rRNA cytidine N-acetyltransferase activity / tRNA acetylation / mRNA modification / Mpp10 complex / rRNA (pseudouridine) methyltransferase activity / rRNA modification / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / nuclear microtubule / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / box C/D RNP complex ...rRNA cytidine N-acetyltransferase activity / tRNA acetylation / mRNA modification / Mpp10 complex / rRNA (pseudouridine) methyltransferase activity / rRNA modification / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / nuclear microtubule / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / box C/D RNP complex / snRNA binding / rRNA base methylation / protein localization to nucleolus / U3 snoRNA binding / rRNA export from nucleus / sno(s)RNA-containing ribonucleoprotein complex / 90S preribosome / preribosome, small subunit precursor / snoRNA binding / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / 転写後修飾 / small-subunit processome / ribosomal small subunit biogenesis / nuclear periphery / 転移酵素; アシル基を移すもの; アミノアシル基以外のアシル基を移すもの / 助触媒 / small ribosomal subunit rRNA binding / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rRNA processing / 転写後修飾 / リボソーム生合成 / methyltransferase activity / cytosolic small ribosomal subunit / ribonucleoprotein complex / small ribosomal subunit / tRNA binding / メチル化 / rRNA binding / リボソーム / structural constituent of ribosome / protein ubiquitination / 翻訳 (生物学) / GTPase activity / GTP binding / 核小体 / RNA binding / 生体膜 / ATP binding / identical protein binding / 細胞核 / 細胞質
Nrap protein domain 6 / RNA 3'-terminal phosphate cyclase domain superfamily / Nrap protein, domain 2 / Nrap protein, domain 3 / Nrap protein, domain 4 / Nrap protein, domain 5 / Nrap protein, domain 6 / Ribosomal protein S8 superfamily / Nop domain superfamily / WD40-repeat-containing domain superfamily ...Nrap protein domain 6 / RNA 3'-terminal phosphate cyclase domain superfamily / Nrap protein, domain 2 / Nrap protein, domain 3 / Nrap protein, domain 4 / Nrap protein, domain 5 / Nrap protein, domain 6 / Ribosomal protein S8 superfamily / Nop domain superfamily / WD40-repeat-containing domain superfamily / Winged helix DNA-binding domain superfamily / RNA 3'-terminal phosphate cyclase, insert domain superfamily / K Homology domain, type 1 superfamily / Ribosomal protein S7 domain superfamily / Ribosomal protein S11 superfamily / RNA-binding S4 domain superfamily / Ribosome biogenesis protein Bms1, N-terminal / RNA cytidine acetyltransferase NAT10 / WD repeat-containing protein WDR46/Utp7 / Nop, C-terminal domain / Ribosomal protein S4, KOW domain / Krr1, KH1 domain / Rrp7, RRM-like N-terminal domain / Ribosomal RNA-processing protein 7 / Nucleolar protein 10/Enp2 / U3 small nucleolar RNA-associated protein 10 / Ribosomal protein S19e domain superfamily / Pre-rRNA-processing protein PNO1-like / Fcf2/DNTTIP2 / Ribosome biogenesis protein Bms1/Tsr1 / Ribosomal RNA-processing protein Rrp9-like / Protein AATF/Bfr2 / Ribosomal protein S4e, central domain superfamily / Nrap protein domain 1 / TmcA/NAT10/Kre33 / Fibrillarin / Ribosomal RNA assembly KRR1 / U3 small nucleolar RNA-associated protein 15, C-terminal / WD40 repeat, conserved site / Ribosomal protein S17, conserved site / G-protein beta WD-40 repeat / Ribosomal protein S5 domain 2-type fold / Ribosomal protein S9, conserved site / Ribosomal protein S7, conserved site / Fibrillarin, conserved site / U3 small nucleolar RNA-associated protein 10, N-terminal / Ribosomal protein S8e/ribosomal biogenesis NSA2 / Ribosomal protein S4/S9 / Ribosomal protein S15P / RNA 3'-terminal phosphate cyclase domain / Ribosomal protein S7 domain / Ribosomal RNA-processing protein 7, C-terminal domain / 40S ribosomal protein S11, N-terminal / Ribosomal protein S28e conserved site / 40S ribosomal protein S4, C-terminal domain / Bms1/Tsr1-type G domain / 50S ribosomal protein L30e-like / Alpha/beta knot methyltransferases / tRNA (guanine-N1-)-methyltransferase, N-terminal / Helix hairpin bin domain superfamily / Ribosomal protein S17, archaeal/eukaryotic / AATF leucine zipper-containing domain / Possible tRNA binding domain / 40S ribosomal protein S1/3, eukaryotes / 30s ribosomal protein S13, C-terminal / P-loop containing nucleoside triphosphate hydrolase / Nucleolar complex protein 4 / Periodic tryptophan protein 2 / Nucleotidyltransferase superfamily / snoRNA binding domain, fibrillarin / Ribosomal protein L7Ae/L8/Nhp2 family / U3 small nucleolar RNA-associated protein 6 / Ribosomal protein S9/S16 / Ribosomal protein S17 / Ribosomal_S17 N-terminal / Ribosomal protein S13/S18 / Ribosomal protein S8 / Ribosomal protein S12/S23 / Ribosomal protein S24e / Ribosomal protein S28e / Ribosomal protein S27 / Ribosomal protein S19e / Dip2/Utp12 Family / Nop14-like family / Sas10/Utp3/C1D family / Sas10 C-terminal domain / BING4CT (NUC141) domain / Ribosomal S13/S15 N-terminal domain / Down-regulated in metastasis / Nrap protein PAP/OAS1-like domain 5 / Nrap protein nucleotidyltransferase domain 4 / Nrap protein domain 3 / Nrap protein PAP/OAS-like domain / Nrap protein domain 1 / Utp21 specific WD40 associated putative domain / CBF/Mak21 family / BP28CT (NUC211) domain / U3 snoRNA associated / UTP15 C terminal / Utp14 protein
