+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-13111 | ||||||||||||||||||
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タイトル | Cryo-EM structure of the 1 hpf zebrafish embryo 80S ribosome | ||||||||||||||||||
マップデータ | |||||||||||||||||||
試料 |
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機能・相同性 | 機能・相同性情報 Hypusine synthesis from eIF5A-lysine / Platelet degranulation / optokinetic behavior / L13a-mediated translational silencing of Ceruloplasmin expression / SRP-dependent cotranslational protein targeting to membrane / RMTs methylate histone arginines / TNFR1-mediated ceramide production / : / : / Formation of a pool of free 40S subunits ...Hypusine synthesis from eIF5A-lysine / Platelet degranulation / optokinetic behavior / L13a-mediated translational silencing of Ceruloplasmin expression / SRP-dependent cotranslational protein targeting to membrane / RMTs methylate histone arginines / TNFR1-mediated ceramide production / : / : / Formation of a pool of free 40S subunits / Formation of the ternary complex, and subsequently, the 43S complex / Ribosomal scanning and start codon recognition / : / Protein methylation / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / mTORC1-mediated signalling / brain segmentation / TNFR1-induced NF-kappa-B signaling pathway / Regulation of TNFR1 signaling / convergent extension involved in gastrulation / : / Neutrophil degranulation / primitive hemopoiesis / embryonic retina morphogenesis in camera-type eye / death domain binding / positive regulation of translational termination / mitochondrial cytochrome c oxidase assembly / ribosome hibernation / hemoglobin biosynthetic process / : / positive regulation of translational elongation / chordate embryonic development / embryonic brain development / ribosomal subunit / positive regulation of gastrulation / exocrine pancreas development / anatomical structure development / laminin receptor activity / mitochondrial large ribosomal subunit / positive regulation of translational fidelity / definitive hemopoiesis / 膵臓 / regulation of establishment of cell polarity / cytoplasmic side of rough endoplasmic reticulum membrane / erythrocyte development / translational elongation / negative regulation of Wnt signaling pathway / regulation of innate immune response / regulation of cell division / erythrocyte maturation / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / mitochondrial respiratory chain complex I assembly / protein-RNA complex assembly / positive regulation of translational initiation / 造血 / translation regulator activity / intrinsic apoptotic signaling pathway by p53 class mediator / hematopoietic stem cell differentiation / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / 小胞体 / mRNA transport / 脈管形成 / laminin binding / translation elongation factor activity / rescue of stalled ribosome / translation initiation factor binding / 核膜孔 / maturation of SSU-rRNA / translation repressor activity / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / positive regulation of apoptotic signaling pathway / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / ribosomal large subunit biogenesis / cellular response to amino acid starvation / translation initiation factor activity / DNA-(apurinic or apyrimidinic site) lyase / small-subunit processome / negative regulation of autophagy / positive regulation of RNA splicing / 赤血球形成 / protein kinase C binding / apoptotic signaling pathway / brain development / modification-dependent protein catabolic process / mRNA 5'-UTR binding / regulation of erythrocyte differentiation / ribosomal small subunit biogenesis / ribosomal large subunit assembly / spindle / small ribosomal subunit rRNA binding / protein tag activity / ribosomal small subunit assembly / rRNA processing / cytosolic small ribosomal subunit / regulation of protein localization / large ribosomal subunit rRNA binding / cellular response to xenobiotic stimulus 類似検索 - 分子機能 | ||||||||||||||||||
