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Yorodumi- PDB-1cee: SOLUTION STRUCTURE OF CDC42 IN COMPLEX WITH THE GTPASE BINDING DO... -
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-Basic information
Entry | Database: PDB / ID: 1cee | ||||||
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Title | SOLUTION STRUCTURE OF CDC42 IN COMPLEX WITH THE GTPASE BINDING DOMAIN OF WASP | ||||||
Components |
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Keywords | Structural protein Regulation / CDC42 ACTIN REGULATOR GTPASE AND THE GTPASE BINDING DOMAIN OF ITS EFFECTOR WASP | ||||||
Function / homology | Function and homology information regulation of T cell antigen processing and presentation / GBD domain binding / submandibular salivary gland formation / actin filament branching / Golgi transport complex / positive regulation of pinocytosis / modification of synaptic structure / regulation of actin polymerization or depolymerization / endothelin receptor signaling pathway involved in heart process / Cdc42 protein signal transduction ...regulation of T cell antigen processing and presentation / GBD domain binding / submandibular salivary gland formation / actin filament branching / Golgi transport complex / positive regulation of pinocytosis / modification of synaptic structure / regulation of actin polymerization or depolymerization / endothelin receptor signaling pathway involved in heart process / Cdc42 protein signal transduction / cardiac neural crest cell migration involved in outflow tract morphogenesis / positive regulation of synapse structural plasticity / dendritic cell migration / GTPase regulator activity / storage vacuole / actin filament-based movement / apolipoprotein A-I receptor binding / positive regulation of epithelial cell proliferation involved in lung morphogenesis / neuron fate determination / modulation by host of viral process / organelle transport along microtubule / regulation of attachment of spindle microtubules to kinetochore / positive regulation of pseudopodium assembly / Inactivation of CDC42 and RAC1 / cardiac conduction system development / GTP-dependent protein binding / regulation of filopodium assembly / establishment of Golgi localization / leading edge membrane / neuropilin signaling pathway / negative regulation of cell motility / positive regulation of intracellular protein transport / cell junction assembly / filopodium assembly / vesicle membrane / actin polymerization or depolymerization / regulation of modification of postsynaptic structure / establishment of epithelial cell apical/basal polarity / dendritic spine morphogenesis / mitogen-activated protein kinase kinase kinase binding / embryonic heart tube development / thioesterase binding / regulation of stress fiber assembly / RHO GTPases activate KTN1 / regulation of lamellipodium assembly / nuclear migration / DCC mediated attractive signaling / adherens junction organization / sprouting angiogenesis / Wnt signaling pathway, planar cell polarity pathway / CD28 dependent Vav1 pathway / regulation of postsynapse organization / positive regulation of filopodium assembly / negative regulation of stress fiber assembly / regulation of mitotic nuclear division / endosomal transport / establishment or maintenance of cell polarity / RHOV GTPase cycle / phagocytosis, engulfment / positive regulation of double-strand break repair via homologous recombination / heart contraction / Myogenesis / RHOJ GTPase cycle / Golgi organization / RHOQ GTPase cycle / positive regulation of cytokinesis / RHO GTPases activate PAKs / phospholipase binding / CDC42 GTPase cycle / RHOU GTPase cycle / macrophage differentiation / Generation of second messenger molecules / RHOG GTPase cycle / RAC3 GTPase cycle / RAC2 GTPase cycle / RHO GTPases Activate WASPs and WAVEs / positive regulation of DNA replication / RHO GTPases activate IQGAPs / epidermis development / spindle midzone / negative regulation of protein-containing complex assembly / positive regulation of lamellipodium assembly / phagocytic vesicle / positive regulation of substrate adhesion-dependent cell spreading / positive regulation of stress fiber assembly / GPVI-mediated activation cascade / RAC1 GTPase cycle / EPHB-mediated forward signaling / substantia nigra development / actin filament polymerization / Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation / T cell activation / small monomeric GTPase / G protein activity / filopodium / secretory granule / actin filament organization / actin filament / RHO GTPases Activate Formins / integrin-mediated signaling pathway Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / SIMULATED ANNEALING, TORSION ANGLE DYNAMICS | ||||||
Authors | Abdul-Manan, N. / Aghazadeh, B. / Liu, G.A. / Majumdar, A. / Ouerfelli, O. / Rosen, M.K. | ||||||
Citation | Journal: Nature / Year: 1999 Title: Structure of Cdc42 in complex with the GTPase-binding domain of the 'Wiskott-Aldrich syndrome' protein. Authors: Abdul-Manan, N. / Aghazadeh, B. / Liu, G.A. / Majumdar, A. / Ouerfelli, O. / Siminovitch, K.A. / Rosen, M.K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1cee.cif.gz | 1.5 MB | Display | PDBx/mmCIF format |
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PDB format | pdb1cee.ent.gz | 1.2 MB | Display | PDB format |
PDBx/mmJSON format | 1cee.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ce/1cee ftp://data.pdbj.org/pub/pdb/validation_reports/ce/1cee | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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NMR ensembles |
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-Components
#1: Protein | Mass: 19871.820 Da / Num. of mol.: 1 / Fragment: CDC42 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Plasmid: PET11A / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: P60953 |
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#2: Protein | Mass: 6392.066 Da / Num. of mol.: 1 / Fragment: GTPASE BINDING DOMAIN OF WASP Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Plasmid: PET11A / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: P42768 |
#3: Chemical | ChemComp-MG / |
#4: Chemical | ChemComp-GCP / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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NMR details | Text: THE STRUCTURE WAS DETERMINED USING A COMBINATION OF TRIPLE- AND QUADRUPLE- RESONANCE NMR EXPERIMENTS ON 13C/ 15N AND PARTIALLY OR FULLY DEUTERATED SAMPLES WITH SELECTIVE METHYL LABELING AT ...Text: THE STRUCTURE WAS DETERMINED USING A COMBINATION OF TRIPLE- AND QUADRUPLE- RESONANCE NMR EXPERIMENTS ON 13C/ 15N AND PARTIALLY OR FULLY DEUTERATED SAMPLES WITH SELECTIVE METHYL LABELING AT VALINE,LUECINE AND ISOLEUCINES. |
-Sample preparation
Sample conditions | Ionic strength: 50mM / pH: 7.0 / Pressure: 1 atm / Temperature: 298 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Varian UNITY INOVA / Manufacturer: Varian / Model: UNITY INOVA / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: SIMULATED ANNEALING, TORSION ANGLE DYNAMICS / Software ordinal: 1 Details: X-PLOR 3.851 WAS USED IN COMBINATION WITH ARIA (REF. M. NILGES) | ||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: LEAST RESTRAINT VIOLATION / Conformers calculated total number: 80 / Conformers submitted total number: 20 |