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Yorodumi- PDB-1fuw: SOLUTION STRUCTURE AND BACKBONE DYNAMICS OF A DOUBLE MUTANT SINGL... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1fuw | ||||||
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Title | SOLUTION STRUCTURE AND BACKBONE DYNAMICS OF A DOUBLE MUTANT SINGLE-CHAIN MONELLIN(SCM) DETERMINED BY NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY | ||||||
Components | MONELLIN | ||||||
Keywords | PLANT PROTEIN / beta-sheet / alpha-helix / loop | ||||||
Function / homology | Function and homology information Monellin, A chain / Monellin, A chain superfamily / Monellin, B chain / : / Monellin / Monellin / Nuclear Transport Factor 2; Chain: A, - #10 / Cystatin superfamily / Nuclear Transport Factor 2; Chain: A, / Roll / Alpha Beta Similarity search - Domain/homology | ||||||
Biological species | Dioscoreophyllum cumminsii (serendipity berry) | ||||||
Method | SOLUTION NMR | ||||||
Model type details | minimized average | ||||||
Authors | Sung, Y.H. / Shin, J. / Jung, J. / Lee, W. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2001 Title: Solution structure, backbone dynamics, and stability of a double mutant single-chain monellin. structural origin of sweetness. Authors: Sung, Y.H. / Shin, J. / Chang, H.J. / Cho, J.M. / Lee, W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1fuw.cif.gz | 629.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1fuw.ent.gz | 528 KB | Display | PDB format |
PDBx/mmJSON format | 1fuw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1fuw_validation.pdf.gz | 346.4 KB | Display | wwPDB validaton report |
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Full document | 1fuw_full_validation.pdf.gz | 541.9 KB | Display | |
Data in XML | 1fuw_validation.xml.gz | 45.6 KB | Display | |
Data in CIF | 1fuw_validation.cif.gz | 69.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fu/1fuw ftp://data.pdbj.org/pub/pdb/validation_reports/fu/1fuw | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10778.295 Da / Num. of mol.: 1 / Mutation: D7E, R39K Source method: isolated from a genetically manipulated source Details: CHAINS B AND A IN A SINGLE CHAIN Source: (gene. exp.) Dioscoreophyllum cumminsii (serendipity berry) Plasmid: PET21 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P02882, UniProt: P02881 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample conditions | pH: 7 / Pressure: ambient / Temperature: 298 K | |||||||||||||||
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 500 MHz |
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-Processing
NMR representative | Selection criteria: minimized average structure |
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NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 50 / Conformers submitted total number: 21 |