生物種 | Danio rerio (ゼブラフィッシュ) / Zebrafish, Brachydanio rerio | ||||||||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.2 Å | ||||||||||||||||||
データ登録者 | Leesch F / Lorenzo-Orts L / Grishkovskaya I / Kandolf S / Belacic K / Meinhart A / Haselbach D / Pauli A | ||||||||||||||||||
資金援助 | オーストリア, スイス, 5件
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引用 | ジャーナル: Nature / 年: 2023 タイトル: A molecular network of conserved factors keeps ribosomes dormant in the egg. 著者: Friederike Leesch / Laura Lorenzo-Orts / Carina Pribitzer / Irina Grishkovskaya / Josef Roehsner / Anastasia Chugunova / Manuel Matzinger / Elisabeth Roitinger / Katarina Belačić / Susanne ...著者: Friederike Leesch / Laura Lorenzo-Orts / Carina Pribitzer / Irina Grishkovskaya / Josef Roehsner / Anastasia Chugunova / Manuel Matzinger / Elisabeth Roitinger / Katarina Belačić / Susanne Kandolf / Tzi-Yang Lin / Karl Mechtler / Anton Meinhart / David Haselbach / Andrea Pauli / 要旨: Ribosomes are produced in large quantities during oogenesis and are stored in the egg. However, the egg and early embryo are translationally repressed. Here, using mass spectrometry and cryo-electron ...Ribosomes are produced in large quantities during oogenesis and are stored in the egg. However, the egg and early embryo are translationally repressed. Here, using mass spectrometry and cryo-electron microscopy analyses of ribosomes isolated from zebrafish (Danio rerio) and Xenopus laevis eggs and embryos, we provide molecular evidence that ribosomes transition from a dormant state to an active state during the first hours of embryogenesis. Dormant ribosomes are associated with four conserved factors that form two modules, consisting of Habp4-eEF2 and death associated protein 1b (Dap1b) or Dap in complex with eIF5a. Both modules occupy functionally important sites and act together to stabilize ribosomes and repress translation. Dap1b (also known as Dapl1 in mammals) is a newly discovered translational inhibitor that stably inserts into the polypeptide exit tunnel. Addition of recombinant zebrafish Dap1b protein is sufficient to block translation and reconstitute the dormant egg ribosome state in a mammalian translation extract in vitro. Thus, a developmentally programmed, conserved ribosome state has a key role in ribosome storage and translational repression in the egg. | ||||||||||||||||||
履歴 |
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-構造の表示
添付画像 |
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-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_13111.map.gz | 364.2 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-13111-v30.xml emd-13111.xml | 96.9 KB 96.9 KB | 表示 表示 | EMDBヘッダ |
画像 | emd_13111.png | 157 KB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-13111 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-13111 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_13111.map.gz / 形式: CCP4 / 大きさ: 421.9 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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ボクセルのサイズ | X=Y=Z: 1.06 Å | ||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-試料の構成要素
+全体 : 80S ribosome from 1 hpf zebrafish embryos
+超分子 #1: 80S ribosome from 1 hpf zebrafish embryos
+分子 #1: 18S rRNA
+分子 #76: 28S rRNA
+分子 #77: 5S rRNA
+分子 #78: 5.8S rRNA
+分子 #2: 40S ribosomal protein SA
+分子 #3: 40S ribosomal protein S3a
+分子 #4: 40S ribosomal protein S2
+分子 #5: 40S ribosomal protein S4, X isoform
+分子 #6: 40S ribosomal protein S6
+分子 #7: 40S ribosomal protein S7
+分子 #8: 40S ribosomal protein S8
