+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 2qiu | ||||||
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タイトル | Structure of Human Arg-Insulin | ||||||
要素 | (Insulin) x 2 | ||||||
キーワード | HORMONE / Glucose Utilisation / T3R3 conformation | ||||||
機能・相同性 | 機能・相同性情報 negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / positive regulation of nitric oxide mediated signal transduction / negative regulation of fatty acid metabolic process / negative regulation of feeding behavior / Signaling by Insulin receptor / IRS activation / Insulin processing / regulation of protein secretion / positive regulation of peptide hormone secretion ...negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / positive regulation of nitric oxide mediated signal transduction / negative regulation of fatty acid metabolic process / negative regulation of feeding behavior / Signaling by Insulin receptor / IRS activation / Insulin processing / regulation of protein secretion / positive regulation of peptide hormone secretion / positive regulation of respiratory burst / negative regulation of acute inflammatory response / Regulation of gene expression in beta cells / alpha-beta T cell activation / regulation of cellular amino acid metabolic process / negative regulation of respiratory burst involved in inflammatory response / positive regulation of dendritic spine maintenance / positive regulation of glycogen biosynthetic process / Synthesis, secretion, and deacylation of Ghrelin / negative regulation of protein secretion / regulation of protein localization to plasma membrane / fatty acid homeostasis / Signal attenuation / negative regulation of lipid catabolic process / negative regulation of gluconeogenesis / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / COPI-mediated anterograde transport / positive regulation of lipid biosynthetic process / negative regulation of reactive oxygen species biosynthetic process / negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / positive regulation of insulin receptor signaling pathway / nitric oxide-cGMP-mediated signaling / transport vesicle / positive regulation of protein autophosphorylation / Insulin receptor recycling / insulin-like growth factor receptor binding / neuron projection maintenance / NPAS4 regulates expression of target genes / positive regulation of protein metabolic process / positive regulation of brown fat cell differentiation / activation of protein kinase B activity / endoplasmic reticulum-Golgi intermediate compartment membrane / positive regulation of glycolytic process / positive regulation of mitotic nuclear division / Insulin receptor signalling cascade / positive regulation of nitric-oxide synthase activity / positive regulation of cytokine production / positive regulation of long-term synaptic potentiation / acute-phase response / Regulation of insulin secretion / negative regulation of protein catabolic process / endosome lumen / positive regulation of glucose import / positive regulation of protein secretion / negative regulation of proteolysis / positive regulation of cell differentiation / regulation of transmembrane transporter activity / insulin receptor binding / wound healing / regulation of synaptic plasticity / vasodilation / hormone activity / cognition / positive regulation of neuron projection development / positive regulation of protein localization to nucleus / Golgi lumen / glucose metabolic process / regulation of protein localization / glucose homeostasis / insulin receptor signaling pathway / cell-cell signaling / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / positive regulation of NF-kappaB transcription factor activity / positive regulation of cell growth / protease binding / secretory granule lumen / positive regulation of MAPK cascade / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of cell migration / G protein-coupled receptor signaling pathway / Amyloid fiber formation / Golgi membrane / endoplasmic reticulum lumen / negative regulation of gene expression / positive regulation of cell population proliferation / positive regulation of gene expression / regulation of DNA-templated transcription / extracellular space / extracellular region / identical protein binding 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | X線回折 / 分子置換 / 解像度: 2 Å | ||||||
データ登録者 | Sreekanth, R. / Pattabhi, V. / Rajan, S.S. | ||||||
引用 | ジャーナル: Biochimie / 年: 2008 タイトル: Structural interpretation of reduced insulin activity as seen in the crystal structure of human Arg-insulin 著者: Sreekanth, R. / Pattabhi, V. / Rajan, S.S. #1: ジャーナル: Nature / 年: 1976 タイトル: Structure of insulin in 4-zinc insulin 著者: Bentley, G. / Dodson, E. / Dodson, G. / Hodgkin, D. / Mercola, D. | ||||||
履歴 |
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Remark 650 | HELIX Determination method: Author determined |
-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 2qiu.cif.gz | 33.8 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb2qiu.ent.gz | 23.5 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 2qiu.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 2qiu_validation.pdf.gz | 456.5 KB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 2qiu_full_validation.pdf.gz | 461 KB | 表示 | |
XML形式データ | 2qiu_validation.xml.gz | 7.8 KB | 表示 | |
CIF形式データ | 2qiu_validation.cif.gz | 9.8 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/qi/2qiu ftp://data.pdbj.org/pub/pdb/validation_reports/qi/2qiu | HTTPS FTP |
-関連構造データ
関連構造データ | 1zniS S: 精密化の開始モデル |
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類似構造データ |
-リンク
-集合体
登録構造単位 |
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単位格子 |
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Components on special symmetry positions |
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-要素
#1: タンパク質・ペプチド | 分子量: 2540.892 Da / 分子数: 2 / 断片: Insulin A chain / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 参照: UniProt: P01308 #2: タンパク質・ペプチド | 分子量: 3433.953 Da / 分子数: 2 / 断片: Insulin B chain / 由来タイプ: 天然 / 由来: (天然) Homo sapiens (ヒト) / 参照: UniProt: P01308 #3: 化合物 | #4: 水 | ChemComp-HOH / | |
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-実験情報
-実験
実験 | 手法: X線回折 / 使用した結晶の数: 1 |
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-試料調製
結晶 | マシュー密度: 1.96 Å3/Da / 溶媒含有率: 37.35 % |
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結晶化 | 温度: 293 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 6.7 詳細: Sodium Citrate, Acetone, Zinc Sulphate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-データ収集
回折 | 平均測定温度: 298 K |
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放射光源 | 由来: 回転陽極 / タイプ: RIGAKU RU300 / 波長: 1.5418 Å |
検出器 | タイプ: MARRESEARCH / 検出器: IMAGE PLATE / 日付: 2006年8月31日 |
放射 | モノクロメーター: NIL / プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1.5418 Å / 相対比: 1 |
反射 | 解像度: 2→40.26 Å / Num. all: 6141 / Num. obs: 6141 / % possible obs: 100 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / 冗長度: 5 % / Rmerge(I) obs: 0.12 / Net I/σ(I): 3.4 |
反射 シェル | 解像度: 2→2.07 Å / 冗長度: 4.86 % / Rmerge(I) obs: 0.44 / Mean I/σ(I) obs: 1.2 / % possible all: 100 |
-解析
ソフトウェア |
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精密化 | 構造決定の手法: 分子置換 開始モデル: 1ZNI 解像度: 2→40.26 Å / Cor.coef. Fo:Fc: 0.961 / Cor.coef. Fo:Fc free: 0.93 / SU B: 9.949 / SU ML: 0.254 / 交差検証法: THROUGHOUT / ESU R: 0.313 / ESU R Free: 0.22 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD
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溶媒の処理 | イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso mean: 41.193 Å2
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精密化ステップ | サイクル: LAST / 解像度: 2→40.26 Å
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拘束条件 |
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LS精密化 シェル | 解像度: 2.001→2.053 Å / Total num. of bins used: 20
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