[English] 日本語
Yorodumi- PDB-1gtz: Structure of STREPTOMYCES COELICOLOR TYPE II DEHYDROQUINASE R23A ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1gtz | ||||||
---|---|---|---|---|---|---|---|
Title | Structure of STREPTOMYCES COELICOLOR TYPE II DEHYDROQUINASE R23A MUTANT IN COMPLEX WITH DEHYDROSHIKIMATE | ||||||
Components | 3-DEHYDROQUINATE DEHYDRATASE | ||||||
Keywords | LYASE / TYPE II DEHYDROQUINASE / SHIKIMATE PATHWAY / DODECAMERIC QUATERNARY STRUCTURE / TETRAHEDRAL SYMMETRY AROMATIC AMINO ACID BIOSYNTHESIS | ||||||
Function / homology | Function and homology information quinate catabolic process / 3-dehydroquinate dehydratase / 3-dehydroquinate dehydratase activity / chorismate biosynthetic process / aromatic amino acid family biosynthetic process / amino acid biosynthetic process Similarity search - Function | ||||||
Biological species | STREPTOMYCES COELICOLOR (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.6 Å | ||||||
Authors | Roszak, A.W. / Krell, T. / Robinson, D.A. / Hunter, I.S. / Coggins, J.R. / Lapthorn, A.J. | ||||||
Citation | Journal: Structure / Year: 2002 Title: The Structure and Mechanism of the Type II Dehydroquinase from Streptomyces Coelicolor Authors: Roszak, A.W. / Robinson, D.A. / Krell, T. / Hunter, I.S. / Fredrickson, M. / Abell, C. / Coggins, J.R. / Lapthorn, A.J. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1gtz.cif.gz | 721.6 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1gtz.ent.gz | 600.7 KB | Display | PDB format |
PDBx/mmJSON format | 1gtz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gt/1gtz ftp://data.pdbj.org/pub/pdb/validation_reports/gt/1gtz | HTTPS FTP |
---|
-Related structure data
Related structure data | 1d0iC 1gu0C 1gu1C 2dhqS C: citing same article (ref.) S: Starting model for refinement |
---|---|
Similar structure data |
-Links
-Assembly
Deposited unit |
| ||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||||||||||||||||||||||||||||||||||||||||||
Unit cell |
| ||||||||||||||||||||||||||||||||||||||||||||||||
Noncrystallographic symmetry (NCS) | NCS oper:
|
-Components
#1: Protein | Mass: 16483.488 Da / Num. of mol.: 12 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) STREPTOMYCES COELICOLOR (bacteria) / Plasmid: PTB-361 / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21 / References: UniProt: P15474, 3-dehydroquinate dehydratase #2: Chemical | ChemComp-TRS / #3: Chemical | ChemComp-DHK / #4: Water | ChemComp-HOH / | Compound details | FUNCTION: CATALYSIS VIA AN ENOLATE FOR A TRANS-DEHYDRATIO | |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.9 Å3/Da / Density % sol: 56.5 % Description: DATA PROCESSED TO CORNERS OF DETECTOR THEREFORE LOW COMPLETENESS OF HIGHEST RESOLUTION SHELL |
---|---|
Crystal grow | pH: 8.5 Details: PEG 8000, SODIUM/POTASSIUM PHOSPHATE, TRIS BUFFER, pH 8.50 |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: SRS / Beamline: PX9.6 / Wavelength: 0.87 |
Detector | Type: ADC QUANTUM 4 / Detector: CCD / Date: Mar 15, 1998 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.87 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→50 Å / Num. obs: 213906 / % possible obs: 98.7 % / Redundancy: 6 % / Rmerge(I) obs: 0.064 |
Reflection shell | Resolution: 1.6→1.63 Å / % possible all: 21.5 |
-Processing
Software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2DHQ Resolution: 1.6→45 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Details: NONE
| ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.6→45 Å
|