WD_REPEATS_REGION domain-containing protein / 40S ribosomal protein S4 / RNA cytidine acetyltransferase / 40S ribosomal protein s23-like protein / 40S ribosomal protein s5-like protein / 40S ribosomal protein S6 / Ribosomal protein s27-like protein / 40S ribosomal protein S8 / RNA 3'-terminal phosphate cyclase-like protein / 40S ribosomal protein s9-like protein ...WD_REPEATS_REGION domain-containing protein / 40S ribosomal protein S4 / RNA cytidine acetyltransferase / 40S ribosomal protein s23-like protein / 40S ribosomal protein s5-like protein / 40S ribosomal protein S6 / Ribosomal protein s27-like protein / 40S ribosomal protein S8 / RNA 3'-terminal phosphate cyclase-like protein / 40S ribosomal protein s9-like protein / Utp12 domain-containing protein / 40S ribosomal protein S13-like protein / DDB1- and CUL4-associated factor 13 / WD_REPEATS_REGION domain-containing protein / CBF domain-containing protein / Nucleolar protein 58 / U3 small nucleolar RNA-associated protein 22 / Uncharacterized protein / Nucleolar protein 58 / Histone-glutamine methyltransferase / WD_REPEATS_REGION domain-containing protein / Uncharacterized protein / S4 RNA-binding domain-containing protein / 40S ribosomal protein S24 / Uncharacterized protein CTHT_0071780 / DRIM domain-containing protein / 40S ribosomal protein S22-like protein / Ribonucloprotein / 40S ribosomal protein S16-like protein / WD_REPEATS_REGION domain-containing protein / Uncharacterized protein / 40S ribosomal protein S14-like protein / 40S ribosomal protein S19-like protein / Periodic tryptophan protein 2-like protein / U3 small nucleolar RNA-associated protein 11 / 40S ribosomal protein S11-like protein / WD_REPEATS_REGION domain-containing protein / WD_REPEATS_REGION domain-containing protein / WD_REPEATS_REGION domain-containing protein / Putative U3 small nucleolar ribonucleoprotein / U3 small nucleolar RNA-associated protein 10 / Uncharacterized protein / Pre-rRNA-processing protein PNO1 / KRR1 small subunit processome component / Bms1-type G domain-containing protein / Fcf2 domain-containing protein / Sas10 domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Putative ribosomal protein / 40S ribosomal protein S1 / 40S ribosomal protein S7 / Uncharacterized protein / Ribosome biogenesis protein ENP2 / Putative U3 small nucleolar ribonucleoprotein protein / 40S ribosomal protein S28-like protein / Protein BFR2 / Putative U3 snoRNP protein
ジャーナル: Mol Cell / 年: 2019 タイトル: Thermophile 90S Pre-ribosome Structures Reveal the Reverse Order of Co-transcriptional 18S rRNA Subdomain Integration. 著者: Jingdong Cheng / Jochen Baßler / Paulina Fischer / Benjamin Lau / Nikola Kellner / Ruth Kunze / Sabine Griesel / Martina Kallas / Otto Berninghausen / Daniela Strauss / Roland Beckmann / Ed Hurt / 要旨: Eukaryotic ribosome biogenesis involves RNA folding and processing that depend on assembly factors and small nucleolar RNAs (snoRNAs). The 90S (SSU-processome) is the earliest pre-ribosome ...Eukaryotic ribosome biogenesis involves RNA folding and processing that depend on assembly factors and small nucleolar RNAs (snoRNAs). The 90S (SSU-processome) is the earliest pre-ribosome structurally analyzed, which was suggested to assemble stepwise along the growing pre-rRNA from 5' > 3', but this directionality may not be accurate. Here, by analyzing the structure of a series of 90S assembly intermediates from Chaetomium thermophilum, we discover a reverse order of 18S rRNA subdomain incorporation. Large parts of the 18S rRNA 3' and central domains assemble first into the 90S before the 5' domain is integrated. This final incorporation depends on a contact between a heterotrimer Enp2-Bfr2-Lcp5 recruited to the flexible 5' domain and Kre33, which reconstitutes the Kre33-Enp-Brf2-Lcp5 module on the compacted 90S. Keeping the 5' domain temporarily segregated from the 90S scaffold could provide extra time to complete the multifaceted 5' domain folding, which depends on a distinct set of snoRNAs and processing factors.