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- PDB-7oyc: Cryo-EM structure of the Xenopus egg 80S ribosome -

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Basic information

Entry
Database: PDB / ID: 7oyc
TitleCryo-EM structure of the Xenopus egg 80S ribosome
Components
  • (40S ribosomal protein ...) x 26
  • (60S ribosomal protein ...) x 27
  • (Ribosomal protein ...) x 2
  • 18S rRNA18S ribosomal RNA
  • 28S rRNA28S ribosomal RNA
  • 5.8S rRNA5.8S ribosomal RNA
  • 5S rRNA5S ribosomal RNA
  • DNA-(apurinic or apyrimidinic site) lyaseDNA-(apurinic or apyrimidinic site) lyase
  • Death-associated protein-like 1-B
  • Eef2-prov protein
  • Eukaryotic translation initiation factor 5A
  • Gnb2l1-prov protein
  • HABP4_PAI-RBP1 domain-containing protein
  • KOW domain-containing protein
  • MGC116452 protein
  • MGC130910 protein
  • MGC85428 protein
  • Ribosomal_L16 domain-containing protein
  • Ribosomal_L18e/L15P domain-containing protein
  • Ribosomal_L23eN domain-containing protein
  • Ribosomal_S7 domain-containing protein
  • Rpl3-prov protein
  • Rpl32
  • Rpl37a
  • Rpl41
  • Rpl5-b protein
  • Rps1440S ribosomal protein S14
  • Rps16 protein40S ribosomal protein S16
  • TRASH domain-containing protein
KeywordsRIBOSOME / Eif5a / Eef2 / Habp4 / DapL1 / Dap1 / egg / Xenopus
Function / homology
Function and homology information


