+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 2l4h | ||||||
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タイトル | The Solution Structure of Calcium Bound CIB1 | ||||||
要素 | Calcium and integrin-binding protein 1 | ||||||
キーワード | METAL BINDING PROTEIN / Calcium and Integrin Binding protein 1 | ||||||
機能・相同性 | 機能・相同性情報 calcium-dependent protein kinase inhibitor activity / thrombopoietin-mediated signaling pathway / endomitotic cell cycle / positive regulation of male germ cell proliferation / filopodium tip / positive regulation of calcineurin-NFAT signaling cascade / negative regulation of microtubule depolymerization / protein serine/threonine kinase inhibitor activity / positive regulation of cell adhesion mediated by integrin / positive regulation of cell-matrix adhesion ...calcium-dependent protein kinase inhibitor activity / thrombopoietin-mediated signaling pathway / endomitotic cell cycle / positive regulation of male germ cell proliferation / filopodium tip / positive regulation of calcineurin-NFAT signaling cascade / negative regulation of microtubule depolymerization / protein serine/threonine kinase inhibitor activity / positive regulation of cell adhesion mediated by integrin / positive regulation of cell-matrix adhesion / platelet formation / positive regulation of cell migration involved in sprouting angiogenesis / positive regulation of catalytic activity / regulation of cell division / spermatid development / positive regulation of protein targeting to membrane / negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / negative regulation of megakaryocyte differentiation / cytoplasmic microtubule organization / protein-membrane adaptor activity / positive regulation of substrate adhesion-dependent cell spreading / extrinsic apoptotic signaling pathway / cellular response to nerve growth factor stimulus / negative regulation of protein phosphorylation / response to ischemia / cell periphery / positive regulation of protein localization to plasma membrane / 筋鞘 / positive regulation of protein serine/threonine kinase activity / cellular response to growth factor stimulus / small GTPase binding / ruffle membrane / double-strand break repair / negative regulation of neuron projection development / lamellipodium / cellular response to tumor necrosis factor / positive regulation of NF-kappaB transcription factor activity / regulation of cell population proliferation / 成長円錐 / perikaryon / positive regulation of cell growth / 血管新生 / vesicle / transmembrane transporter binding / positive regulation of ERK1 and ERK2 cascade / 細胞接着 / neuron projection / positive regulation of cell migration / positive regulation of protein phosphorylation / apical plasma membrane / 神経繊維 / 細胞分裂 / negative regulation of cell population proliferation / 中心体 / neuronal cell body / apoptotic process / DNA damage response / calcium ion binding / positive regulation of cell population proliferation / negative regulation of apoptotic process / perinuclear region of cytoplasm / ゴルジ体 / magnesium ion binding / 小胞体 / extracellular exosome / 核質 / 生体膜 / 細胞核 / 細胞膜 / 細胞質 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | 溶液NMR / simulated annealing | ||||||
データ登録者 | Huang, H. / Vogel, H.J. | ||||||
引用 | ジャーナル: J.Biol.Chem. / 年: 2011 タイトル: Solution Structures of Ca2+-CIB1 and Mg2+-CIB1 and Their Interactions with the Platelet Integrin {alpha}IIb Cytoplasmic Domain. 著者: Huang, H. / Ishida, H. / Yamniuk, A.P. / Vogel, H.J. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 2l4h.cif.gz | 635.3 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb2l4h.ent.gz | 526 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 2l4h.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/l4/2l4h ftp://data.pdbj.org/pub/pdb/validation_reports/l4/2l4h | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質 | 分子量: 24507.264 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: CIB1, CIB, KIP, PRKDCIP / プラスミド: pET19b / 発現宿主: Escherichia coli (大腸菌) / 株 (発現宿主): BL21 / 参照: UniProt: Q99828 |
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#2: 化合物 |
-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||
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NMR実験 |
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-試料調製
詳細 |
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試料 |
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試料状態 | イオン強度: 200 / pH: 7.5 / 圧: ambient / 温度: 310 K |
-NMR測定
NMRスペクトロメーター | タイプ: Bruker Avance / 製造業者: Bruker / モデル: AVANCE / 磁場強度: 700 MHz |
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-解析
NMR software | 名称: X-PLOR NIH / バージョン: 2.18 / 開発者: Schwieters, Kuszewski, Tjandra and Clore / 分類: 精密化 |
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精密化 | 手法: simulated annealing / ソフトェア番号: 1 / 詳細: two steps: 1st step, 200K-20K; 2nd step, 20K-2K |
NMR constraints | NOE constraints total: 1069 / NOE intraresidue total count: 80 / NOE long range total count: 118 / NOE medium range total count: 71 / NOE sequential total count: 167 / Protein chi angle constraints total count: 0 / Protein other angle constraints total count: 0 / Protein phi angle constraints total count: 174 / Protein psi angle constraints total count: 174 |
代表構造 | 選択基準: lowest energy |
NMRアンサンブル | Average torsion angle constraint violation: 2.38 ° コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 11 / Maximum lower distance constraint violation: 1.8 Å / Maximum torsion angle constraint violation: 30 ° / Maximum upper distance constraint violation: 8 Å / Torsion angle constraint violation method: TALOS |
NMR ensemble rms | Distance rms dev: 0.086 Å / Distance rms dev error: 0.005 Å |