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Yorodumi- PDB-1ece: ACIDOTHERMUS CELLULOLYTICUS ENDOCELLULASE E1 CATALYTIC DOMAIN IN ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1ece | |||||||||
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Title | ACIDOTHERMUS CELLULOLYTICUS ENDOCELLULASE E1 CATALYTIC DOMAIN IN COMPLEX WITH A CELLOTETRAOSE | |||||||||
Components | ENDOCELLULASE E1 | |||||||||
Keywords | GLYCOSYL HYDROLASE / CELLULASE / ENDOCELLULASE | |||||||||
Function / homology | Function and homology information cellulase / cellulase activity / polysaccharide binding / cellulose catabolic process Similarity search - Function | |||||||||
Biological species | Acidothermus cellulolyticus (bacteria) | |||||||||
Method | X-RAY DIFFRACTION / Resolution: 2.4 Å | |||||||||
Authors | Sakon, J. / Thomas, S.R. / Himmel, M.E. / Karplus, P.A. | |||||||||
Citation | Journal: Biochemistry / Year: 1996 Title: Crystal structure of thermostable family 5 endocellulase E1 from Acidothermus cellulolyticus in complex with cellotetraose. Authors: Sakon, J. / Adney, W.S. / Himmel, M.E. / Thomas, S.R. / Karplus, P.A. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ece.cif.gz | 155.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ece.ent.gz | 126.2 KB | Display | PDB format |
PDBx/mmJSON format | 1ece.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1ece_validation.pdf.gz | 1 MB | Display | wwPDB validaton report |
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Full document | 1ece_full_validation.pdf.gz | 1 MB | Display | |
Data in XML | 1ece_validation.xml.gz | 30.6 KB | Display | |
Data in CIF | 1ece_validation.cif.gz | 44.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ec/1ece ftp://data.pdbj.org/pub/pdb/validation_reports/ec/1ece | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 40264.641 Da / Num. of mol.: 2 / Fragment: CATALYTIC DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) Acidothermus cellulolyticus (bacteria) / Gene: PVU I FRAGMENT OF A. / Plasmid: PIJ702 / Species (production host): Streptomyces lividans Gene (production host): PVU I FRAGMENT OF A. CELLULOLYTICUS GENOMIC DNA CARRYING NATIVE E1 GENE Production host: Streptomyces lividans TK24 (bacteria) / Strain (production host): TK24 / References: UniProt: P54583, cellulase #2: Polysaccharide | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 4.37 Å3/Da / Density % sol: 65 % | ||||||||||||||||||||||||||||||
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Crystal | *PLUS Density % sol: 68 % | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 4 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 2.4→42.2 Å / Num. obs: 47386 / % possible obs: 90 % / Observed criterion σ(I): 0 |
Reflection | *PLUS Lowest resolution: 9999 Å / Redundancy: 2.2 % / Rmerge(I) obs: 0.092 |
-Processing
Software |
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Refinement | Resolution: 2.4→10 Å / σ(F): 0
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Displacement parameters | Biso mean: 28.1 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.4→10 Å
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Refine LS restraints |
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Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_improper_angle_deg / Dev ideal: 1.2 |