positive regulation of translational termination / positive regulation of translational elongation / protein-synthesizing GTPase / laminin receptor activity / mRNA transport / rough endoplasmic reticulum / laminin binding / translation elongation factor activity / nuclear pore / : ...positive regulation of translational termination / positive regulation of translational elongation / protein-synthesizing GTPase / laminin receptor activity / mRNA transport / rough endoplasmic reticulum / laminin binding / translation elongation factor activity / nuclear pore / : / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / DNA-(apurinic or apyrimidinic site) lyase / spindle / ribosomal small subunit assembly / cytosolic small ribosomal subunit / ribosome binding / protein transport / large ribosomal subunit / ribosome biogenesis / regulation of translation / cytoplasmic translation / small ribosomal subunit / 5S rRNA binding / cytosolic large ribosomal subunit / mitochondrial inner membrane / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / translation / cell division / DNA repair / GTPase activity / apoptotic process / GTP binding / endoplasmic reticulum membrane / RNA binding / zinc ion binding / metal ion binding / nucleus / plasma membrane / cytosol / cytoplasm
Similarity search - Function
DAP1/DAPL1 / Death-associated protein / Intracellular hyaluronan-binding protein 4, N-terminal domain / Intracellular hyaluronan-binding protein 4 N-terminal / Hyaluronan / mRNA binding family / RNA binding protein HABP4/SERBP1 / Ribosomal protein S14, type Z, archaeal / Translation elongation factor, IF5A, hypusine site / Eukaryotic initiation factor 5A hypusine signature. / Eukaryotic elongation factor 5A hypusine, DNA-binding OB fold ...DAP1/DAPL1 / Death-associated protein / Intracellular hyaluronan-binding protein 4, N-terminal domain / Intracellular hyaluronan-binding protein 4 N-terminal / Hyaluronan / mRNA binding family / RNA binding protein HABP4/SERBP1 / Ribosomal protein S14, type Z, archaeal / Translation elongation factor, IF5A, hypusine site / Eukaryotic initiation factor 5A hypusine signature. / Eukaryotic elongation factor 5A hypusine, DNA-binding OB fold / Translation elongation factor IF5A-like / Translation elongation factor, IF5A C-terminal / Eukaryotic elongation factor 5A hypusine, DNA-binding OB fold / Hyaluronan/mRNA-binding protein / Hyaluronan / mRNA binding family / Ubiquitin-like protein FUBI / 40S ribosomal protein SA / 40S ribosomal protein SA, C-terminal domain / 40S ribosomal protein SA C-terminus / Ribosomal protein L6, N-terminal / Ribosomal protein L6, N-terminal domain / Ribosomal protein L30e / Ribosomal protein L28e / Ribosomal protein L2, archaeal-type / Ribosomal L15/L27a, N-terminal / Elongation Factor G, domain II / Elongation Factor G, domain III / Ribosomal protein L23 / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / Translation elongation factor EFG/EF2, domain IV / Elongation factor G, domain IV / Elongation factor G, domain IV / Elongation factor G C-terminus / Elongation factor EFG, domain V-like / Elongation factor G C-terminus / EF-G domain III/V-like / Small (40S) ribosomal subunit Asc1/RACK1 / metallochaperone-like domain / TRASH domain / Ribosomal protein S21e, conserved site / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S19e, conserved site / Ribosomal protein S26e / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S25 / Ribosomal protein S26e signature. / Ribosomal protein S17e, conserved site / Ribosomal protein S2, eukaryotic / Tr-type G domain, conserved site / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Translational (tr)-type guanine nucleotide-binding (G) domain signature. / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein L29e / Ribosomal protein S3, eukaryotic/archaeal / S25 ribosomal protein / Ribosomal protein L10e, conserved site / Ribosomal protein L10e / Ribosomal protein L27e, conserved site / Ribosomal protein S19A/S15e / Ribosomal protein S21e signature. / Ribosomal L29e protein family / Ribosomal protein S30 / Ribosomal protein S3Ae, conserved site / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S19e / Ribosomal_S19e / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S8e, conserved site / 40S