ジャーナル: J Cell Biol / 年: 2000 タイトル: Visualization of tRNA movements on the Escherichia coli 70S ribosome during the elongation cycle. 著者: R K Agrawal / C M Spahn / P Penczek / R A Grassucci / K H Nierhaus / J Frank / 要旨: Three-dimensional cryomaps have been reconstructed for tRNA-ribosome complexes in pre- and posttranslocational states at 17-A resolution. The positions of tRNAs in the A and P sites in the ...Three-dimensional cryomaps have been reconstructed for tRNA-ribosome complexes in pre- and posttranslocational states at 17-A resolution. The positions of tRNAs in the A and P sites in the pretranslocational complexes and in the P and E sites in the posttranslocational complexes have been determined. Of these, the P-site tRNA position is the same as seen earlier in the initiation-like fMet-tRNA(f)(Met)-ribosome complex, where it was visualized with high accuracy. Now, the positions of the A- and E-site tRNAs are determined with similar accuracy. The positions of the CCA end of the tRNAs at the A site are different before and after peptide bond formation. The relative positions of anticodons of P- and E-site tRNAs in the posttranslocational state are such that a codon-anticodon interaction at the E site appears feasible.
履歴
登録
2000年7月19日
登録サイト: RCSB / 処理サイト: RCSB
改定 1.0
2000年8月11日
Provider: repository / タイプ: Initial release
改定 1.1
2008年4月27日
Group: Version format compliance
改定 1.2
2011年7月13日
Group: Non-polymer description / Version format compliance
モード: BRIGHT FIELD / 倍率(公称値): 52200 X / 最大 デフォーカス(公称値): 2200 nm / 最小 デフォーカス(公称値): 1200 nm
試料ホルダ
試料ホルダーモデル: GATAN 626 SINGLE TILT LIQUID NITROGEN CRYO TRANSFER HOLDER
撮影
電子線照射量: 10 e/Å2 / フィルム・検出器のモデル: GENERIC FILM
画像スキャン
Scanner model: PERKIN ELMER
-
解析
対称性
点対称性: C1 (非対称)
3次元再構成
解像度: 17 Å / 解像度の算出法: FSC 3 SIGMA CUT-OFF / 粒子像の数: 15610 / 対称性のタイプ: POINT
原子モデル構築
空間: REAL 詳細: DETAILS--TRNA COORDINATES (TRNA09) WERE FIT INTO CRYO-EM MAP AS RIGID BODIES THE RELATIVE POSITIONS OF THE TRNA IS DERIVED BY FITTING THE X-RAY STRUCTURE OF TRNA (TRNA09) INTO THE THREE- ...詳細: DETAILS--TRNA COORDINATES (TRNA09) WERE FIT INTO CRYO-EM MAP AS RIGID BODIES THE RELATIVE POSITIONS OF THE TRNA IS DERIVED BY FITTING THE X-RAY STRUCTURE OF TRNA (TRNA09) INTO THE THREE-DIMENSIONAL CRYO-ELECTRON MICROSCOPY MAPS OF FUNCTIONAL COMPLEXES OF THE E. COLI 70S RIBOSOMES. IT SHOULD BE NOTED THAT ALL TRNA-BINDING POSITIONS ARE NOT OCCUPIED SIMULTANEOSLY. THEREFORE, THERE ARE OVERLAPS BETWEEN THE TWO TRNA COORDINATES. IN ORDER TO SEE THE RELATIVE POSITIONS OF VARIOUS RIBOSOMAL DOMAINS, CHAIN C (THE P-SITE TRNA) SHOULD BE ALIGNED WITH OUR EARLIER DEPOSITED P-SITE POSITION (ID CODE 1EGO).