+Open data
-Basic information
Entry | Database: PDB / ID: 191d | ||||||||||||||||||
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Title | CRYSTAL STRUCTURE OF INTERCALATED FOUR-STRANDED D(C3T) | ||||||||||||||||||
Components | DNA (5'-D(*Keywords | DNA / U-DNA / QUADRUPLE HELIX / PARALLEL-STRANDED TETRAPLEX / BASE INTERCALATED / MISMATCHED | Function / homology | DNA | Function and homology information Method | X-RAY DIFFRACTION / Resolution: 1.4 Å | Authors | Kang, C. / Berger, I. / Lockshin, C. / Ratliff, R. / Moyzis, R. / Rich, A. | Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 1994 | Title: Crystal structure of intercalated four-stranded d(C3T) at 1.4 angstroms resolution. Authors: Kang, C.H. / Berger, I. / Lockshin, C. / Ratliff, R. / Moyzis, R. / Rich, A. History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 191d.cif.gz | 19.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb191d.ent.gz | 13.1 KB | Display | PDB format |
PDBx/mmJSON format | 191d.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 191d_validation.pdf.gz | 374.5 KB | Display | wwPDB validaton report |
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Full document | 191d_full_validation.pdf.gz | 381.9 KB | Display | |
Data in XML | 191d_validation.xml.gz | 4.8 KB | Display | |
Data in CIF | 191d_validation.cif.gz | 6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/91/191d ftp://data.pdbj.org/pub/pdb/validation_reports/91/191d | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: DNA chain | Mass: 1126.781 Da / Num. of mol.: 4 / Source method: obtained synthetically #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 1.76 Å3/Da / Density % sol: 29.93 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Method: vapor diffusion, hanging drop / pH: 6.5 / Details: pH 6.50, VAPOR DIFFUSION, HANGING DROP | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS Method: vapor diffusion, hanging drop / PH range low: 7 / PH range high: 6 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 277 K |
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Detector | Type: RIGAKU RAXIS IIC / Detector: IMAGE PLATE |
Radiation | Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Highest resolution: 1.4 Å |
-Processing
Software | Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Resolution: 1.4→10 Å / σ(F): 2
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Refine Biso |
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Refinement step | Cycle: LAST / Resolution: 1.4→10 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 1.4 Å / Lowest resolution: 10 Å / σ(F): 2 / Rfactor obs: 0.177 / Rfactor Rfree: 0.225 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_d / Dev ideal: 4 |