ribosomal protein S11, N-terminal / 40S ribosomal protein S1/3, eukaryotes / Ribosomal protein S6, eukaryotic / Ribosomal protein L24e, conserved site / Ribosomal protein S7e / 40S ribosomal protein S4, C-terminal domain / Ribosomal protein L34e, conserved site / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal S17 / Ribosomal protein S19e signature. / Ribosomal protein L44e / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal protein S19e / Ribosomal protein S27, zinc-binding domain superfamily / Ribosomal L38e protein family / Ribosomal L22e protein family / Ribosomal protein L23/L25, N-terminal / 40S Ribosomal protein S10 / 60S ribosomal protein L35
Similarity search - Domain/homology
: / : / : / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / 40S ribosomal protein S27 / 60S ribosomal protein L13a ...: / : / : / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / 40S ribosomal protein S27 / 60S ribosomal protein L13a / 40S ribosomal protein S29 / 60S ribosomal protein L10 / 40S ribosomal protein S5 / Ribosomal_L18e/L15P domain-containing protein / Ribosomal protein L15 / 60S ribosomal protein L14 / 60S ribosomal protein L32 / KOW domain-containing protein / 60S ribosomal protein L37a / 60S ribosomal protein L23a / 60S ribosomal protein L8 / ribosomal protein L24 L homeolog isoform X1 / HABP4_PAI-RBP1 domain-containing protein / 60S ribosomal protein L9 / 60S ribosomal protein L34 / Ribosomal protein L37 / 60S ribosomal protein L17 / 40S ribosomal protein S19 / Death-associated protein-like 1-B / 40S ribosomal protein S7 / 40S ribosomal protein S24 / 60S ribosomal protein L4-B / 60S ribosomal protein L35a / 40S ribosomal protein S15 / 40S ribosomal protein S20 / 40S ribosomal protein S13 / 40S ribosomal protein S4 / 60S ribosomal protein L22 / 40S ribosomal protein S10 / MGC130910 protein / Rps16 protein / MGC116452 protein / 60S ribosomal protein L35 / MGC116435 protein / 40S ribosomal protein S15a / 40S ribosomal protein S28 / 60S ribosomal protein L36 / 60S ribosomal protein L36a isoform X1 / 60S ribosomal protein L38 / 60S ribosomal protein L28 / 60S ribosomal protein L29 / 60S ribosomal protein L11 / 40S ribosomal protein S21 / 40S ribosomal protein S26 / 40S ribosomal protein S23 / 60S ribosomal protein L6 / 40S ribosomal protein S8 / 40S ribosomal protein S25 / 60S ribosomal protein L13 / 40S ribosomal protein S17 / 60S ribosomal protein L23 / 60S ribosomal protein L21 / 60S ribosomal protein L7a / Ubiquitin-60S ribosomal protein L40 / 40S ribosomal protein S18 / 60S ribosomal protein L31 / 60S ribosomal protein L27 / 40S ribosomal protein S11 / 60S ribosomal protein L27a / 60S ribosomal protein L18a / 60S ribosomal protein L30 / 40S ribosomal protein S2 / 40S ribosomal protein SA / Eukaryotic translation initiation factor 5A / Eef2-prov protein / Rpl3-prov protein / 60S ribosomal protein L19 / DNA-(apurinic or apyrimidinic site) lyase / 40S ribosomal protein S6 / 40S ribosomal protein S9 / 40S ribosomal protein S3a-A / 60S ribosomal protein L5-B / Gnb2l1-prov protein
Similarity search - Component
Biological speciesXenopus laevis (African clawed frog)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.4 Å
AuthorsLeesch, F. / Lorenzo-Orts, L. / Grishkovskaya, I. / Kandolf, S. / Belacic, K. / Meinhart, A. / Haselbach, D. / Pauli, A.
Funding support Austria, Switzerland, 5items
OrganizationGrant numberCountry
Austrian Research Promotion AgencyFFG-852936 Austria
Austrian Science FundSTART Projekt Y 1031-B28 Austria
Austrian Science FundSFB RNA-Deco F 80 Austria
Swiss National Science FoundationP2GEP3_191204 Switzerland
European Molecular Biology Organization (EMBO)ALTF 1165-2019
CitationJournal: Nature / Year: 2023
Title: A molecular network of conserved factors keeps ribosomes dormant in the egg.
Authors: Friederike Leesch / Laura Lorenzo-Orts / Carina Pribitzer / Irina Grishkovskaya / Josef Roehsner / Anastasia Chugunova / Manuel Matzinger / Elisabeth Roitinger / Katarina Belačić / Susanne ...Authors: Friederike Leesch / Laura Lorenzo-Orts / Carina Pribitzer / Irina Grishkovskaya / Josef Roehsner / Anastasia Chugunova / Manuel Matzinger / Elisabeth Roitinger / Katarina Belačić / Susanne Kandolf / Tzi-Yang Lin / Karl Mechtler / Anton Meinhart / David Haselbach / Andrea Pauli /
