ( |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 13125.223 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
---|---|
#2: Water | ChemComp-HOH / |
Sequence details | THE SEQUENCE SPOIIA FROM BACILLUS SPHAERICUSS STRAIN ATCC14577, IS SIMILAR TO THE SWISSPROT ENTRY ...THE SEQUENCE SPOIIA FROM BACILLUS SPHAERICUS |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.62 Å3/Da / Density % sol: 53.07 % | ||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | pH: 9 / Details: 2M MGCL2, 0.1M BICINE (PH 9.0) | ||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 291 K / pH: 8.5 / Method: vapor diffusion, hanging drop / Details: Seavers, P.R., (2001) Acta Crystallogr., D57, 292. | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.93 Å / Relative weight: 1 |
Reflection | Resolution: 2.74→20 Å / Num. obs: 4022 / % possible obs: 99.8 % / Redundancy: 6.7 % / Rmerge(I) obs: 0.072 / Net I/σ(I): 23.5 |
Reflection shell | Resolution: 2.74→2.84 Å / Rmerge(I) obs: 0.342 / Mean I/σ(I) obs: 5.3 / % possible all: 99.7 |
Reflection | *PLUS Num. measured all: 26991 |
Reflection shell | *PLUS % possible obs: 99.7 % |
-
Processing
Software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: 1H4X Resolution: 2.74→20 Å / SU B: 14.33 / SU ML: 0.301 / Cross valid method: THROUGHOUT / ESU R: 1.068 / ESU R Free: 0.404 / Details: NONE
| ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.74→20 Å
| ||||||||||||||||||||
Software | *PLUS Name: REFMAC / Classification: refinement | ||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.25 / Rfactor Rfree: 0.305 / Rfactor Rwork: 0.251 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS
|