+分子 #9: 40S ribosomal protein S9
+分子 #10: 40S ribosomal protein S11
+分子 #11: 40S ribosomal protein S13
+分子 #12: Ribosomal protein S14
+分子 #13: 40S ribosomal protein S17
+分子 #14: 40S ribosomal protein S21
+分子 #15: 40S ribosomal protein S15a
+分子 #16: 40S ribosomal protein S23
+分子 #17: 40S ribosomal protein S24
+分子 #18: 40S ribosomal protein S26
+分子 #19: 40S ribosomal protein S27
+分子 #20: 40S ribosomal protein S30
+分子 #21: DNA-(apurinic or apyrimidinic site) lyase
+分子 #22: Ribosomal protein S5
+分子 #23: Ribosomal protein S10
+分子 #24: 40S ribosomal protein S15
+分子 #25: Ribosomal protein S16
+分子 #26: 40S ribosomal protein S20
+分子 #27: 40S ribosomal protein S28
+分子 #28: 40S ribosomal protein S29
+分子 #29: Guanine nucleotide-binding protein subunit beta-2-like 1
+分子 #30: 40S ribosomal protein S25
+分子 #31: 40S ribosomal protein S19
+分子 #32: 40S ribosomal protein S18
+分子 #33: Zgc:103482
+分子 #34: 60S ribosomal protein L27
+分子 #35: 60S ribosomal protein L29
+分子 #36: 60S ribosomal protein L30
+分子 #37: 60S ribosomal protein L31
+分子 #38: Ribosomal protein L32
+分子 #39: 60S ribosomal protein L35a
+分子 #40: 60S ribosomal protein L34
+分子 #41: 60S ribosomal protein L35
+分子 #42: 60S ribosomal protein L36
+分子 #43: Ribosomal protein L37
+分子 #44: 60S ribosomal protein L38
+分子 #45: 60S ribosomal protein L36a
+分子 #46: Zgc:171772
+分子 #47: 60S ribosomal protein L8
+分子 #48: Ribosomal protein L3
+分子 #49: Ribosomal protein L4
+分子 #50: Ribosomal protein L5b
+分子 #51: 60S ribosomal protein L6
+分子 #52: Ribosomal protein L7
+分子 #53: 60S ribosomal protein L7a
+分子 #54: 60S ribosomal protein L9
+分子 #55: 60S ribosomal protein L11
+分子 #56: 60S ribosomal protein L13
+分子 #57: 60S ribosomal protein L14
+分子 #58: Ribosomal protein L15
+分子 #59: 60S ribosomal protein L13a
+分子 #60: 60S ribosomal protein L17
+分子 #61: Ribosomal protein L18
+分子 #62: 60S ribosomal protein L19
+分子 #63: 60S ribosomal protein L21
+分子 #64: Ribosomal protein L22
+分子 #65: 60S ribosomal protein L23
+分子 #66: 60S ribosomal protein L24
+分子 #67: Ribosomal protein L23a
+分子 #68: ATPase H+ transporting V0 subunit e1
+分子 #69: Ribosomal protein L39
+分子 #70: 60S ribosomal protein L41
+分子 #71: 60S ribosomal protein L28
+分子 #72: 60S ribosomal protein L10
+分子 #73: 60S ribosomal protein L18a
+分子 #74: 60S ribosomal protein L40
+分子 #75: 60S ribosomal protein L27a
+分子 #79: Novel protein similar to human proliferation-associated 2G4 prote...
+分子 #80: Eukaryotic translation elongation factor 2
+分子 #81: Eukaryotic translation initiation factor 5A
+分子 #82: Dap1b
+分子 #83: ZINC ION
+分子 #84: MAGNESIUM ION
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 7.6 構成要素:
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グリッド | モデル: Quantifoil R3.5/1 / 材質: COPPER / メッシュ: 200 / 支持フィルム - 材質: CARBON / 支持フィルム - トポロジー: CONTINUOUS / 支持フィルム - Film thickness: 2.0 nm / 前処理 - タイプ: GLOW DISCHARGE / 前処理 - 時間: 60 sec. / 前処理 - 雰囲気: AIR | ||||||||||||||||||
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 70 % / 装置: LEICA EM GP |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm |
試料ステージ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER |
撮影 | フィルム・検出器のモデル: FEI FALCON III (4k x 4k) 検出モード: INTEGRATING / 平均電子線量: 43.0 e/Å2 |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |
-画像解析
粒子像選択 | 選択した数: 1961364 |
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初期 角度割当 | タイプ: MAXIMUM LIKELIHOOD / ソフトウェア - 名称: cryoSPARC |
最終 3次元分類 | クラス数: 3 / ソフトウェア - 名称: cryoSPARC |
最終 角度割当 | タイプ: MAXIMUM LIKELIHOOD / ソフトウェア - 名称: cryoSPARC |
最終 再構成 | 想定した対称性 - 点群: C1 (非対称) / 解像度のタイプ: BY AUTHOR / 解像度: 3.2 Å / 解像度の算出法: FSC 0.143 CUT-OFF / ソフトウェア - 名称: cryoSPARC (ver. 2.14) / 使用した粒子像数: 535633 |
-原子モデル構築 1
精密化 | プロトコル: OTHER |
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得られたモデル | PDB-7oya: |