Abstract: Ribosomes are produced in large quantities during oogenesis and are stored in the egg. However, the egg and early embryo are translationally repressed. Here, using mass spectrometry and cryo-electron ...Ribosomes are produced in large quantities during oogenesis and are stored in the egg. However, the egg and early embryo are translationally repressed. Here, using mass spectrometry and cryo-electron microscopy analyses of ribosomes isolated from zebrafish (Danio rerio) and Xenopus laevis eggs and embryos, we provide molecular evidence that ribosomes transition from a dormant state to an active state during the first hours of embryogenesis. Dormant ribosomes are associated with four conserved factors that form two modules, consisting of Habp4-eEF2 and death associated protein 1b (Dap1b) or Dap in complex with eIF5a. Both modules occupy functionally important sites and act together to stabilize ribosomes and repress translation. Dap1b (also known as Dapl1 in mammals) is a newly discovered translational inhibitor that stably inserts into the polypeptide exit tunnel. Addition of recombinant zebrafish Dap1b protein is sufficient to block translation and reconstitute the dormant egg ribosome state in a mammalian translation extract in vitro. Thus, a developmentally programmed, conserved ribosome state has a key role in ribosome storage and translational repression in the egg.
History
DepositionJun 24, 2021Deposition site: PDBE / Processing site: PDBE
Revision 1.0Jul 20, 2022Provider: repository / Type: Initial release
Revision 1.1Feb 1, 2023Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2Feb 8, 2023Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
11: Eukaryotic translation initiation factor 5A
22: 18S rRNA
51: 28S rRNA
71: 5S rRNA
81: 5.8S rRNA
A1: 60S ribosomal protein L8
A2: 40S ribosomal protein SA
B1: Rpl3-prov protein
B2: 40S ribosomal protein S3a-A
C1: 60S ribosomal protein L4-B
C2: 40S ribosomal protein S2
D1: Rpl5-b protein
D2: DNA-(apurinic or apyrimidinic site) lyase
E1: 60S ribosomal protein L6
E2: 40S ribosomal protein S4
F1: MGC130910 protein
F2: Ribosomal_S7 domain-containing protein
G1: 60S ribosomal protein L7a
G2: 40S ribosomal protein S6
H1: 60S ribosomal protein L9
H2: 40S ribosomal protein S7
I1: Ribosomal_L16 domain-containing protein
I2: 40S ribosomal protein S8
J1: 60S ribosomal protein L11
J2: 40S ribosomal protein S9
K2: 40S ribosomal protein S10
L1: 60S ribosomal protein L13
L2: 40S ribosomal protein S11
M1: 60S ribosomal protein L14
N1: Ribosomal protein L15
N2: 40S ribosomal protein S13
O1: 60S ribosomal protein L13a
O2: Rps14
P1: 60S ribosomal protein L17
P2: 40S ribosomal protein S15
Q1: Ribosomal_L18e/L15P domain-containing protein
Q2: Rps16 protein
R1: 60S ribosomal protein L19
R2: 40S ribosomal protein S17
S1: 60S ribosomal protein L18a
S2: 40S ribosomal protein S18
T1: 60S ribosomal protein L21
T2: 40S ribosomal protein S19
U1: 60S ribosomal protein L22
U2: 40S ribosomal protein S20
V1: 60S ribosomal protein L23
V2: 40S ribosomal protein S21
W1: TRASH domain-containing protein
W2: 40S ribosomal protein S15a
X1: Ribosomal_L23eN domain-containing protein
X2: 40S ribosomal protein S23
Y1: KOW domain-containing protein
Y2: 40S ribosomal protein S24
Z1: 60S ribosomal protein L27
Z2: 40S ribosomal protein S25
a1: 60S ribosomal protein L27a
a2: 40S ribosomal protein S26
b1: 60S ribosomal protein L29
b2: 40S ribosomal protein S27
c1: 60S ribosomal protein L30
c2: 40S ribosomal protein S28
d1: 60S ribosomal protein L31
d2: 40S ribosomal protein S29
e1: Rpl32
e2: 40S ribosomal protein S30
f1: 60S ribosomal protein L35a
g1: 60S ribosomal protein L34
g2: Gnb2l1-prov protein
h1: 60S ribosomal protein L35
i1: 60S ribosomal protein L36
i2: HABP4_PAI-RBP1 domain-containing protein
j1: Ribosomal protein L37
k1: 60S ribosomal protein L38
l1: MGC116452 protein
m1: 60S ribosomal protein L40
n1: Rpl41
o1: MGC85428 protein
p1: Rpl37a
r1: 60S ribosomal protein L28
s1: Death-associated protein-like 1-B
v2: Eef2-prov protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)3,587,981222
Polymers3,584,26681
Non-polymers3,715141
Water0
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: mass spectrometry
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

