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- PDB-1h4x: Structure of the Bacillus Cell Fate Determinant SpoIIAA in the Ph... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1h4x | ||||||
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Title | Structure of the Bacillus Cell Fate Determinant SpoIIAA in the Phosphorylated Form | ||||||
![]() | ANTI-SIGMA F FACTOR ANTAGONIST | ||||||
![]() | CELL DIFFERENTIATION / PHOSPHORYLATION / SIGMA FACTOR / SPORULATION | ||||||
Function / homology | ![]() anti-sigma factor antagonist activity / antisigma factor binding / sporulation resulting in formation of a cellular spore Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Seavers, P.R. / Lewis, R.J. / Brannigan, J.A. / Verschueren, K.H.G. / Murshudov, G.N. / Wilkinson, A.J. | ||||||
![]() | ![]() Title: Structure of the Bacillus Cell Fate Determinant Spoiiaa in Phosphorylated and Unphosphorylated Forms Authors: Seavers, P.R. / Lewis, R.J. / Brannigan, J.A. / Verschueren, K.H.G. / Murshudov, G.N. / Wilkinson, A.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 64.9 KB | Display | ![]() |
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PDB format | ![]() | 52.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 440.3 KB | Display | ![]() |
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Full document | ![]() | 447.7 KB | Display | |
Data in XML | ![]() | 9.6 KB | Display | |
Data in CIF | ![]() | 14.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 13205.202 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | Compound details | IN THE PHOSPHORYL | Sequence details | THE SEQUENCE SPOIIA FROM BACILLUS SPHAERICUSS STRAIN ATCC14577, IS SIMILAR TO THE SWISSPROT ENTRY ...THE SEQUENCE SPOIIA FROM BACILLUS SPHAERICUS | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.96 Å3/Da / Density % sol: 37.35 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 6.5 Details: 30% PEG 5K MME, 200MM (NH4)2SO4, 100MM MES (PH 6.5) | ||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 291 K / pH: 8.5 / Method: vapor diffusion, hanging drop / Details: Seavers, P.R., (2001) Acta Crystallogr., D57, 292. | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.934 Å / Relative weight: 1 |
Reflection | Resolution: 1.16→20 Å / Num. obs: 72227 / % possible obs: 97.9 % / Redundancy: 3.8 % / Rmerge(I) obs: 0.068 / Net I/σ(I): 18.9 |
Reflection shell | Resolution: 1.16→1.2 Å / Redundancy: 2.5 % / Rmerge(I) obs: 0.3 / Mean I/σ(I) obs: 3.72 / % possible all: 85.4 |
Reflection | *PLUS Num. measured all: 277544 |
Reflection shell | *PLUS % possible obs: 85.4 % / Rmerge(I) obs: 0.3 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL PLUS MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.16→19.61 Å
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Refine LS restraints |
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