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Protein , 21 types, 21 molecules 11B1D1D2F1F2I1O2Q1Q2W1X1Y1e1g2i2l1o1p1s1v2

#1: Protein Eukaryotic translation initiation factor 5A / eIF-5A


Mass: 16813.344 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q7ZXG3
#8: Protein Rpl3-prov protein


Mass: 46144.133 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q7ZYR1
#12: Protein Rpl5-b protein


Mass: 34066.246 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q8AVI7
#13: Protein DNA-(apurinic or apyrimidinic site) lyase / DNA-(apurinic or apyrimidinic site) lyase


Mass: 27026.682 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg
References: UniProt: Q7ZYT3, DNA-(apurinic or apyrimidinic site) lyase
#16: Protein MGC130910 protein


Mass: 28762.365 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q3B8I3
#17: Protein Ribosomal_S7 domain-containing protein


Mass: 22780.285 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: A0A1L8F8E2
#22: Protein Ribosomal_L16 domain-containing protein


Mass: 24634.074 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: A0A1L8F5F1
#33: Protein Rps14 / 40S ribosomal protein S14


Mass: 16302.772 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg
#36: Protein Ribosomal_L18e/L15P domain-containing protein


Mass: 21659.750 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: A0A1L8FN04
#37: Protein Rps16 protein / 40S ribosomal protein S16


Mass: 16361.231 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q3KQC9
#48: Protein TRASH domain-containing protein


Mass: 17721.992 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: A0A1L8HGE0
#50: Protein Ribosomal_L23eN domain-containing protein


Mass: 17718.117 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: A0A1L8H4S4
#52: Protein KOW domain-containing protein


Mass: 17235.285 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: A0A1L8GXU8
#64: Protein Rpl32


Mass: 15818.773 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: A0A1L8GHL2
#68: Protein Gnb2l1-prov protein / LOC446289 protein


Mass: 35107.613 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q8AVP6
#71: Protein HABP4_PAI-RBP1 domain-containing protein


Mass: 42746.195 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: A0A1L8HPG3
#74: Protein MGC116452 protein / MGC116477 protein


Mass: 6398.745 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q4FZP7
#77: Protein MGC85428 protein


Mass: 12496.923 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / References: UniProt: Q66KU5
#78: Protein Rpl37a


Mass: 10283.390 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: A0A1L8H102
#80: Protein Death-associated protein-like 1-B


Mass: 12548.723 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: A3KMU5
#81: Protein Eef2-prov protein


Mass: 95552.305 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Eggs / References: UniProt: Q7ZXP8

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RNA chain , 4 types, 4 molecules 22517181

#2: RNA chain 18S rRNA / 18S ribosomal RNA


Mass: 588678.000 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: GenBank: X04025.1
#3: RNA chain 28S rRNA / 28S ribosomal RNA


Mass: 1333067.250 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: GenBank: X59734.1
#4: RNA chain 5S rRNA / 5S ribosomal RNA


Mass: 38682.953 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: GenBank: M10635.1
#5: RNA chain 5.8S rRNA / 5.8S ribosomal RNA


Mass: 50198.691 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: GenBank: X02995.1

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60S ribosomal protein ... , 27 types, 27 molecules A1C1E1G1H1J1L1M1O1P1R1S1T1U1V1Z1a1b1c1d1f1g1h1i1k1m1r1

#6: Protein 60S ribosomal protein L8 /


Mass: 27980.740 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: A0A1L8H6H2
#10: Protein 60S ribosomal protein L4-B / Ribosome / L1B


Mass: 45525.805 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: P02385
#14: Protein 60S ribosomal protein L6 /


Mass: 29743.496 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q6DJF7
#18: Protein 60S ribosomal protein L7a /


Mass: 29939.656 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q6GQB2
#20: Protein 60S ribosomal protein L9 /


Mass: 21934.648 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: A0A1L8HTS7
#24: Protein 60S ribosomal protein L11 /


Mass: 20215.475 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q66KY6
#27: Protein 60S ribosomal protein L13 /


Mass: 24257.652 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q6DJN5
#29: Protein 60S ribosomal protein L14 /


Mass: 16174.283 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: A0A1L8FWH1
#32: Protein 60S ribosomal protein L13a /


Mass: 26450.830 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / References: UniProt: A0A1L8ENM5
#34: Protein 60S ribosomal protein L17 /


Mass: 21457.219 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: A0A1L8I3J8
#38: Protein 60S ribosomal protein L19 /


Mass: 23550.256 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q7ZYS1
#40: Protein 60S ribosomal protein L18a /


Mass: 20697.473 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q7SZB7
#42: Protein 60S ribosomal protein L21 /


Mass: 18706.986 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q6GP41
#44: Protein 60S ribosomal protein L22 /


Mass: 14842.979 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: P50886
#46: Protein 60S ribosomal protein L23 /


Mass: 14878.478 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q6GNH1
#54: Protein 60S ribosomal protein L27 /


Mass: 15864.831 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q6PHL5
#56: Protein 60S ribosomal protein L27a /


Mass: 16636.592 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q7SZB2
#58: Protein 60S ribosomal protein L29 /


Mass: 8596.122 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q66KW1
#60: Protein 60S ribosomal protein L30 /


Mass: 12959.299 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q7SZC0
#62: Protein 60S ribosomal protein L31 /


Mass: 14548.990 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q6NUH0
#66: Protein 60S ribosomal protein L35a / / L32


Mass: 12630.784 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: P02434
#67: Protein 60S ribosomal protein L34 /


Mass: 13325.087 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: A0A1L8HUR4
#69: Protein 60S ribosomal protein L35 /


Mass: 14492.458 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q4FZQ7
#70: Protein 60S ribosomal protein L36 /


Mass: 12362.991 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q66KU4
#73: Protein 60S ribosomal protein L38 /


Mass: 8226.934 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q66KV2
#75: Protein 60S ribosomal protein L40 / / CEP52 / Ubiquitin / Ubiquitin-60S ribosomal protein L40


Mass: 14758.394 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q6GQF3
#79: Protein 60S ribosomal protein L28 /


Mass: 15587.421 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q66KV6

+
40S ribosomal protein ... , 26 types, 26 molecules A2B2C2E2G2H2I2J2K2L2N2P2R2S2T2U2V2W2X2Y2Z2a2b2c2d2e2

#7: Protein 40S ribosomal protein SA / / 37 kDa laminin receptor precursor / 37LRP / 37/67 kDa laminin receptor / LRP/LR / 67 kDa laminin ...37 kDa laminin receptor precursor / 37LRP / 37/67 kDa laminin receptor / LRP/LR / 67 kDa laminin receptor / 67LR / Laminin receptor 1 / LamR / Laminin-binding protein precursor p40 / LBP/p40


Mass: 34027.176 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q7ZX07
#9: Protein 40S ribosomal protein S3a-A / Ribosome


Mass: 29998.049 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q801S3
#11: Protein 40S ribosomal protein S2 /


Mass: 30326.236 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q7T0R9
#15: Protein 40S ribosomal protein S4 /


Mass: 29722.881 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: P49401
#19: Protein 40S ribosomal protein S6 /


Mass: 28778.766 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q7ZYU0
#21: Protein 40S ribosomal protein S7 / / 40S ribosomal protein S8


Mass: 22226.008 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: P02362
#23: Protein 40S ribosomal protein S8 /


Mass: 24109.258 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q6DJI0
#25: Protein 40S ribosomal protein S9 /


Mass: 22627.576 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q7ZYU4
#26: Protein 40S ribosomal protein S10 /


Mass: 18918.830 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q07254
#28: Protein 40S ribosomal protein S11 /


Mass: 18428.641 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q7SZ77
#31: Protein 40S ribosomal protein S13 /


Mass: 17287.402 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: P49393
#35: Protein 40S ribosomal protein S15 / / RIG protein


Mass: 17010.188 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: P20342
#39: Protein 40S ribosomal protein S17 /


Mass: 15552.119 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q6GNF4
#41: Protein 40S ribosomal protein S18 /


Mass: 17717.738 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q6NTT2
#43: Protein 40S ribosomal protein S19 /


Mass: 16079.565 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: A0A310TRG4
#45: Protein 40S ribosomal protein S20 / / S22


Mass: 13381.735 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: P23403
#47: Protein 40S ribosomal protein S21 /


Mass: 9227.467 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q6AZJ9
#49: Protein 40S ribosomal protein S15a /


Mass: 14881.555 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q4QR44
#51: Protein 40S ribosomal protein S23 /


Mass: 15803.570 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q6AZL9
#53: Protein 40S ribosomal protein S24 / / S19


Mass: 15324.179 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: P02377
#55: Protein 40S ribosomal protein S25 /


Mass: 13863.302 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q6DJJ1
#57: Protein 40S ribosomal protein S26 /


Mass: 13125.560 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q6AZL7
#59: Protein 40S ribosomal protein S27 /


Mass: 9480.186 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: A0A1L8EJM5
#61: Protein 40S ribosomal protein S28 /


Mass: 7852.051 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q66KP7
#63: Protein 40S ribosomal protein S29 /


Mass: 10202.862 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: A0A1L8F058
#65: Protein 40S ribosomal protein S30 /


Mass: 14537.767 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: Q4KLF0

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Ribosomal protein ... , 2 types, 2 molecules N1j1

#30: Protein Ribosomal protein L15 /


Mass: 24119.254 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: A0A1L8FRG2
#72: Protein Ribosomal protein L37 /


Mass: 11030.922 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg / References: UniProt: A0A1L8I2F1

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Protein/peptide , 1 types, 1 molecules n1

#76: Protein/peptide Rpl41


Mass: 3473.451 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Xenopus laevis (African clawed frog) / Tissue: Egg

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Non-polymers , 2 types, 141 molecules

#82: Chemical...
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 134 / Source method: obtained synthetically / Formula: Mg
#83: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 7 / Source method: obtained synthetically / Formula: Zn

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Xenopus egg 80S ribosome / Type: RIBOSOME / Entity ID: #1-#81 / Source: NATURAL
Molecular weightExperimental value: NO
Source (natural)Organism: Xenopus laevis (African clawed frog) / Tissue: Egg
Buffer solutionpH: 7.6
Buffer component
IDConc.NameFormulaBuffer-ID
15 mMMagnesium acetateMg(OAc)21
220 mMHEPES1
3100 mMpotassium cholrideKCl1
41 mMDithiotreitolDTT1
510 mMammonium cholrideNH4Cl1
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportGrid material: COPPER / Grid mesh size: 200 divisions/in. / Grid type: Quantifoil R3.5/1
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / C2 aperture diameter: 70 µm / Alignment procedure: ZEMLIN TABLEAU
Specimen holderCryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image recordingAverage exposure time: 1 sec. / Electron dose: 40 e/Å2 / Detector mode: INTEGRATING / Film or detector model: FEI FALCON III (4k x 4k) / Num. of grids imaged: 1

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Processing

SoftwareName: UCSF ChimeraX / Version: 0.91/v8 / Classification: model building / URL: https://www.rbvi.ucsf.edu/chimerax/ / Os: macOS / Type: package
EM software
IDNameVersionCategory
2EPUimage acquisition
4CTFFIND4CTF correction
10cryoSPARC2.14initial Euler assignment
11cryoSPARC2.14final Euler assignment
12cryoSPARC2.14classification
13cryoSPARC3D reconstruction
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
SymmetryPoint symmetry: C1 (asymmetric)
3D reconstructionResolution: 2.4 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 412340 / Symmetry type: POINT

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